LOCUS       sequence-314752-        2778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yellow fluorescent protein CTAG codon optimised for Arabidopsis 
            thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: lvl0 eYFP CTAG
FEATURES             Location/Qualifiers
     source          1..2778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      34..91
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(115..132)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(286..874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(366..385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1057..1151)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1172..1831)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1746..1765
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1993..2011
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2015..2058)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 tgagctgtat aaataagctt tgagaccctg cagtccggca aaaaagggca aggtgtcacc
       61 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
      121 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      181 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      241 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg
      301 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      361 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      421 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      481 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      541 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      601 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      661 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      721 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      781 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      841 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      901 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
      961 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1021 atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg
     1081 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     1141 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt
     1201 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     1261 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     1321 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     1381 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     1441 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     1501 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     1561 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     1621 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     1681 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     1741 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     1801 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     1861 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     1921 ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca
     1981 ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
     2041 ttcgctaagg atgatttctg gaattcggtc tctttcggtg tcaaaaggcg aagagctttt
     2101 tactggggta gtacccatcc tggttgaatt ggatggagat gtgaacggac ataaatttag
     2161 tgtctctggg gagggcgagg gagacgccac ttacgggaaa ttaacgctca aattcatatg
     2221 cactactggt aaactaccgg tcccctggcc aacgctagta accacatttg gatatggctt
     2281 gcaatgtttc gctcgttacc cagatcacat gaagcaacac gattttttca aaagtgcgat
     2341 gcctgaaggt tatgtgcaag agcgaactat atttttcaaa gacgacggga actataagac
     2401 tagggctgaa gtaaagtttg agggggatac gctcgtcaat cgtattgagc ttaagggcat
     2461 cgattttaag gaggatggga acattttagg ccataagttg gagtacaact ataattctca
     2521 caatgtgtac attatggcag acaaacaaaa gaacggaata aaagtcaact tcaaaatcag
     2581 acataacata gaagacgggt cagtacagct cgccgatcac taccaacaaa acacaccgat
     2641 tggcgatggc cctgtgctac ttcctgataa ccattacctc tcttatcaaa gtgctctctc
     2701 aaaagacccc aacgagaaga gagatcacat ggttctcctg gagtttgtga ctgcggctgg
     2761 catcacttta ggaatgga
//
