LOCUS       sequence-314742-       24111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recipient plant transformation vector containing selection markers 
            and a Cas9 expression cassette, but lacking guide RNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 24111)
  AUTHORS   Stuttmann J, Barthel K, Martin P, Ordon J, Erickson JL, Herr R, 
            Ferik F, Kretschmer C, Berner T, Keilwagen J, Marillonnet S, Bonas U
  TITLE     Highly efficient multiplex editing: One-shot generation of 8x 
            Nicotiana benthamiana and 12x Arabidopsis mutants.
  JOURNAL   Plant J. 2021 Feb 12. doi: 10.1111/tpj.15197.
  PUBMED    33577114
REFERENCE   2  (bases 1 to 24111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2021 Feb 12. doi: 10.1111/tpj.15197."
COMMENT     Sequence Label: pDGE669
FEATURES             Location/Qualifiers
     source          1..24111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1062..1691
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2120..3193
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3259..3453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3689..3711)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3797..3937
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3852..3871
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3952..3969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4123..4711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4203..4222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4957..5748)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASCADQLEEFVHYVKGEITKVVGK"
     misc_feature    6269..6293
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(6365..6617)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6630..7655)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6630..7655)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    7657..7712
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(7748..7927)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             complement(8566..9294)
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLNGMDELYK"
     CDS             complement(9307..10038)
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     terminator      complement(11667..12374)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(15623..15643)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(21376..21396)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(21502..21847)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(21539..21559)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        22286..22388
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             22389..23072
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(22455..22474)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             23392..23697
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     23576..23595
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    23818..23842
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
       61 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
      121 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
      181 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
      241 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
      301 cagcacccgc gacctactgg acattgccga gagcatccag gaggccggcg cgggcctgcg
      361 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
      421 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
      481 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
      541 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
      601 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
      661 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
      721 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
      781 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
