LOCUS       sequence-314749-        4524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ethanol inducible system trans activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: lvl0 AlcR
FEATURES             Location/Qualifiers
     source          1..4524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      33..90
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(114..131)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(285..873)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(365..384)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1056..1150)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1171..1830)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1745..1764
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1992..2010
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2014..2057)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gacagctttt tgtaggcttt gagaccctgc agtccggcaa aaaagggcaa ggtgtcacca
       61 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc
      121 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      181 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      241 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc
      301 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      361 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      421 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      481 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      541 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      601 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      661 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      721 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      781 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
      841 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
      901 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
      961 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1021 tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa aaacgcccgg
     1081 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     1141 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta
     1201 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     1261 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     1321 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     1381 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     1441 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     1501 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     1561 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     1621 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     1681 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     1741 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     1801 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     1861 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     1921 tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt atcatgacat
     1981 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt
     2041 tcgctaagga tgatttctgg aattcggtct ctaatggcag atacgcgccg acgccagaat
     2101 catagctgcg atccctgtcg caagggcaag cgacgctgtg atgccccgga aaatagaaac
     2161 gaggccaatg aaaacggctg ggtttcgtgt tcaaattgca agcgttggaa caaggattgt
     2221 accttcaatt ggctctcatc ccaacgctcc aaggcaaaag gggctgcacc tagagcgaga
     2281 acaaagaaag ccaggaccgc aacaaccacc agtgaaccat caacttcagc tgcaacaatc
     2341 cctacaccgg aaagtgacaa tcacgatgcg cctccagtca taaactctca cgacgcgctc
     2401 ccgagctgga ctcaggggct actctcccac cccggcgacc ttttcgattt cagccactct
     2461 gctattcccg caaatgcaga agatgcggcc aacgtgcagt cagacgcacc ttttccgtgg
     2521 gatctagcca tccccggtga tttcagcatg ggccaacagc tcgagaaacc tctcagtccg
     2581 ctcagttttc aagcagtcct tcttccgccc catagcccga acacggatga cctcattcgc
     2641 gagctggaag agcagactac ggatccggac tcggttaccg atactaatag tgtacaacag
     2701 gtcgctcaag atggatcgct atggtctgat cggcagtcgc cgctactgcc tgagaacagt
     2761 ctgtgcatgg cctcagacag cacagcacgg cgatatgccc gttccacaat gacgaagaat
     2821 ctgatgcgaa tctaccacga tagtatggag aatgcactgt cctgctggct gacagagcac
     2881 aattgtccat actccgacca gatcagctac ctgccgccca agcagcgggc ggaatggggc
     2941 ccgaactggt caaacaggat gtgcatccgg gtgtgccggc tagatcgcgt atctacctca
     3001 ttacgcgggc gcgccctgag tgcggaagag gacaaagccg cagcccgagc cctgcatctg
     3061 gcgatcgtag cttttgcgtc gcaatggacg cagcatgcgc agaggggggc tgggctaaat
     3121 gttcctgcag acatagccgc cgatgagagg tccatccgga ggaacgcctg gaatgaagca
     3181 cgccatgcct tgcagcacac gacagggatt ccatcattcc gggttatatt tgcgaatatc
     3241 atcttttctc tcacgcagag tgtgctggat gatgatgagc agcacggtat gggtgcacgt
     3301 ctagacaagc tactcgaaaa tgacggtgcg cccgtgttcc tggaaaccgc gaaccgtcag
     3361 ctttatacat tccgacataa gtttgcacga atgcaacgcc gcggtaaggc tttcaacagg
     3421 ctcccgggag gatctgtcgc atcgacattc gccggtattt tcgagacacc gacgccgtcg
     3481 tctgaaagcc cacagcttga cccggttgtg gccagtgagg agcatcgcag tacattaagc
     3541 cttatgttct ggctagggat catgttcgat acactaagcg ctgcaatgta ccagcgacca
     3601 ctcgtggtgt cagatgagga tagccagata tcatcggcat ctccaccaag gcgcggcgct
     3661 gaaacgccga tcaacctaga ctgctgggag cccccgagac aggtcccgag caatcaagaa
     3721 aagagcgacg tatggggcga cctcttcctc cgcacctcgg actctctccc agatcacgaa
     3781 tcccacacac aaatctctca gccagcggct cgatggccct gcacctacga acaggccgcc
     3841 gccgctctct cctctgcaac gcccgtcaaa gtcctcctct accgccgcgt cacgcagctc
     3901 caaaccctcc tctatcgcgg cgccagccct gcccgccttg aagcggccat ccagagaacg
     3961 ctctacgttt ataatcactg gacagcgaag taccaaccat ttatgcagga ctgcgttgct
     4021 aaccacgagc tcctcccttc gcgcatccag tcttggtacg tcattctaga cggtcactgg
     4081 catctagccg cgatgttgct agcggacgtt ttggagagca tcgaccgcga ttcgtactct
     4141 gatatcaacc acatcgacct tgtaacaaag ctaaggctcg ataatgcact agcagttagt
     4201 gcccttgcgc gctcttcact ccgaggccag gagctggacc cgggcaaagc atctccgatg
     4261 tatcgccatt tccatgattc tctgaccgag gtggcattcc tggtagaacc gtggaccgtc
     4321 gttcttattc actcgtttgc caaagctgcg tatatcttgc tggactgttt agatctggac
     4381 ggccaaggaa atgcactagc ggggtacctg cagctgcggc aaaattgcaa ctactgcatt
     4441 cgggcgctgc aatttctggg caggaagtcg gatatggcgg cgctggttgc gaaggattta
     4501 gagagaggtt tgaatgggaa agtt
//
