LOCUS       sequence-314800-       13537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a concatemeric construct of a alpha-4:beta-2 (3:2) nAChR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13537)
  AUTHORS   Prevost MS, Bouchenaki H, Barilone N, Gielen M, Corringer PJ
  TITLE     Concatemers to re-investigate the role of alpha5 in alpha4beta2 
            nicotinic receptors.
  JOURNAL   Cell Mol Life Sci. 2020 May 29. pii: 10.1007/s00018-020-03558-z. 
            doi: 10.1007/s00018-020-03558-z.
  PUBMED    32472188
REFERENCE   2  (bases 1 to 13537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2020 May 29. pii: 10.1007/s00018-020-03558-z. doi: 
            10.1007/s00018-020-03558-z."
COMMENT     Sequence Label: pRK5-3(alpha-4)
FEATURES             Location/Qualifiers
     source          1..13537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      955..964
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      9542..9551
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    9555..9689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9592..9611)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9646..9665
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        9758..10115
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9758..9949
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9781..9801)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9966..10101
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10135..10152)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10135..10151)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10144..10166)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10451..10470)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(10535..10726)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     10956..10977
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11131..11150)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11310..11328)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11396..11500
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11501..11569,11570..12361)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11719..11738)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12532..13120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13021..13040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13274..13291
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13408..13429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13444..13449,13450..13467,13468..13474)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13482..13498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13487..13509
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13506..13522
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13506..13522
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tgcagaagct cagaataaac gctcaacttt ggcagatcat cgatgccacc
      961 atggagctag ggggccccgg agcgccgcgg ctgctgccgc cgctgctgct gcttctgggg
     1021 accggcctcc tgcgcgccag cagccatgtg gagacccggg cccacgccga ggagcggctc
     1081 ctgaagaaac tcttctccgg ttacaacaag tggtcccgac ccgtggccaa catctcggac
     1141 gtggtcctcg tccgcttcgg cctgtccatc gctcagctca ttgacgtgga tgagaagaac
     1201 cagatgatga ccacgaacgt atgggtgaag caggagtggc acgactacaa gctgcgctgg
     1261 gacccagctg actatgagaa tgtcacctcc atccgcatcc cctccgagct catctggcgg
     1321 ccggacatcg tcctctacaa caatgctgac ggggacttcg cggtcaccca cctgaccaag
     1381 gcccacctgt tccatgacgg gcgggtgcag tggactcccc cggccattta caagagctcc
     1441 tgcagcatcg acgtcacctt cttccccttc gaccagcaga actgcaccat gaaattcggc