      841 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
      901 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
      961 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     1021 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     1081 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     1141 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     1201 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     1261 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     1321 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     1381 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     1441 aggtcacgga tggaaggcta caagcggcct ttgtagtgtc gcgggcgatc aaaggcacgc
     1501 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     1561 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     1621 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     1681 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     1741 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     1801 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     1861 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     1921 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     1981 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     2041 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
     2101 ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc
     2161 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
     2221 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
     2281 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
     2341 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
     2401 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
     2461 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
     2521 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
     2581 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
     2641 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
     2701 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
     2761 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
     2821 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
     2881 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
     2941 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
     3001 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
     3061 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
     3121 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
     3181 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
     3241 tcgaaaaggt gtctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
     3301 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
     3361 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
     3421 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
     3481 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
     3541 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
     3601 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
     3661 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     3721 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     3781 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     3841 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     3901 accgcacaga tgcgtaagga gaaaataccg cataaggcgc tcttccgctt cctcgctcac
     3961 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4561 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatg atatatctcc
     4801 caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact tgacctgata
     4861 gtttggctgt gagcaattat gtgcttagtg catctaacgc ttgagttaag ccgcgccgcg
     4921 aagcggcgtc ggcttgaacg aatttctagc tagacattat ttgccgacta ccttggtgat
     4981 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcacgagg ccaagcgatc
