LOCUS       sequence-314796-       13171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a concatemeric construct of a alpha-4:beta-2 (2:3) nAChR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13171)
  AUTHORS   Prevost MS, Bouchenaki H, Barilone N, Gielen M, Corringer PJ
  TITLE     Concatemers to re-investigate the role of alpha5 in alpha4beta2 
            nicotinic receptors.
  JOURNAL   Cell Mol Life Sci. 2020 May 29. pii: 10.1007/s00018-020-03558-z. 
            doi: 10.1007/s00018-020-03558-z.
  PUBMED    32472188
REFERENCE   2  (bases 1 to 13171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2020 May 29. pii: 10.1007/s00018-020-03558-z. doi: 
            10.1007/s00018-020-03558-z."
COMMENT     Sequence Label: pRK5-3(beta-2)
FEATURES             Location/Qualifiers
     source          1..13171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      955..964
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      9176..9185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    9189..9323
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9226..9245)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9280..9299
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        9392..9749
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9392..9583
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9415..9435)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9600..9735
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9769..9786)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9769..9785)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9778..9800)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10085..10104)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(10169..10360)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     10590..10611
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10765..10784)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10944..10962)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11030..11134
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11135..11203,11204..11995)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11353..11372)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12166..12754
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12655..12674
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12908..12925
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13042..13063
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13078..13083,13084..13101,13102..13108)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13116..13132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13121..13143
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13140..13156
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13140..13156
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tgcagaagct cagaataaac gctcaacttt ggcagatcat cgatgccacc
      961 atggagctag ggggccccgg agcgccgcgg ctgctgccgc cgctgctgct gcttctgggg
     1021 accggcctcc tgcgcgccag cagccatgtg gagacccggg cccacgccga ggagcggctc
     1081 ctgaagaaac tcttctccgg ttacaacaag tggtcccgac ccgtggccaa catctcggac
     1141 gtggtcctcg tccgcttcgg cctgtccatc gctcagctca ttgacgtgga tgagaagaac
     1201 cagatgatga ccacgaacgt atgggtgaag caggagtggc acgactacaa gctgcgctgg
     1261 gacccagctg actatgagaa tgtcacctcc atccgcatcc cctccgagct catctggcgg
     1321 ccggacatcg tcctctacaa caatgctgac ggggacttcg cggtcaccca cctgaccaag
     1381 gcccacctgt tccatgacgg gcgggtgcag tggactcccc cggccattta caagagctcc
     1441 tgcagcatcg acgtcacctt cttccccttc gaccagcaga actgcaccat gaaattcggc
     1501 tcctggacct acgacaaggc caagatcgac ctggtgaaca tgcacagccg cgtggaccag
     1561 ctggacttct gggagagtgg cgagtgggtc atcgtggatg ccgtgggcac ctacaacacc
     1621 aggaagtacg agtgctgtgc cgagatctac ccggacatca cctatgcctt cgtcatccgg
     1681 cggctgccgc tcttctacac catcaacctc atcatcccct gcctgctcat ctcctgcctc
     1741 accgtgctgg tcttctacct gccctccgag tgtggcgaga agatcacgct gtgcatctcc
     1801 gtgctgctgt cgctcaccgt cttcctgctg ctcatcaccg agatcatccc gtccacctca
     1861 ctggtcatcc cactcatcgg cgagtacctg ctgttcacca tgatcttcgt caccctgtcc
     1921 atcgtcatca cggtcttcgt gctcaacgtg caccaccgct cgccacgcac gcacaccatg
     1981 cccacctggg tacgcagggt cttcctggac atcgtgccac gcctgctcct catgaagcgg
     2041 ccgtccgtgg tcaaggacaa ttgccggcgg ctcatcgagt ccatgcataa gatggccagt
     2101 gccccgcgct tctggcccga gccagaaggg gagccccctg ccacgagcgg cacccagagc