     1501 tcctggacct acgacaaggc caagatcgac ctggtgaaca tgcacagccg cgtggaccag
     1561 ctggacttct gggagagtgg cgagtgggtc atcgtggatg ccgtgggcac ctacaacacc
     1621 aggaagtacg agtgctgtgc cgagatctac ccggacatca cctatgcctt cgtcatccgg
     1681 cggctgccgc tcttctacac catcaacctc atcatcccct gcctgctcat ctcctgcctc
     1741 accgtgctgg tcttctacct gccctccgag tgtggcgaga agatcacgct gtgcatctcc
     1801 gtgctgctgt cgctcaccgt cttcctgctg ctcatcaccg agatcatccc gtccacctca
     1861 ctggtcatcc cactcatcgg cgagtacctg ctgttcacca tgatcttcgt caccctgtcc
     1921 atcgtcatca cggtcttcgt gctcaacgtg caccaccgct cgccacgcac gcacaccatg
     1981 cccacctggg tacgcagggt cttcctggac atcgtgccac gcctgctcct catgaagcgg
     2041 ccgtccgtgg tcaaggacaa ttgccggcgg ctcatcgagt ccatgcataa gatggccagt
     2101 gccccgcgct tctggcccga gccagaaggg gagccccctg ccacgagcgg cacccagagc
     2161 ctgcacccgc cctcaccgtc cttctgtgtc cccctggatg tgccggctga gcctgggcct
     2221 tcctgcaagt caccctccga ccagctccct cctcagcagc ccctggaagc tgagaaagcc
     2281 agcccccacc cctcgcctgg accctgccgc ccgccccacg gcacccaggc accagggctg
     2341 gccaaagcca ggtccctcag cgtccagcac atgtccagcc ctggcgaagc ggtggaaggc
     2401 ggcgtccggt gccggtctcg gagcatccag tactgtgttc cccgagacga tgccgccccc
     2461 gaggcagatg gccaggctgc cggcgccctg gcctctcgca acacccactc ggctgagctc
     2521 ccacccccag accagccctc tccgtgcaaa tgcacatgca agaaggagcc ctcttcggtg
     2581 tccccgagcg ccacggtcaa gacccgcagc accaaagcgc cgcccccgca cctgcccctg
     2641 tcgccggccc tgacccgggc ggtggagggc gtccagtaca ttgcagacca cctgaaggcc
     2701 gaagacacag acttctcggt gaaggaggac tggaagtacg tggccatggt catcgaccgc
     2761 atcttcctct ggatgttcat catcgtctgc ctgctgggga cggtgggcct cttcctgccg
     2821 ccctggctgg ctggcatgat ccagcaacag cagcaacaac agcaacagca gcaacagcag
     2881 caacaacagc aacagcagca aaccggtacg gatacagagg agcggctggt ggagcatctc
     2941 ctggatcctt cccgctacaa caagctcatc cgcccagcca ccaatggctc tgagctggtg
     3001 acagtacagc ttatggtgtc actggcccag ctcatcagtg tgcatgagcg ggagcagatc
     3061 atgaccacca atgtctggct gacccaggag tgggaagatt atcgcctcac ctggaagcct
     3121 gaagagtttg acaacatgaa gaaagttcgg ctcccttcca aacacatctg gctcccagat
     3181 gtggtcctgt acaacaatgc tgacggcatg tacgaggtgt ccttctattc caatgccgtg
     3241 gtctcctatg atggcagcat cttctggctg ccgcctgcca tctacaagag cgcatgcaag
     3301 attgaagtaa agcacttccc atttgaccag cagaactgca ccatgaagtt ccgttcgtgg
     3361 acctacgacc gcacagagat cgacttggtg ctgaagagtg aggtggccag cctggacgac
     3421 ttcacaccta gtggtgagtg ggacatcgtg gcgctgccgg gccggcgcaa cgagaacccc
     3481 gacgactcta cgtacgtgga catcacgtat gacttcatca ttcgccgcaa gccgctcttc
     3541 tacaccatca acctcatcat cccctgtgtg ctcatcacct cgctggccat ccttgtcttc
     3601 tacctgccat ccgactgtgg cgagaagatg acgttgtgca tctcagtgct gctggcgctc
     3661 acggtcttcc tgctgctcat ctccaagatc gtgcctccca cctccctcga cgtgccgctc
     3721 gtcggcaagt acctcatgtt caccatggtg cttgtcacct tctccatcgt caccagcgtg
     3781 tgcgtgctca acgtgcacca ccgctcgccc accacgcaca ccatggcgcc ctgggtgaag
     3841 gtcgtcttcc tggagaagct gcccgcgctg ctcttcatgc agcagccacg ccatcattgc
     3901 gcccgtcagc gcctgcgcct gcggcgacgc cagcgtgagc gcgagggcgc tggagccctc
     3961 ttcttccgcg aagccccagg ggccgactcc tgcacgtgct tcgtcaaccg cgcgtcggtg
     4021 caggggttgg ccggggcctt cggggctgag cctgcaccag tggcgggccc cgggcgctca
     4081 ggggagccgt gtggctgtgg cctccgggag gcggtggacg gcgtgcgctt catcgcagac
     4141 cacatgcgga gcgaggacga tgaccagagc gtgagtgagg actggaagta cgtcgccatg
     4201 gtgatcgacc gcctcttcct ctggatcttt gtctttgtct gtgtctttgg caccatcggc
     4261 atgttcctgc agcctctctt ccagaactac accaccacca ccttcctcca ctcagaccac
     4321 tcagccccca gctccaagca gcaacagcag caacaacagc aacagcagca acagcagcaa