     5041 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     5101 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     5161 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     5221 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     5281 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     5341 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     5401 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     5461 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     5521 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     5581 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     5641 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     5701 tacctctgat agttgagtcg atacttcggc gatcaccgct tcccccatga tgtttaactt
     5761 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     5821 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     5881 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     5941 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     6001 ggcttatgtc cactgggttc gtgcccgaat tgatcacagg cagcaacgct ctgtcatcgt
     6061 tacaatcagc atgctaccct ccgcgagatc atccgtgttt aaaacccggc agcttagttg
     6121 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
     6181 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
     6241 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga
     6301 cgcttagaca acttaataac acattgcgga tccctcggtc acatgtgcat cctccacaag
     6361 cgtcgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
     6421 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
     6481 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
     6541 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
     6601 caaatgtttg aacgatctgc ttgacaagcc tattcctttg ccctcggacg agtgctgggg
     6661 cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt
     6721 ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat
     6781 cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg
     6841 tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc
     6901 ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag
     6961 atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct
     7021 gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc
     7081 cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac
     7141 gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg
     7201 catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac
     7261 ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc
     7321 agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg
     7381 gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg
     7441 agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag
     7501 ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct
     7561 tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc
     7621 tcgacagacg tcgcggtgag ttcaggcttt ttcattgtaa ttgtaaatag taattgtaat
     7681 gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacagtatga gagtgaatat
     7741 gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag
     7801 aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg
     7861 acgtatgtgc ttagctcatt aaactccaga aacccgcggc tgagtggctc cttcaacgtt
     7921 gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa
     7981 cgtgactccc ttaattctcc gctctccgca tgcgaattct ctgagcggta cactgccaga
     8041 tgaagccgaa ggagtacttt aatatactag gtctggcgat atgatcaatt tgaaaatata
     8101 gagtcataac tgtagaaatg gaatcagatt ccattacaaa aagagataaa tctcaacttt
     8161 aaattttttc aggaagaaaa aaatgtttct tctaatcttc gtgttttact ggccttctca
     8221 taaaatggtt tgatataaaa gaaaattaaa taagaaaaag agaaaacaaa aggctagcca
     8281 gagagaggag aaaatatata ataatataac ttagtttatt catctcataa tctctgcatt
     8341 ttgcaaggag atcgaaactg agcaaaacat acacaaacag agaaataaaa tatagggtac
     8401 aaacaatatc aatagcttag acaacatgaa gctacaaaag aacttcgcct cctgtggggc
     8461 ttattattta ctcttttttt tttcttattc ttcgtctttt ctcattgcct ctcgaaattg
     8521 gaaaaagcct ttcttttagc tcaacattct gcttttccaa gctcatttgt acagctcgtc
