LOCUS       sequence-31480-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MAK3
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..97
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             631..648
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(701..719)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      715..762
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ccctctgaat attttgttta ctttaagaag gagatatacc atgggcagca gccatcatca
       61 tcatcatcac agcagcggcc tggttccgcg tggtagtaaa ggtagccgga tcgagctggg
      121 agatgtgaca ccacacaata ttaaacagtt gaaaagattg aatcaggtca tctttccagt
      181 cagctacaat gacaagttct acaaggatgt gctggaggtt ggcgagctag caaaacttgc
      241 ctatttcaat gatattgctg taggtgcagt atgctgtagg gtggatcatt cacagaatca
      301 gaagagactt tacatcatga cactaggatg tctggcacct taccgaaggc taggaatagg
      361 aactaaaatg ttaaatcatg tcttaaacat ctgtgaaaaa gatggtactt ttgacaacat
      421 ttatctgcat gtccagatca gcaatgagtc ggcaattgac ttctacagga agtttggctt
      481 tgagattatt gagacaaaga agaactacta taagaggata gagcccgcag atgctcatgt
      541 gctgcagaaa aacctcaaag ttccttctgg tcagaatgca gatgtgcaaa agacagacaa
      601 ctgatgacga agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
      661 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      721 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      781 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      841 ttacgcgcag cgtgaccgct acactttgcc agcgccctag cgcccgctcc ctttcg
//
