LOCUS       sequence-314763-        1696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast display plasmid intended for expression of N-terminal fusions 
            with Aga2p yeast agglutinin. The plasmid is carrying the eUnaG2 
            fluorescent protein and long 2G linker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Zahradnik J, Dey D, Marciano S, Schreiber G
  TITLE     An enhanced yeast display platform demonstrates the binding 
            plasticity under various selection pressures
  JOURNAL   bioRxiv Preprint 2020
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            Preprint 2020"
COMMENT     Sequence Label: pJYDNg
FEATURES             Location/Qualifiers
     source          1..1696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        182..623
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    182..299
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     594..617
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             1101..1112
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1113..1139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1206..1235
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 acgccagcaa cgcggcactt tgtaactgag ctgtcatttt gctggcccag ctggtcgacc
       61 gcggctaagc agtactggta ccgttgtgga aatgtaaaga gccccattat cttagcctaa
      121 aaaaaccttc tctttggaac tttcagtaat acgcttaact gctcattgct atattgaagt
      181 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
      241 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      301 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      361 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      421 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      481 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      541 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      601 ctctatactt taacgtcaag gagaaaaaac cccggatcga attccctact tcatacattt
      661 tcaattaaga tgaggttccc atctattttc accgctgttg tttttgctgc ttcttctgct
      721 ttggctgctc cagctaatgg tcatatggaa gcataataat taaggcgcct tatctcgagg
      781 ggggcggatc cttctcccaa aagttggaca tcaacttgtt ggataacgtt gtcaactctt
      841 catatcatgg tgaaggtgtt tctggaggtt ctgcacagga actgacaact atatgcgagc
      901 aaatcccctc accaacttta gaatcgacgc cgtactcttt gtcaacgact actattttgg
      961 ccaacgggaa ggcaatgcaa ggagtttttg aatattacaa atcagtaacg tttgtcagta
     1021 attgcggttc tcacccctca acaactagca aaggcagccc cataaacaca cagtatgttt
     1081 ttaaggacaa tagctcgacg attgaaggta gatacccata cgacgttcca gactacgctc
     1141 tgcaggctag tggtggagga ggctctggtg gaggtggtag cggaggcgga gggtcggcta
     1201 gccatgaaca gaagttgatc tctgaagaag atttgatgtt agaaaaattt gttggcacct
     1261 ggaagatcga atcctctgaa aattttggtg aatacttgaa ggctatcggt gccccaaaag
     1321 aattggctga tgctggtgat gctactactc cagtcttgta catttctcaa aaggatggtg
     1381 ataagatgac cgtcaagatt gaaaacggtc caccaacttt tttggatacc caagtttctt
     1441 tcaagttggg tgaagaattc gacgaatttc catccgatag aagaaagggt gttaagtccg
     1501 ttgttaactt gtctggtgag aagttggttt acgttcaaaa gtgggatggt aaagaaacca
     1561 cttacgtcag agaaatcaag gacggtaaat tggttgttac tttgaccatg ggtgatgttg
     1621 ttgctgttag atcttataga agggcctctg aataatagag atctgataac aacagtgtag
     1681 atgtaacaaa atcgac
//