     8581 catgccatta agtttgtgcc ccagtttgct agggaggtcg cagtatctgg ccacagccac
     8641 ctcgtgctgc tcgacgtagg tttctttgtc ggcctccttg attctttcca gtctgtggtc
     8701 cacatagtag acgccgggca tcttgaggtt cttagcgggt ttcttggatc tgtatgtggt
     8761 cttgaagttg cagatcaggt ggcccccgcc cacgagcttc agggccatgt cggttctgcc
     8821 ttccaggccg ccgtcagcgg ggtacagcat ctcggtgttg gcctcccagc cgagtgtttt
     8881 cttctgcatc acagggccgt tggatgggaa gttcacccct ctgatcttga cgttgtagat
     8941 gaggcagccg tcctggaggc tggtgtcctg ggtagcggtc agcacgcccc cgtcctcgta
     9001 tgtggtgact ctctcccatg tgaagccctc agggaaggac tgcttaaaga agtcggggat
     9061 gccctgggtg tggttgatga aggttctgct gccgtacatg aagctggtag ccaggatgtc
     9121 gaaggcgaag gggagagggc cgccctcgac caccttgatt ctcatggtct gggtgccctc
     9181 gtagggcttg ccttcgccct cggatgtgca cttgaagtgg tggttgttca cggtgccctc
     9241 catgtacagc ttcatgtgca tgttctcctt aatcagctct tcgcccttag acacggcagc
     9301 cgaagctttg tacagctcgt ccatgccatt aagtttgtgc cccagtttgc tagggaggtc
     9361 gcagtatctg gccacagcca cctcgtgctg ctcgacgtag gtttctttgt cggcctcctt
     9421 gattctttcc agtctgtggt ccacatagta gacgccgggc atcttgaggt tcttagcggg
     9481 tttcttggat ctgtatgtgg tcttgaagtt gcagatcagg tggcccccgc ccacgagctt
     9541 cagggccatg tcggttctgc cttccaggcc gccgtcagcg gggtacagca tctcggtgtt
     9601 ggcctcccag ccgagtgttt tcttctgcat cacagggccg ttggatggga agttcacccc
     9661 tctgatcttg acgttgtaga tgaggcagcc gtcctggagg ctggtgtcct gggtagcggt
     9721 cagcacgccc ccgtcctcgt atgtggtgac tctctcccat gtgaagccct cagggaagga
     9781 ctgcttaaag aagtcgggga tgccctgggt gtggttgatg aaggttctgc tgccgtacat
     9841 gaagctggta gccaggatgt cgaaggcgaa ggggagaggg ccgccctcga ccaccttgat
     9901 tctcatggtc tgggtgccct cgtagggctt gccttcgccc tcggatgtgc acttgaagtg
     9961 gtggttgttc acggtgccct ccatgtacag cttcatgtgc atgttctcct taatcagctc
    10021 ttcgccctta gacaccattt ttgctatttg tgtatgtttt cttgttttgg tttgagtgaa
    10081 gagtgtagtg aagggttggt ttgtatttat aggtgagaaa ggagatttgc atggcaatca
    10141 cgtgtaagaa tgcatgcatg catgagtgtg tgtggagaga gagagatgct cttacgttgt
    10201 tgtcgggatt atgtgttgga aaagagtgac aagtgacgaa caaaacaata ccactcatca
    10261 tagctccgct tatgcatgct atgtgtgatt tgtagctctt gttctgacat gacactatga
    10321 acaatttttt aaaaagaagg ttaaactttt atttaaataa aaaccctatg ttaatttggt
    10381 ttcaatcgag taagatgaga agtcaatctt ttgatggtag ctagtgatgt tggaaatata
    10441 gttgtgccag cttaatctga aatttagaat ataattttat aagagtatct atcaatatat
    10501 atattaacgg gtcactcacg aacaatgcat gtaagaaatt tttatgaggg atatatatgt
    10561 taagaaaata ccgaattata tgtgtacgta tacacacata attagaggtg tcaaaataca
    10621 tcaaaactcg tggttaaggg tttaccaaaa aaattataac tcaaaccgtg aagtaggagt
    10681 aaaagaaatt accaaaacca tgttttgaca taaaattgaa aacagagcga tgaagagaat
    10741 aagaagatga tacgtacaaa atcttacgag ttatgaatat cgagagtttt acaccaacaa
    10801 atcaaaaacc aaaatcgaga gttcgtacgt ctcgactaaa tcaaagctag caattcttag
    10861 ctatgttttt catttcaaag agttaccaaw awraaaaaaa aaaaaaaaaa aaaagagtta
    10921 ccactatgaa ttactcaaag agtaaacaat atgaataata caaatccgat cgatatcact
    10981 gatcaagaaa cacattatat tctaactttc taaccacggt tagcttgaac caaactaaac
    11041 cttctcaaca ccagagcaat ggccttacat aaattctcat cagaaaatgg tcgaccagct
    11101 gttaaccaga actaattgta ttcacagaac aacatcaaca cgtttttttt ataaagacat
    11161 ttgatcggta cgttttacac tcttggccaa aatggctagc acttttttac cacaattgta
    11221 agcgactatc gatcaaaccg atcatgtaat gtaaaatttc gtttttgaac ttcgcttcca
    11281 atggatcatc taatttaaaa ttaaaatttg gcatgtctct tccacttgat gccgtgcaat
    11341 ttcttgagcc caaaaaatag atattaacac gcatgcggtg gggcggtggt aactagacta
    11401 tggcgtaaat tcacacatgc ataatgcttc attctcatag cgaataaatt ttcttcgtgt
    11461 tgggtcacta attggatctg tgctttggtt ttgtgtttta tccatggtgt tgtgttcctt
    11521 gattatatca tatggataaa tgtttaatac ttgatagttg atcagatgat atagaactag
    11581 ttgcaaatct atcatcttga atgaaaacac accgtctgtt agactcgcaa atttatcttc
