LOCUS       sequence-314755-        8325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BRET biosensor for detection of Rho-GTP in mammalian expression 
            vector pcDNA3.1(+).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, 
            Garcia-Marcos M
  TITLE     Revealing the Activity of Trimeric G-proteins in Live Cells with a 
            Versatile Biosensor Design.
  JOURNAL   Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020.
  PUBMED    32634377
REFERENCE   2  (bases 1 to 8325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020."
COMMENT     Sequence Label: pcDNA3.1-Rho*-BERKY3
FEATURES             Location/Qualifiers
     source          1..8325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             742..1254
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             1279..1299
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2020..2271
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             2752..3003
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             3481..3732
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             4035..4064
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4099..4116)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4105..4329
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4375..4803
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4462..4481)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4672..4693
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4812..4832)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4817..5146
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4997..5132
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5059..5078
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5194..5241
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5260..5634
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5764..5885
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5801..5820)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5855..5874
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5934..5950)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5934..5950)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5947..5969)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5958..5974
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5982..5988,5989..6006,6007..6012))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6027..6048
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6165..6182)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6336..6924)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6416..6435)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7095..7886,7887..7955))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7718..7737
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7956..8060)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8135..8154)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcatgg gatgtataaa atcaaaaggg aaagacagca agcttggatc aggcggtagc
      721 gggggatcag gcggtagcgg gatggtcttc acactcgaag atttcgttgg ggactggcga
      781 cagacagccg gctacaacct ggaccaagtc cttgaacagg gaggtgtgtc cagtttgttt
      841 cagaatctcg gggtgtccgt aactccgatc caaaggattg tcctgagcgg tgaaaatggg
      901 ctgaagatcg acatccatgt catcatcccg tatgaaggtc tgagcggcga ccaaatgggc
      961 cagatcgaaa aaatttttaa ggtggtgtac cctgtggatg atcatcactt taaggtgatc
     1021 ctgcactatg gcacactggt aatcgacggg gttacgccga acatgatcga ctatttcgga
     1081 cggccgtatg aaggcatcgc cgtgttcgac ggcaaaaaga tcactgtaac agggaccctg
     1141 tggaacggca acaaaattat cgacgagcgc ctgatcaacc ccgacggctc cctgctgttc
     1201 cgagtaacca tcaacggagt gaccggctgg cggctgtgcg aacgcattct ggcggaattc
     1261 ggatctggtg gttcaggcga aaacctgtat tttcagggcg gaagcggaga agaggaggaa
     1321 aagaaaaaac aacaggagga ggaggcggag aggctgagga ggatacaaga ggaaatggaa
     1381 aaagaaagaa agcggcgaga ggaagatgag cagcgacgcc gcaaggagga ggaagagaga
     1441 aggatgaaat tggagatgga ggcaaaacgc aaacaagaag aggaagaacg gaagaaacgg
     1501 gaggatgatg aaaagcggaa gaaaaaggga tctggagggt cagggggcgc gcccatggtt
     1561 tcaaagggag aagagttgtt cacaggggtt gtaccgattc ttgtagaact tgacggagat
     1621 gtcaacgggc acaagtttag tgtaagtgga gaaggcgagg gcgatgcgac ctatggaaaa
     1681 ctgaccctta aatttatttg tactacgggt aaattgcccg ttccctggcc gacattggta
     1741 actacattcg gatatggttt gatgtgcttt gcaaggtatc cggatcacat gaagcagcat
     1801 gatttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1861 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1921 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1981 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     2041 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     2101 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     2161 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     2221 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcgcgccc
     2281 ggaggaatgg tttcaaaggg agaagagttg ttcacagggg ttgtaccgat tcttgtagaa
     2341 cttgacggag atgtcaacgg gcacaagttt agtgtaagtg gagaaggcga gggcgatgcg
     2401 acctatggaa aactgaccct taaatttatt tgtactacgg gtaaattgcc cgttccctgg
     2461 ccgacattgg taactacatt cggatatggt ttgatgtgct ttgcaaggta tccggatcac
     2521 atgaagcagc atgatttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     2581 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     2641 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2701 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     2761 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     2821 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     2881 aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     2941 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     3001 aagggatccg gaggaatggt ttcaaaggga gaagagttgt tcacaggggt tgtaccgatt
     3061 cttgtagaac ttgacggaga tgtcaacggg cacaagttta gtgtaagtgg agaaggcgag
     3121 ggcgatgcga cctatggaaa actgaccctt aaatttattt gtactacggg taaattgccc
     3181 gttccctggc cgacattggt aactacattc ggatatggtt tgatgtgctt tgcaaggtat
     3241 ccggatcaca tgaagcagca tgatttcttc aagtccgcca tgcccgaagg ctacgtccag
     3301 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     3361 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     3421 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     3481 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     3541 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     3601 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
     3661 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3721 gagctgtaca agggatccgg tggcaccggt ggatcaggct cttctcgagg aatcctggag
     3781 gacctcaata tgctctacat ccggcagatg gcactcagcc tggaggacac agagctgcag
     3841 aggaaactag atcatgagat ccggatgagg gatggggcct gcaagctgct ggcagcctgc
     3901 tcccagcgag aacaggctct ggaagccacc aagagcctgc tggtgtgcaa cagccgtatt
     3961 ctcagctaca tgggtgagct gcagcggcga aaggaggccc aggtgctgga gaagacaggc
     4021 tagctcgagg tggagaacaa aaacttattt ctgaagaaga tctgtgatct agagggcccg
     4081 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     4141 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     4201 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     4261 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     4321 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     4381 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4441 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4501 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4561 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     4621 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4681 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4741 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4801 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4861 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4921 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4981 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5041 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     5101 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     5161 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     5221 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     5281 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     5341 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     5401 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     5461 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     5521 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     5581 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     5641 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     5701 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     5761 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5821 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5881 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     5941 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6001 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6061 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6121 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6181 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6241 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6301 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6361 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6421 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6481 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6541 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6601 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6661 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6721 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     6781 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6841 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6901 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6961 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7021 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7081 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7141 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7201 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7261 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7321 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7381 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7441 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7501 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7561 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7621 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7681 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7741 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7801 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7861 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7921 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7981 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8041 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     8101 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     8161 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     8221 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     8281 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtt
//
