LOCUS       sequence-314759-        1673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast display plasmid intended for expression of N-terminal fusions 
            with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent 
            green-yellow fluorescent protein for detection of expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Zahradnik J, Dey D, Marciano S, Schreiber G
  TITLE     An enhanced yeast display platform demonstrates the binding 
            plasticity under various selection pressures
  JOURNAL   bioRxiv Preprint 2020
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            Preprint 2020"
COMMENT     Sequence Label: pJYDN
FEATURES             Location/Qualifiers
     source          1..1673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        182..623
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    182..299
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     594..617
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             1080..1091
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1092..1118
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1185..1214
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 acgccagcaa cgcggcactt tgtaactgag ctgtcatttt gctggcccag ctggtcgacc
       61 gcggctaagc agtactggta ccgttgtgga aatgtaaaga gccccattat cttagcctaa
      121 aaaaaccttc tctttggaac tttcagtaat acgcttaact gctcattgct atattgaagt
      181 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
      241 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      301 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      361 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      421 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      481 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      541 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      601 ctctatactt taacgtcaag gagaaaaaac cccggatcga attccctact tcatacattt
      661 tcaattaaga tgaggttccc atctattttc accgctgttg tttttgctgc ttcttctgct
      721 ttggctgctc cagctaatgg tcatatggaa gcataataat taaggcgcct tatctcgagg
      781 ggggcggatc ctctggtgga ggtggttcgg gcggaggtgg taacggatct aatggttctg
      841 gcggtagctc tgcacaggaa ctgacaacta tatgcgagca aatcccctca ccaactttag
      901 aatcgacgcc gtactctttg tcaacgacta ctattttggc caacgggaag gcaatgcaag
      961 gagtttttga atattacaaa tcagtaacgt ttgtcagtaa ttgcggttct cacccctcaa
     1021 caactagcaa aggcagcccc ataaacacac agtatgtttt taaggacaat agctcgacga
     1081 ttgaaggtag atacccatac gacgttccag actacgctct gcaggctagt ggtggaggag
     1141 gctctggtgg aggtggtagc ggaggcggag ggtcggctag ccatgaacag aagttgatct
     1201 ctgaagaaga tttgatgtta gaaaaatttg ttggcacctg gaagatcgaa tcctctgaaa
     1261 attttggtga atacttgaag gctatcggtg ccccaaaaga attggctgat gctggtgatg
     1321 ctactactcc agtcttgtac atttctcaaa aggatggtga taagatgacc gtcaagattg
     1381 aaaacggtcc accaactttt ttggataccc aagtttcttt caagttgggt gaagaattcg
     1441 acgaatttcc atccgataga agaaagggtg ttaagtccgt tgttaacttg tctggtgaga
     1501 agttggttta cgttcaaaag tgggatggta aagaaaccac ttacgtcaga gaaatcaagg
     1561 acggtaaatt ggttgttact ttgaccatgg gtgatgttgt tgctgttaga tcttatagaa
     1621 gggcctctga ataatagaga tctgataaca acagtgtaga tgtaacaaaa tcg
//