    11641 tccccatggg aattcgtaaa gcggtcctgc tgagcctcga catgttgtcg caaaattcgc
    11701 cctggacccg cccaacgatt tgtcgtcact gtcaaggttt gacctgcact tcatttgggg
    11761 cccacataca ccaaaaaaat gctgcataat tctcggggca gcaagtcggt tacccggccg
    11821 ccgtgctgga ccgggttgaa tggtgcccgt aactttcggt agagcggacg gccaatactc
    11881 aacttcaagg aatctcaccc atgcgcgccg gcggggaacc ggagttccct tcagtgagcg
    11941 ttattagttc gccgctcggt gtgtcgtaga tactagcccc tggggcactt ttgaaatttg
    12001 aataagattt atgtaatcag tcttttaggt ttgaccggtt ctgccgcttt ttttaaaatt
    12061 ggatttgtaa taataaaacg caattgtttg ttattgtggc gctctatcat agatgtcgct
    12121 ataaacctat tcagcacaat atattgtttt cattttaata ttgtacatat aagtagtagg
    12181 gtacaatcag taaattgaac ggagaatatt attcataaaa atacgatagt aacgggtgat
    12241 atattcatta gaatgaaccg aaaccggcgg taaggatctg agctacacat gctcaggttt
    12301 tttacaacgt gcacaacaga attgaaagca aatatcatgc gatcataggc ttctcgcata
    12361 tctcattaaa gcaggacaag cctacatttg ttctttccaa attttggcaa tatcttgtgc
    12421 aacattagtg gcatctatag aagcaccaaa cagtcctatt cctgtaaatc caactgcata
    12481 cagaccatcc tcccccttcc aaccatttgg gaatgggctt tttggacatc cctcccttga
    12541 aaacaattca ctctcctgta tcagtaaaat tctcattaga ccaaatttcg aagggggcta
    12601 ttatagaacg agagaaagtt gctcagatgg taaagagccc cttattccca atcttaagat
    12661 tgtgagttcg agttatcaaa agaacaaaaa ggtatgagga aagggtaaaa gaaaaaagac
    12721 ggttatagtg aattctaact aattaataac tcaaaggaaa gaatccacag gataatgaga
    12781 taccaactta catgaatgta acaagaatcc ataaataatt actacattca ttaagagttc
    12841 atttgactaa atcatccttt atagttgaag gagcttttat catacttagt actatatata
    12901 atttatgatt aaacaccaat ttaattaggc atagaaataa aacttgtgta tttccttacc
    12961 attaaccaag aagttacatt gctggtataa ccagttgcca agataacaga gtcgatctct
    13021 agaatttgtc cattaacaaa ttctactttt ccttgagtaa atttcttgat tgctggaact
    13081 atgtttattt cctcagggaa attacgaccc tgtacctgaa agattaacaa aactagatga
    13141 tatcattcat gtgtgaatat aatatattca tgcgagcaaa tgaaagatac aagatcactt
    13201 tctagtgtta cttgaaaaat aatacttatt gaatataaaa cttaccgagc ttcgaactac
    13261 catgaatgga tttgcattat gttgggaaag atcaagtgag atatcgatcc cggaattgcc
    13321 acagccaacc gccagcacat tttctccagt atagtattcc ccagtcttgt actcacaagc
    13381 atgaataacc tggccaccaa aatcttgcaa tccttcgaat tcgggcacta tcatctcagc
    13441 attctctcct gtggccacaa taagccactt acacatgtat tcagaggttg aaccattgat
    13501 ttcagaaact gttttcacct tccataaacc acatgtctca tcatatccag ctaagtttac
    13561 tgactcgttg agttgtggtt tgatctcgaa atgctttgca taagatacga ggtagctgat
    13621 gaattggttt ttggttggat actctggaaa gtctggtgga aatggcaagc caggcaattc
    13681 gcagtattgt cgtggcacgt taagcctaag ccgatcgtag gtcttgtgtt gccacagaga
    13741 agcaatgcag tccgcgcgtt caatgattac atacggaaca ctgtattgct taaggacagc
    13801 agcagtagcc aggcctgaag ggccagctcc aacaataaga ggaccattta cttgtatgca
    13861 tttagcagca caagaggatt tttttggttc aagtgcatca acagttagag gtgaacaaga
    13921 attaaagcaa ttctgattca tcattaaata tatgtgcaac taggactact tgtgttttga
    13981 tagatttagc gggtgacaag aacaagagga gaaaagagaa aggggatgat aacttgaagt
    14041 tgtgaggatg gttaggttta tataataaaa ttggtcaggc aaacgtgttc attgtttaac
    14101 cagggtgtgc aaattgtggt ttaacccata aactgaacca gaaatggtta atctatcggt
    14161 taaacggttt tattgttttt tttttttttt ngatatattg atctttcagt tcaatgatta
    14221 gttctttgag tttacttgca tactaatttc atatttccct tgcataagtt tcgttagctt
    14281 gactatgata tattagagtt tgctataagg gaaaagatat acaaatgtat cgttgtatct
    14341 gtggtaaaaa gcaagtacaa tctaatgtgt catgtatcat tcgcatttca aagtaaaact
    14401 aaggagttta gtctaggttg atattaatag actgttgttt tgcattaatt ctttgttgcg
    14461 gttggcagta ccaagcgttg gtaagggaaa tgattgaatt ctattttact tgatattttt
    14521 ttctagttgg aaactcagga acaaatgttt tcttactaga aaaaataatt agaaacaaaa
    14581 gcagcatatg aggaatcttc aaatacatgt gaattagaac tcttgacacc aaaagtttca
    14641 acgcaacaag gagatgagca aatgtgatct ccacatttgc ttcctatatc actctctcat