     2161 ctgcacccgc cctcaccgtc cttctgtgtc cccctggatg tgccggctga gcctgggcct
     2221 tcctgcaagt caccctccga ccagctccct cctcagcagc ccctggaagc tgagaaagcc
     2281 agcccccacc cctcgcctgg accctgccgc ccgccccacg gcacccaggc accagggctg
     2341 gccaaagcca ggtccctcag cgtccagcac atgtccagcc ctggcgaagc ggtggaaggc
     2401 ggcgtccggt gccggtctcg gagcatccag tactgtgttc cccgagacga tgccgccccc
     2461 gaggcagatg gccaggctgc cggcgccctg gcctctcgca acacccactc ggctgagctc
     2521 ccacccccag accagccctc tccgtgcaaa tgcacatgca agaaggagcc ctcttcggtg
     2581 tccccgagcg ccacggtcaa gacccgcagc accaaagcgc cgcccccgca cctgcccctg
     2641 tcgccggccc tgacccgggc ggtggagggc gtccagtaca ttgcagacca cctgaaggcc
     2701 gaagacacag acttctcggt gaaggaggac tggaagtacg tggccatggt catcgaccgc
     2761 atcttcctct ggatgttcat catcgtctgc ctgctgggga cggtgggcct cttcctgccg
     2821 ccctggctgg ctggcatgat ccagcaacag cagcaacaac agcaacagca gcaacagcag
     2881 caacaacagc aacagcagca aaccggtacg gatacagagg agcggctggt ggagcatctc
     2941 ctggatcctt cccgctacaa caagctcatc cgcccagcca ccaatggctc tgagctggtg
     3001 acagtacagc ttatggtgtc actggcccag ctcatcagtg tgcatgagcg ggagcagatc
     3061 atgaccacca atgtctggct gacccaggag tgggaagatt atcgcctcac ctggaagcct
     3121 gaagagtttg acaacatgaa gaaagttcgg ctcccttcca aacacatctg gctcccagat
     3181 gtggtcctgt acaacaatgc tgacggcatg tacgaggtgt ccttctattc caatgccgtg
     3241 gtctcctatg atggcagcat cttctggctg ccgcctgcca tctacaagag cgcatgcaag
     3301 attgaagtaa agcacttccc atttgaccag cagaactgca ccatgaagtt ccgttcgtgg
     3361 acctacgacc gcacagagat cgacttggtg ctgaagagtg aggtggccag cctggacgac
     3421 ttcacaccta gtggtgagtg ggacatcgtg gcgctgccgg gccggcgcaa cgagaacccc
     3481 gacgactcta cgtacgtgga catcacgtat gacttcatca ttcgccgcaa gccgctcttc
     3541 tacaccatca acctcatcat cccctgtgtg ctcatcacct cgctggccat ccttgtcttc
     3601 tacctgccat ccgactgtgg cgagaagatg acgttgtgca tctcagtgct gctggcgctc
     3661 acggtcttcc tgctgctcat ctccaagatc gtgcctccca cctccctcga cgtgccgctc
     3721 gtcggcaagt acctcatgtt caccatggtg cttgtcacct tctccatcgt caccagcgtg
     3781 tgcgtgctca acgtgcacca ccgctcgccc accacgcaca ccatggcgcc ctgggtgaag
     3841 gtcgtcttcc tggagaagct gcccgcgctg ctcttcatgc agcagccacg ccatcattgc
     3901 gcccgtcagc gcctgcgcct gcggcgacgc cagcgtgagc gcgagggcgc tggagccctc
     3961 ttcttccgcg aagccccagg ggccgactcc tgcacgtgct tcgtcaaccg cgcgtcggtg
     4021 caggggttgg ccggggcctt cggggctgag cctgcaccag tggcgggccc cgggcgctca
     4081 ggggagccgt gtggctgtgg cctccgggag gcggtggacg gcgtgcgctt catcgcagac
     4141 cacatgcgga gcgaggacga tgaccagagc gtgagtgagg actggaagta cgtcgccatg
     4201 gtgatcgacc gcctcttcct ctggatcttt gtctttgtct gtgtctttgg caccatcggc
     4261 atgttcctgc agcctctctt ccagaactac accaccacca ccttcctcca ctcagaccac
     4321 tcagccccca gctccaagca gcaacagcag caacaacagc aacagcagca acagcagcaa
     4381 caagtcgacc atgtggagac ccgggcccac gccgaggagc ggctcctgaa gaaactcttc
     4441 tccggttaca acaagtggtc ccgacccgtg gccaacatct cggacgtggt cctcgtccgc
     4501 ttcggcctgt ccatcgctca gctcattgac gtggatgaga agaaccagat gatgaccacg
     4561 aacgtatggg tgaagcagga gtggcacgac tacaagctgc gctgggaccc agctgactat
     4621 gagaatgtca cctccatccg catcccctcc gagctcatct ggcggccgga catcgtcctc
     4681 tacaacaatg ctgacgggga cttcgcggtc acccacctga ccaaggccca cctgttccat
     4741 gacgggcggg tgcagtggac tcccccggcc atttacaaga gctcctgcag catcgacgtc
     4801 accttcttcc ccttcgacca gcagaactgc accatgaaat tcggctcctg gacctacgac
     4861 aaggccaaga tcgacctggt gaacatgcac agccgcgtgg accagctgga cttctgggag
     4921 agtggcgagt gggtcatcgt ggatgccgtg ggcacctaca acaccaggaa gtacgagtgc
     4981 tgtgccgaga tctacccgga catcacctat gccttcgtca tccggcggct gccgctcttc
     5041 tacaccatca acctcatcat cccctgcctg ctcatctcct gcctcaccgt gctggtcttc
     5101 tacctgccct ccgagtgtgg cgagaagatc acgctgtgca tctccgtgct gctgtcgctc
     5161 accgtcttcc tgctgctcat caccgagatc atcccgtcca cctcactggt catcccactc
     5221 atcggcgagt acctgctgtt caccatgatc ttcgtcaccc tgtccatcgt catcacggtc
     5281 ttcgtgctca acgtgcacca ccgctcgcca cgcacgcaca ccatgcccac ctgggtacgc
     5341 agggtcttcc tggacatcgt gccacgcctg ctcctcatga agcggccgtc cgtggtcaag
     5401 gacaattgcc ggcggctcat cgagtccatg cataagatgg ccagtgcccc gcgcttctgg
     5461 cccgagccag aaggggagcc ccctgccacg agcggcaccc agagcctgca cccgccctca
     5521 ccgtccttct gtgtccccct ggatgtgccg gctgagcctg ggccttcctg caagtcaccc
     5581 tccgaccagc tccctcctca gcagcccctg gaagctgaga aagccagccc ccacccctcg
