LOCUS       sequence-314761-        1622 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Positive control plasmid carrying the eUnaG2 bilirubin dependent 
            fluorescent protein fused to the C-terminus of Aga2p protein. This 
            plasmid serves as an expression control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Zahradnik J, Dey D, Marciano S, Schreiber G
  TITLE     An enhanced yeast display platform demonstrates the binding 
            plasticity under various selection pressures
  JOURNAL   bioRxiv Preprint 2020
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            Preprint 2020"
COMMENT     Sequence Label: pJYDNp
FEATURES             Location/Qualifiers
     source          1..1622
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        182..623
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    182..299
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     594..617
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             1027..1038
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1039..1065
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1132..1161
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 acgccagcaa cgcggcactt tgtaactgag ctgtcatttt gctggcccag ctggtcgacc
       61 gcggctaagc agtactggta ccgttgtgga aatgtaaaga gccccattat cttagcctaa
      121 aaaaaccttc tctttggaac tttcagtaat acgcttaact gctcattgct atattgaagt
      181 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
      241 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      301 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      361 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      421 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      481 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      541 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      601 ctctatactt taacgtcaag gagaaaaaac cccggatcga attccctact tcatacattt
      661 tcaattaaga tgaggttccc atctattttc accgctgttg tttttgctgc ttcttctgct
      721 ttggctgctc cagctaatgg tcatatggaa gcatgcttaa ttaaggcgcc ttatctcgag
      781 gggggcggat ccgtttctgc acaggaactg acaactatat gcgagcaaat cccctcacca
      841 actttagaat cgacgccgta ctcattgtca acgactacta ttttggccaa cgggaaggca
      901 atgcaaggag tttttgaata ttacaaatca gtaacgtttg tcagtaattg cggttctcac
      961 ccctcaacaa ctagcaaagg cagccccata aacacacagt atgtttttaa ggacaatagc
     1021 tcgacgattg aaggtagata cccatacgac gttccagact acgctctgca ggctagtggt
     1081 ggaggaggct ctggtggagg tggtagcgga ggcggagggt cggctagcca tgaacagaag
     1141 ttgatctctg aagaagattt gatgttagaa aaatttgttg gcacctggaa gatcgaatcc
     1201 tctgaaaatt ttggtgaata cttgaaggct atcggtgccc caaaagaatt ggctgatgct
     1261 ggtgatgcta ctactccagt cttgtacatt tctcaaaagg atggtgataa gatgaccgtc
     1321 aagattgaaa acggtccacc aacttttttg gatacccaag tttctttcaa gttgggtgaa
     1381 gaattcgacg aatttccatc cgatagaaga aagggtgtta agtccgttgt taacttgtct
     1441 ggtgagaagt tggtttacgt tcaaaagtgg gatggtaaag aaaccactta cgtcagagaa
     1501 atcaaggacg gtaaattggt tgttactttg accatgggtg atgttgttgc tgttagatct
     1561 tatagaaggg cctctgaata atagagatct gataacaaca gtgtagatgt aacaaaatcg
     1621 ac
//