    14701 tacaagcagc aaaccacaac aaaaaatttc gcctatgata aagtatgctt gatttcactc
    14761 cacaaggcat cccaaagatg ttgatctagc cgtcactcta agccaccaaa cacagacacc
    14821 attaatttat catgatactt ttctggataa attctgaaaa ccagagccaa gacacatgag
    14881 attgacagtt cctgtggagg catcaggttt catgacaaac ttaacaccca aaaaatctct
    14941 gatgtgcctt aatgctgctc cgagctcgaa ttctagtagc gttgtcaatc aattggcaag
    15001 tcataaaatg cattaaaaaa tattttcata ctcaactaca aatccatgag tataactata
    15061 attataaagc aatgattaga atctgacaag gattctggaa aattacataa aggaaagttc
    15121 ataaatgtct aaaacacaag aggacatact tgtattcagt aacatttgca gcttttctag
    15181 gtctgaaaat atatttgttg cctagtgaat aagcataatg gtacaactac aagtgtttta
    15241 ctcctcatat taacttcggt cattagaggc cacgatttga cacattttta ctcaaaacaa
    15301 aatgtttgca tatctcttat aatttcaaat tcaacacaca acaaataaga gaaaaaacaa
    15361 ataatattaa tttgagaatg aacaaaagga ccatatcatt cattaactct tctccatcca
    15421 tttccatttc acagttcgat agcgaaaacc gaataaaaaa cacagtaaat tacaagcaca
    15481 acaaatggta caagaaaaac agttttccca atgccataat actcaaactc agtaggattc
    15541 tggtgtgtgc gcaatgaaac tgatgcattg aacttgacga acgttgtcga aaccgatgat
    15601 acgaacgaaa gctctaagct cagactttac gcttcttctt tgggtcagcc ctagagtcac
    15661 cacccaactg cgacagatct atccttgtct catagagccc tgtaatagat tggtgaataa
    15721 gggtagcgtc caacacttcc ttggttgatg tgtagcgctt tctgtcgata gtggtatcga
    15781 agtacttaaa ggctgctggg gctcccagat tggtcagtgt gaacaggtgg atgatgtttt
    15841 ccgcttgttc ccttatcggc ttatctctgt gcttgttgta cgcggaaagc acctttaatt
    15901 gtagaaaacc agtaaaacat ggacaattag gaggcatcga ttatctaacc aaagacttta
    15961 acaacactaa tctgccacta atattcatga gaagtcctta ccttatcgag gttagcgtct
    16021 gcaagaatga cccgcttgga aaattcgctg atctgctcta tgatctcatc aagatagtgc
    16081 ttgtgctgct cgacaaacaa ctgcttctgc tcgttgtcct ctggtgaccc cttcagcttc
    16141 tcatagtgtg aggccaagta caggaagttg acgtacttac tgggcaatgc cagctcgttg
    16201 cccttctgca gttctccggc agatgccagc atgcgttttc tgccattttc caattcgaaa
    16261 agcgaatact tgggcagttt gataatgagg tctttcttca cctctttgta acctggtttg
    16321 tataattttg gttcagttaa ttaggaaaat tacataatgg ctccaaaaaa aaattatcga
    16381 ccttttacca aaaaaaataa aatttatcga ccttgacagg aaaaagttac aaatcaaaag
    16441 aaagaatatc gtctggcatc caatatttta cctttagcct cgaggaagtc gatcggattc
    16501 ttctcgaacg aggacctttc cattatggta attccaagca gttccttgac gcttttcagt
    16561 ttcttgctct tgcccttctc caccttggcc actaccagta cgctgtatgc cacggtaggg
    16621 ctatcaaagc ctccgtattt cttcggatcc caatccttct tcctcgcaat cagtttgtcg
    16681 gagttgcgct tagggagaat ggactccttg gagaaaccac cggtttgaac ctccgttttc
    16741 ttgactatgt tgacttgggg catgctgaga accttacgca cagtcgcaaa atccctgccc
    16801 ttatcccaga caatctcacc tgcaagaaac agcaaagaga gagttaagat ataagtgaac
    16861 aagaaagtct tgggatcgac atacatacaa tcaattgatc aaattcctag gattaaacat
    16921 atgaaatagg aaagacagac ctgtttcgcc atttgtctcg atcagaggcc tcttccggat
    16981 ttcaccattc gccagagtga tctctgtctt gaagaagttc atgatattcg agtaaaagaa
    17041 gtatttcgcg gtagccttgc cgatctcttg ttctgacttc gcgatcatct tcctaacgtc
    17101 gtacaccttg taatccccgt acacaaactc agactccagt ttcggatact tcttgatgag
    17161 agcagtgcca acaacggcgt tcaggtaggc gtcatgggca tgatggtagt tgttgatctc
    17221 gcgaaccttg taaaactgga agtccttccg gaagtcacta accagtttgc tcttgagggt
    17281 gatcacctgc gtaaccatca aaagtcaata tggattcatc acaaattaat caggaataga
    17341 aagatcgggt ggccttgaag ttgatcaaat aaaaagtttc gaactttagt ttgatatggt
    17401 aaaggaaagt tggaaacttt acctttacct ccctgataag tttgtcattc tcatcatact
    17461 tcgtattcat acgcgaatcg agtatctggg ccacatgttt cgtaatctga cgagtttcaa
    17521 ccaactggcg cttgatgaag ccggctttgt cgagctcaga cagtccgccc ctttctgcct
    17581 tcgtgagatt gtcgaatttc cgttgggtta tgagcttagc attcaaaagc tgcctccaat
    17641 agttcttcat cttcttcaca acctcctcgc tgggtacatt gtcggatttt ccgcggttct
    17701 tgtctgagcg cgtaaggacc tataacacgg gagaaaccaa aaagcaaact catttcacgc