     5641 cctggaccct gccgcccgcc ccacggcacc caggcaccag ggctggccaa agccaggtcc
     5701 ctcagcgtcc agcacatgtc cagccctggc gaagcggtgg aaggcggcgt ccggtgccgg
     5761 tctcggagca tccagtactg tgttccccga gacgatgccg cccccgaggc agatggccag
     5821 gctgccggcg ccctggcctc tcgcaacacc cactcggctg agctcccacc cccagaccag
     5881 ccctctccgt gcaaatgcac atgcaagaag gagccctctt cggtgtcccc gagcgccacg
     5941 gtcaagaccc gcagcaccaa agcgccgccc ccgcacctgc ccctgtcgcc ggccctgacc
     6001 cgggcggtgg agggcgtcca gtacattgca gaccacctga aggccgaaga cacagacttc
     6061 tcggtgaagg aggactggaa gtacgtggcc atggtcatcg accgcatctt cctctggatg
     6121 ttcatcatcg tctgcctgct ggggacggtg ggcctcttcc tgccgccctg gctggctggc
     6181 atgatccagc aacagcagca acaacagcaa cagcagcaac agcagcaaca acagcaacag
     6241 cagcaagcta gcacggatac agaggagcgg ctggtggagc atctcctgga tccttcccgc
     6301 tacaacaagc tcatccgccc agccaccaat ggctctgagc tggtgacagt acagcttatg
     6361 gtgtcactgg cccagctcat cagtgtgcat gagcgggagc agatcatgac caccaatgtc
     6421 tggctgaccc aggagtggga agattatcgc ctcacctgga agcctgaaga gtttgacaac
     6481 atgaagaaag ttcggctccc ttccaaacac atctggctcc cagatgtggt cctgtacaac
     6541 aatgctgacg gcatgtacga ggtgtccttc tattccaatg ccgtggtctc ctatgatggc
     6601 agcatcttct ggctgccgcc tgccatctac aagagcgcat gcaagattga agtaaagcac
     6661 ttcccatttg accagcagaa ctgcaccatg aagttccgtt cgtggaccta cgaccgcaca
     6721 gagatcgact tggtgctgaa gagtgaggtg gccagcctgg acgacttcac acctagtggt
     6781 gagtgggaca tcgtggcgct gccgggccgg cgcaacgaga accccgacga ctctacgtac
     6841 gtggacatca cgtatgactt catcattcgc cgcaagccgc tcttctacac catcaacctc
     6901 atcatcccct gtgtgctcat cacctcgctg gccatccttg tcttctacct gccatccgac
     6961 tgtggcgaga agatgacgtt gtgcatctca gtgctgctgg cgctcacggt cttcctgctg
     7021 ctcatctcca agatcgtgcc tcccacctcc ctcgacgtgc cgctcgtcgg caagtacctc
     7081 atgttcacca tggtgcttgt caccttctcc atcgtcacca gcgtgtgcgt gctcaacgtg
     7141 caccaccgct cgcccaccac gcacaccatg gcgccctggg tgaaggtcgt cttcctggag
     7201 aagctgcccg cgctgctctt catgcagcag ccacgccatc attgcgcccg tcagcgcctg
     7261 cgcctgcggc gacgccagcg tgagcgcgag ggcgctggag ccctcttctt ccgcgaagcc
     7321 ccaggggccg actcctgcac gtgcttcgtc aaccgcgcgt cggtgcaggg gttggccggg
     7381 gccttcgggg ctgagcctgc accagtggcg ggccccgggc gctcagggga gccgtgtggc
     7441 tgtggcctcc gggaggcggt ggacggcgtg cgcttcatcg cagaccacat gcggagcgag
     7501 gacgatgacc agagcgtgag tgaggactgg aagtacgtcg ccatggtgat cgaccgcctc
     7561 ttcctctgga tctttgtctt tgtctgtgtc tttggcacca tcggcatgtt cctgcagcct
     7621 ctcttccaga actacaccac caccaccttc ctccactcag accactcagc ccccagctcc
     7681 aagcagcaac agcagcaaca acagcaacag cagcaacagc agcaacaact cgagacggat
     7741 acagaggagc ggctggtgga gcatctcctg gatccttccc gctacaacaa gctcatccgc
     7801 ccagccacca atggctctga gctggtgaca gtacagctta tggtgtcact ggcccagctc
     7861 atcagtgtgc atgagcggga gcagatcatg accaccaatg tctggctgac ccaggagtgg
     7921 gaagattatc gcctcacctg gaagcctgaa gagtttgaca acatgaagaa agttcggctc
     7981 ccttccaaac acatctggct cccagatgtg gtcctgtaca acaatgctga cggcatgtac
     8041 gaggtgtcct tctattccaa tgccgtggtc tcctatgatg gcagcatctt ctggctgccg
     8101 cctgccatct acaagagcgc atgcaagatt gaagtaaagc acttcccatt tgaccagcag
     8161 aactgcacca tgaagttccg ttcgtggacc tacgaccgca cagagatcga cttggtgctg
     8221 aagagtgagg tggccagcct ggacgacttc acacctagtg gtgagtggga catcgtggcg
     8281 ctgccgggcc ggcgcaacga gaaccccgac gactctacgt acgtggacat cacgtatgac
     8341 ttcatcattc gccgcaagcc gctcttctac accatcaacc tcatcatccc ctgtgtgctc
     8401 atcacctcgc tggccatcct tgtcttctac ctgccatccg actgtggcga gaagatgacg
     8461 ttgtgcatct cagtgctgct ggcgctcacg gtcttcctgc tgctcatctc caagatcgtg
     8521 cctcccacct ccctcgacgt gccgctcgtc ggcaagtacc tcatgttcac catggtgctt
     8581 gtcaccttct ccatcgtcac cagcgtgtgc gtgctcaacg tgcaccaccg ctcgcccacc
     8641 acgcacacca tggcgccctg ggtgaaggtc gtcttcctgg agaagctgcc cgcgctgctc
     8701 ttcatgcagc agccacgcca tcattgcgcc cgtcagcgcc tgcgcctgcg gcgacgccag
     8761 cgtgagcgcg agggcgctgg agccctcttc ttccgcgaag ccccaggggc cgactcctgc
     8821 acgtgcttcg tcaaccgcgc gtcggtgcag gggttggccg gggccttcgg ggctgagcct
     8881 gcaccagtgg cgggccccgg gcgctcaggg gagccgtgtg gctgtggcct ccgggaggcg
     8941 gtggacggcg tgcgcttcat cgcagaccac atgcggagcg aggacgatga ccagagcgtg
     9001 agtgaggact ggaagtacgt cgccatggtg atcgaccgcc tcttcctctg gatctttgtc
     9061 tttgtctgtg tctttggcac catcggcatg ttcctgcagc ctctcttcca gaactacacc