     4381 caagtcgacc atgtggagac ccgggcccac gccgaggagc ggctcctgaa gaaactcttc
     4441 tccggttaca acaagtggtc ccgacccgtg gccaacatct cggacgtggt cctcgtccgc
     4501 ttcggcctgt ccatcgctca gctcattgac gtggatgaga agaaccagat gatgaccacg
     4561 aacgtatggg tgaagcagga gtggcacgac tacaagctgc gctgggaccc agctgactat
     4621 gagaatgtca cctccatccg catcccctcc gagctcatct ggcggccgga catcgtcctc
     4681 tacaacaatg ctgacgggga cttcgcggtc acccacctga ccaaggccca cctgttccat
     4741 gacgggcggg tgcagtggac tcccccggcc atttacaaga gctcctgcag catcgacgtc
     4801 accttcttcc ccttcgacca gcagaactgc accatgaaat tcggctcctg gacctacgac
     4861 aaggccaaga tcgacctggt gaacatgcac agccgcgtgg accagctgga cttctgggag
     4921 agtggcgagt gggtcatcgt ggatgccgtg ggcacctaca acaccaggaa gtacgagtgc
     4981 tgtgccgaga tctacccgga catcacctat gccttcgtca tccggcggct gccgctcttc
     5041 tacaccatca acctcatcat cccctgcctg ctcatctcct gcctcaccgt gctggtcttc
     5101 tacctgccct ccgagtgtgg cgagaagatc acgctgtgca tctccgtgct gctgtcgctc
     5161 accgtcttcc tgctgctcat caccgagatc atcccgtcca cctcactggt catcccactc
     5221 atcggcgagt acctgctgtt caccatgatc ttcgtcaccc tgtccatcgt catcacggtc
     5281 ttcgtgctca acgtgcacca ccgctcgcca cgcacgcaca ccatgcccac ctgggtacgc
     5341 agggtcttcc tggacatcgt gccacgcctg ctcctcatga agcggccgtc cgtggtcaag
     5401 gacaattgcc ggcggctcat cgagtccatg cataagatgg ccagtgcccc gcgcttctgg
     5461 cccgagccag aaggggagcc ccctgccacg agcggcaccc agagcctgca cccgccctca
     5521 ccgtccttct gtgtccccct ggatgtgccg gctgagcctg ggccttcctg caagtcaccc
     5581 tccgaccagc tccctcctca gcagcccctg gaagctgaga aagccagccc ccacccctcg
     5641 cctggaccct gccgcccgcc ccacggcacc caggcaccag ggctggccaa agccaggtcc
     5701 ctcagcgtcc agcacatgtc cagccctggc gaagcggtgg aaggcggcgt ccggtgccgg
     5761 tctcggagca tccagtactg tgttccccga gacgatgccg cccccgaggc agatggccag
     5821 gctgccggcg ccctggcctc tcgcaacacc cactcggctg agctcccacc cccagaccag
     5881 ccctctccgt gcaaatgcac atgcaagaag gagccctctt cggtgtcccc gagcgccacg
     5941 gtcaagaccc gcagcaccaa agcgccgccc ccgcacctgc ccctgtcgcc ggccctgacc
     6001 cgggcggtgg agggcgtcca gtacattgca gaccacctga aggccgaaga cacagacttc
     6061 tcggtgaagg aggactggaa gtacgtggcc atggtcatcg accgcatctt cctctggatg
     6121 ttcatcatcg tctgcctgct ggggacggtg ggcctcttcc tgccgccctg gctggctggc
     6181 atgatccagc aacagcagca acaacagcaa cagcagcaac agcagcaaca acagcaacag
     6241 cagcaagcta gcacggatac agaggagcgg ctggtggagc atctcctgga tccttcccgc
     6301 tacaacaagc tcatccgccc agccaccaat ggctctgagc tggtgacagt acagcttatg
     6361 gtgtcactgg cccagctcat cagtgtgcat gagcgggagc agatcatgac caccaatgtc
     6421 tggctgaccc aggagtggga agattatcgc ctcacctgga agcctgaaga gtttgacaac
     6481 atgaagaaag ttcggctccc ttccaaacac atctggctcc cagatgtggt cctgtacaac
     6541 aatgctgacg gcatgtacga ggtgtccttc tattccaatg ccgtggtctc ctatgatggc
     6601 agcatcttct ggctgccgcc tgccatctac aagagcgcat gcaagattga agtaaagcac
     6661 ttcccatttg accagcagaa ctgcaccatg aagttccgtt cgtggaccta cgaccgcaca
     6721 gagatcgact tggtgctgaa gagtgaggtg gccagcctgg acgacttcac acctagtggt
     6781 gagtgggaca tcgtggcgct gccgggccgg cgcaacgaga accccgacga ctctacgtac
     6841 gtggacatca cgtatgactt catcattcgc cgcaagccgc tcttctacac catcaacctc
     6901 atcatcccct gtgtgctcat cacctcgctg gccatccttg tcttctacct gccatccgac
     6961 tgtggcgaga agatgacgtt gtgcatctca gtgctgctgg cgctcacggt cttcctgctg
     7021 ctcatctcca agatcgtgcc tcccacctcc ctcgacgtgc cgctcgtcgg caagtacctc
     7081 atgttcacca tggtgcttgt caccttctcc atcgtcacca gcgtgtgcgt gctcaacgtg
     7141 caccaccgct cgcccaccac gcacaccatg gcgccctggg tgaaggtcgt cttcctggag
     7201 aagctgcccg cgctgctctt catgcagcag ccacgccatc attgcgcccg tcagcgcctg
