LOCUS       sequence-31478-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TPMT
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             810..827
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(880..898)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gaatattttg tttactttag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggttcc gcgtggtagt actgaggtac agaaaaacca agtactaact
      121 ctggaagaat ggcaagacaa gtgggtgaac ggcaagactg cttttcatca ggaacaagga
      181 catcagctat taaagaagca tttagatact ttccttaaag gcaagagtgg actgagggta
      241 ttttttcctc tttgcggaaa agcggttgag atgaaatggt ttgcagaccg gggacacagt
      301 gtagttggtg tggaaatcag tgaacttggg atacaagaat tttttacaga gcagaatctt
      361 tcttactcag aagaaccaat caccgaaatt cctggaacca aagtatttaa gagttcttcg
      421 gggaacattt cattgtactg ttgcagtatt tttgatcttc ccaggacaaa tattggcaaa
      481 tttgacatga tttgggatag aggagcatta gttgccatta atccaggtga tcgcaaatgc
      541 tatgcagata caatgttttc cctcctggga aagaagtttc agtatctcct gtgtgttctt
      601 tcttatgatc caactaaaca tccaggtcca ccattttatg ttccacatgc tgaaattgaa
      661 aggttgtttg gtaaaatatg caatatacgt tgtcttgaga aggttgatgc ttttgaagaa
      721 cgacataaaa gttggggaat tgactgtctt tttgaaaagt tatatctact tacagaaaag
      781 tgatgacgaa gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg
      841 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      901 a
//