     9121 accaccacct tcctccactc agaccactca gcccccagct ccaagtagaa gcttggccgc
     9181 catggcccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9241 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9301 atgtatctta tcatgtctgg atcgggaatt aattcggcgc agcaccatgg cctgaaataa
     9361 cctctgaaag aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg
     9421 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9481 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9541 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     9601 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     9661 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     9721 gcttttttgg aggcctaggc ttttgcaaaa agctgttaac agcttggcac tggccgtcgt
     9781 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     9841 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     9901 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     9961 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
    10021 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    10081 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggcct tacagacaag
    10141 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
    10201 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct
    10261 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa
    10321 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg
    10381 gatttctgtt catgggggta atgataccga tgaaacggcc ggctttcccc gtcaagctct
    10441 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    10501 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
    10561 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    10621 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    10681 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
    10741 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
    10801 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
    10861 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
    10921 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
    10981 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
    11041 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    11101 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    11161 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    11221 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    11281 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    11341 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
    11401 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    11461 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    11521 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    11581 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    11641 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    11701 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    11761 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    11821 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    11881 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    11941 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    12001 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    12061 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    12121 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    12181 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    12241 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    12301 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    12361 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    12421 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    12481 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    12541 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    12601 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    12661 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    12721 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    12781 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    12841 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    12901 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
    12961 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
    13021 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
    13081 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
    13141 aggaaacagc tatgacatga ttacgaatta a
//