    17761 caaatactac agatttctca aaaattaagt tataatccat atatcctgac aagaaattga
    17821 ttaaaacaca gttgctttac cttgttgtca atactatcgt ccttcaggaa agattgaggg
    17881 acaatgtgat ccacatcata gtcggagagt ctgttaatgt cgagctcttg gtcgacgtac
    17941 atatctctcc cattctggag gtaatagagg tacagcttct cgttctggag ttgagtattt
    18001 tcgactgggt gctctttcag aatctgactc ccaagctcct tgatgccttc ctctatccgc
    18061 ttcatgcgct cccgtgagtt cttctgccct ttctgagtcg tctggttttc cctcgccatc
    18121 tcgatgacga tgttctccgg cttatggcgg cccatgacct gtagcaataa ttaaaacaaa
    18181 aataccacaa acagttagaa ctgaggcaag aagaatgaat gcagagaaaa aaaatcttag
    18241 ctattcatgg aaatggaagt gcgacgaaca cctaaaatgc aaggagcata accaaatgca
    18301 gaacttacct tcaccagctc atccacaacc ttcactgtct gcagtatgcc cttctttata
    18361 gctggcgatc cagccaggtt ggctatgtgt tcgtgaaggc tatcgccctg acctgacact
    18421 tgggccttct ggatatcttc tttgaaggtc agtgagtcat cgtgaataag ctgcatgaag
    18481 ttccgattcg caaaaccgtc ggatttgaga aagtccagta tagtcttgcc ggattgcttg
    18541 tcgcggatgc cgttgatgag cttcctggag agccttcccc aacctaaaac atagaacaaa
    18601 catttgtctc atcacaaata tgcatcaagg aacactcaga agttagtcat atacccaaat
    18661 tcttgaaact agagaaacaa aaaaagaggg atcattgcgg aaaccttttg acctcttacc
    18721 tgtatatctc ctcctcttca actgcttcat gaccttgtca tcgaacagat gcgcataagt
    18781 cttaaggcgc tcctcgatca tttctctgtc ctcaaacagc gtgagagtca gcacgatgtc
    18841 ctcgaggatg tcctcgttct cctcgttgtc aaggaagtcc ttgtccttga tgattttcaa
    18901 caggtcatgg tatgtgccaa gagaggcatt gaacctatcc tcaacgccgc tgatttccac
    18961 agaatcaaag cattcgatct tcttaaagta gtcctcttta agctgtttca cagtgacctt
    19021 ccgattggtt ttgaacaaga gatcgacaat ggccttcttc tgctcacctg ttataacagt
    19081 aaaaattact ttagaaacaa aacaaaagtg tggtctttta ggaatgatta ttgatcagtt
    19141 catagtttaa aatcttgtgt ttttatcgta aaagtgtgga ccaggattta cctgacagga
    19201 aggccggttt gcgcatcccc tccgtcacat atttcacctt cgtcagctcg ttgtagaccg
    19261 tgaagtattc gtacaggaga ctatgcttgg gcaggacttt ctcatttggg agattcttat
    19321 cgaagttggt catcctttcg atgaaggact gtgcactcgc acccttgtcc acgacctctt
    19381 cgaagttcca aggcgtgata gtctcctcgg atttgcgtgt catccacgca aaccgcgagt
    19441 tcccgcgtgc gagtggacct aatgaattaa tagaaaaatc aggcaaatta gaaaagaaat
    19501 aattctcaaa agagaacaac tgaggtcaga aaatagcatt catcaactga aactaggtat
    19561 ttcttcatat gatactaacc tacatagtag ggaatgcgga acgtgaggat cttttcaatc
    19621 ttctctctgt tgtctttcag gaacggatag aagtcctctt gcctcctcaa gatagcatgc
    19681 agctcaccga ggtggatctg gtgcggtatg ctgccattgt cgaaggtacg ctgcttccga
    19741 agcaaatctt cgcggttcaa tttaacgagc agctcctctg taccgtccat cttttccaga
    19801 ataggcttga tgaatttgta gaattcctcc tgacttgcac ctaataatat catcagagga
    19861 aaactcaatt cttatatctt acagaaatga acaacaaaat acatgtagca gagaggaaaa
    19921 caatcataag taacaactta cctccatcaa tgtaccctgc gtagccattc ttcgattggt
    19981 caaagaaaat ctccttgtac ttctcgggga gttgctggcg aaccaaagcc ttcagaaggg
    20041 tcagatcctg gtgatgctcg tcgtacctct tgatcatgga ggcgctaagt ggcgctttgg
    20101 ttatctccgt gttgacccgc agaatgtcac tgagcaagat agcgtctgac agattcttgg
    20161 cagccaagaa caggtcggca tactgatcac ctacaaaagt ttaaaagact caattttaac
    20221 tctacagctt caaataaatt tccgcaaaga aattgatagg tttagacaac aaaagaagag
    20281 ttccaatttc aagagcacct atttgggcga gcagattgtc cagatcatca tcgtatgtat
    20341 cctttgacag ctgcagcttc gcgtcctcgg cgaggtcaaa gttgcttttg aagttagggg
    20401 tgaggcccag agacagtgca atcagattcc cgaacaagcc gttcttcttc tcgcctggca
    20461 attgagcgat cagattctca agccgtctgg acttcgaaag tctggcagac agtattgcct
    20521 tggcgtcgac acctgagatt gtttgcaaag aaaaatctca aagcatgtac aatctataaa
    20581 cttatgatgg aaaaagaaaa gaaaggtaac taaggaatgt tacctgatgc attgatgggg
    20641 ttctcctcga acagctgatt gtaggtttgg acgagctgaa tgaacagttt atccacatcg
    20701 gagttatccg ggttgaggtc cccttcgatc aagaaatggc cacgaaactt tatcatgtgc
    20761 gcgagagcca ggtatatgag ccgaaggtct gccttatcgg ttgagtccac cagcttcttg