     7261 cgcctgcggc gacgccagcg tgagcgcgag ggcgctggag ccctcttctt ccgcgaagcc
     7321 ccaggggccg actcctgcac gtgcttcgtc aaccgcgcgt cggtgcaggg gttggccggg
     7381 gccttcgggg ctgagcctgc accagtggcg ggccccgggc gctcagggga gccgtgtggc
     7441 tgtggcctcc gggaggcggt ggacggcgtg cgcttcatcg cagaccacat gcggagcgag
     7501 gacgatgacc agagcgtgag tgaggactgg aagtacgtcg ccatggtgat cgaccgcctc
     7561 ttcctctgga tctttgtctt tgtctgtgtc tttggcacca tcggcatgtt cctgcagcct
     7621 ctcttccaga actacaccac caccaccttc ctccactcag accactcagc ccccagctcc
     7681 aagcagcaac agcagcaaca acagcaacag cagcaacagc agcaacaact cgagcatgtg
     7741 gagacccggg cccacgccga ggagcggctc ctgaagaaac tcttctccgg ttacaacaag
     7801 tggtcccgac ccgtggccaa catctcggac gtggtcctcg tccgcttcgg cctgtccatc
     7861 gctcagctca ttgacgtgga tgagaagaac cagatgatga ccacgaacgt atgggtgaag
     7921 caggagtggc acgactacaa gctgcgctgg gacccagctg actatgagaa tgtcacctcc
     7981 atccgcatcc cctccgagct catctggcgg ccggacatcg tcctctacaa caatgctgac
     8041 ggggacttcg cggtcaccca cctgaccaag gcccacctgt tccatgacgg gcgggtgcag
     8101 tggactcccc cggccattta caagagctcc tgcagcatcg acgtcacctt cttccccttc
     8161 gaccagcaga actgcaccat gaaattcggc tcctggacct acgacaaggc caagatcgac
     8221 ctggtgaaca tgcacagccg cgtggaccag ctggacttct gggagagtgg cgagtgggtc
     8281 atcgtggatg ccgtgggcac ctacaacacc aggaagtacg agtgctgtgc cgagatctac
     8341 ccggacatca cctatgcctt cgtcatccgg cggctgccgc tcttctacac catcaacctc
     8401 atcatcccct gcctgctcat ctcctgcctc accgtgctgg tcttctacct gccctccgag
     8461 tgtggcgaga agatcacgct gtgcatctcc gtgctgctgt cgctcaccgt cttcctgctg
     8521 ctcatcaccg agatcatccc gtccacctca ctggtcatcc cactcatcgg cgagtacctg
     8581 ctgttcacca tgatcttcgt caccctgtcc atcgtcatca cggtcttcgt gctcaacgtg
     8641 caccaccgct cgccacgcac gcacaccatg cccacctggg tacgcagggt cttcctggac
     8701 atcgtgccac gcctgctcct catgaagcgg ccgtccgtgg tcaaggacaa ttgccggcgg
     8761 ctcatcgagt ccatgcataa gatggccagt gccccgcgct tctggcccga gccagaaggg
     8821 gagccccctg ccacgagcgg cacccagagc ctgcacccgc cctcaccgtc cttctgtgtc
     8881 cccctggatg tgccggctga gcctgggcct tcctgcaagt caccctccga ccagctccct
     8941 cctcagcagc ccctggaagc tgagaaagcc agcccccacc cctcgcctgg accctgccgc
     9001 ccgccccacg gcacccaggc accagggctg gccaaagcca ggtccctcag cgtccagcac
     9061 atgtccagcc ctggcgaagc ggtggaaggc ggcgtccggt gccggtctcg gagcatccag
     9121 tactgtgttc cccgagacga tgccgccccc gaggcagatg gccaggctgc cggcgccctg
     9181 gcctctcgca acacccactc ggctgagctc ccacccccag accagccctc tccgtgcaaa
     9241 tgcacatgca agaaggagcc ctcttcggtg tccccgagcg ccacggtcaa gacccgcagc
     9301 accaaagcgc cgcccccgca cctgcccctg tcgccggccc tgacccgggc ggtggagggc
     9361 gtccagtaca ttgcagacca cctgaaggcc gaagacacag acttctcggt gaaggaggac
     9421 tggaagtacg tggccatggt catcgaccgc atcttcctct ggatgttcat catcgtctgc
     9481 ctgctgggga cggtgggcct cttcctgccg ccctggctgg ctggcatgat ctagaagctt
     9541 ggccgccatg gcccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9601 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9661 tcatcaatgt atcttatcat gtctggatcg ggaattaatt cggcgcagca ccatggcctg
     9721 aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt
     9781 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9841 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     9901 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     9961 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
    10021 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
    10081 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct gttaacagct tggcactggc
    10141 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
    10201 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
    10261 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
    10321 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
    10381 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
    10441 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggccttaca
    10501 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
    10561 aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt
    10621 ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc
    10681 tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt
    10741 aagggggatt tctgttcatg ggggtaatga taccgatgaa acggccggct ttccccgtca
    10801 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
    10861 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
    10921 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    10981 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
    11041 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
    11101 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
    11161 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
    11221 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
    11281 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
    11341 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
    11401 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    11461 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    11521 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    11581 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    11641 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    11701 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
    11761 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    11821 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    11881 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    11941 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    12001 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    12061 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    12121 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    12181 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    12241 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    12301 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    12361 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    12421 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    12481 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    12541 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    12601 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    12661 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    12721 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    12781 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    12841 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    12901 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    12961 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    13021 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    13081 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    13141 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    13201 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    13261 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
    13321 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    13381 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    13441 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    13501 tcacacagga aacagctatg acatgattac gaattaa
//