    20821 cgaaggtggt atatagtcgg atacttttca tgataggcta cttcgtcaac aatattccca
    20881 aagatggggt gacgctcgtg tttcttatct tcctctacca ggaaggattc ctcgaggcga
    20941 tggaagaacg agtcgtcaac ctgcaaaatc aagtttattg atcatcaagt gagtttacat
    21001 gtagttaaat gctttcagta atatcccaat gaagcaggat atgctttcag tacaaaatac
    21061 ataagcatac tatatcataa aaatccttac cttcgccatc tcgttgctaa agatctcttg
    21121 gagataacag attctgttct tcctgcgagt gtaccttctc ctcgcagtac gtttaagcct
    21181 tgtcgcctcc gcggtttctc cagagtcaaa gagcagagct ccgatcaagt tcttcttaat
    21241 gctgtgccta tctgtgttgc ccagcacttt aaatttcttc gaaggtacct tgtattcatc
    21301 ggtgataacg gcccacccaa ctgagttcgt accaatatca agtccaatgc tgtacttctt
    21361 gtccattttc cagctgacct tccgcttctt cttcggtggg ctagaagcca ttgtatcgat
    21421 aattgtaaat gtaattgtaa tgttgtttgt tgtttgttgt tgttggtaat tgttgtaaaa
    21481 atgagctctt atactcgagc gtgtcctctc caaatgaaat gaacttcctt atatagagga
    21541 agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatgtcaca
    21601 tcaatccact tgctttgtag acgtggttgg aacctcttct ttttccacga tgctcctcgt
    21661 gggtgggggt ccatctttgg gaccactgtc ggcagagaga tcttgaatga tagcctttcc
    21721 tttatcgcaa tgatggcatt tgtaggagcc accttccttt tctactgtcc tttcgatgaa
    21781 gtgacagata gctgggcaat ggaatccgag gaggtttccc gaaattatcc tttgttgaaa
    21841 agtctcaata gccctttgat cttctgagac tgtatctttg acatttttgg agtagaccag
    21901 agtgtcgtgc tccaccatgt tatcacatca atccacttgc tttgtagacg tggttggaac
    21961 ctcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
    22021 agagagatct tgaatgatag cctttccttt atcgcaatga tggcatttgt aggagccacc
    22081 ttccttttct actgtccttt cgatgaagtg acagatagct gggcaatgga atccgaggag
    22141 gtttcccgaa attatccttt gttgaaaagt ctcaatagcc ctttgatctt ctgagactgt
    22201 atctttgaca tttttggagt agaccagagt gtcgtgctcc accatgttga cctccaagct
    22261 tgaattcttg cagagaccga gacgttgatc ggcacgtaag aggttccaac tttcaccata
    22321 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg
    22381 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
    22441 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
    22501 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
    22561 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
    22621 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
    22681 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
    22741 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
    22801 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
    22861 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
    22921 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
    22981 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcaggggg
    23041 ggcgtaaacg ccgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
    23101 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
    23161 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
    23221 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
    23281 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
    23341 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
    23401 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
    23461 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
    23521 gataaagtct cccgtgaact ttacccagtg gtgcatatcg gggatgaaag ctggcgcatg
    23581 atgaccaccg atatggccag tgtgccggtt tccgttatcg gggaagaagt ggctgatctc
    23641 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
    23701 tcaggctccc ttatacacag ccagtcggtc tcagcttctg caggcatgcg acgtcgagct
    23761 tagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga
    23821 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
    23881 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
    23941 ttcccctcgg gatcaaagta ctttaaagta ctttaaagta ctttaaagta ctttgatcca
    24001 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt gttctgaaaa
    24061 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct t
//
