LOCUS       sequence-314807-       10031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This insert, when expressed in the same cell with other split Cre 
            component, forms Cre recombinase through protein trans-splicing 
            using split intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Luan H, Kuzin A, Odenwald WF, White BH
  TITLE     Cre-assisted fine-mapping of neural circuits using orthogonal split 
            inteins.
  JOURNAL   Elife. 2020 Apr 14;9. pii: 53041. doi: 10.7554/eLife.53041.
  PUBMED    32286225
REFERENCE   2  (bases 1 to 10031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-04-14"; volume: "9. pii: 53041. doi"; pages: " 
            10.7554/eLife.53041"
COMMENT     Sequence Label: HJP-194-IVS-Syn21-CreA-gp41-1N-WPREUw
FEATURES             Location/Qualifiers
     source          1..10031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     39..57
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    85..209
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(234..239,240..257,258..264)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             318..977
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(384..403)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1319..1624
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1503..1522
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1665..1789)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          1977..2043
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     misc_feature    2680..3268
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2733..2753)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3151..3162)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3529..3545)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3529..3545)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(4556..5170,5241..5372,5576..5891,5953..6607,
                     6682..6955,7357..7428))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     7880..7898
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    8123..8192
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(8279..8867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8359..8378)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9038..9829,9830..9898))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9661..9680
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9899..10003)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
       61 ttcgtcttca agaattcgtt tatcacaagt ttgtacaaaa aagctgaacg agaaacgtaa
      121 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
      181 tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac
      241 tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag
      301 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
      361 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
      421 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
      481 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
      541 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
      601 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
      661 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
      721 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
      781 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
      841 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
      901 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
      961 ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa cagtatgcgt
     1021 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
     1081 caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca
     1141 gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat
     1201 gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag
     1261 gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat
     1321 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
     1381 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
     1441 gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
     1501 gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc
     1561 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
     1621 ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat
     1681 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
     1741 tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtg ataaacggcc
     1801 ggccgagctc gcccggggat cgagcgcagc ggtataaaag ggcgcggggt ggctgagagc
     1861 atcagttgtg aatgaatgtt cgagccgagc agacgtgccg ctgccttcgt taatatcctt
     1921 tgaataagcc aactttgaat cacaagacgc ataccaaacg gtaccgctag aaaaaggtag
     1981 gttcaaccac tgatgcctag gcacaccgaa acgactaacc ctaattctta tcctttactt
     2041 cagggctagc aacttaaaaa aaaaaatcaa aatgagtaac ttgctcacgg tacaccaaaa
     2101 tttgccggcc ctgcctgtgg acgccacgtc cgacgaggtc cgaaagaatc tgatggacat
     2161 gttccgtgac aggcaggcct ttagcgagca cacctggaag atgctgctct cggtgtgcag
     2221 gtcctgggcc gcttggtgca agctgaacaa ccgaaagtgg tttcccgcgg agcccgagga
     2281 cgtccgcgat tatctgctgt acctgcaggc tcgcggactg gccgtcaaaa caatacagca
     2341 acatttgggt cagctgaaca tgctccacag gcgcagcgga ctgccccggc catccgattg
     2401 cctggatctg aagacccagg tgcagacccc ccagggcatg aaggagatca gcaacatcca
     2461 ggtgggcgat ctggtgctga gcaacaccgg ctacaacgag gtgctgaacg tgttccccaa
     2521 gagcaagaag aagagctaca agatcaccct ggaggatggc aaggagatca tctgcagcga
     2581 ggagcacctg ttccccaccc agaccggcga gatgaacatc agcggcggcc tgaaggaggg
     2641 catgtgcctg tacgtgaagg agtaaaccgg tgtttaaaca atcaacctct ggattacaaa
     2701 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2761 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     2821 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     2881 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     2941 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3001 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3061 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     3121 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3181 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3241 cggatctccc tttgggccgc ctccccgcct gaagcttatc gataccgtcg actaaagcca
     3301 aatagaaaat tattcagttc ctggcttaag tttttaaaag tgatattatt tatttggttg
     3361 taaccaacca aaagaatgta aataactaat acataattat gttagtttta agttagcaac
     3421 aaattgattt tagctatatt agctacttgg ttaataaata gaatatattt atttaaagat
     3481 aattgcgttt ttattgtcag ggagtgagtt tgcttaaaaa ctcgtttaga tccactagtt
     3541 ctagagcggc cgctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct
     3601 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg
     3661 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt
     3721 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggag
     3781 gcgcgcccta gttccagtga aatccaagca ttttctaaat taaatgtatt cttattatta
     3841 tagttgttat ttttgatata tataaacaac actattatgc ccaccatttt tttgagatgc
     3901 atctacacaa ggaacaaaca ctggatgtca ctttcagttc aaattgtaac gctaatcact
     3961 ccgaacaggt cacaaaaaat taccttaaaa agtcataata ttaaattaga ataaatatag
     4021 ctgtgaggga aatatataca aatatattgg agcaaataaa ttgtacatac aaatatttat
     4081 tactaatttc tattgagacg aaatgaacca ctcggaacca tttgagcgaa ccgaatcgcg
     4141 cggaactaac gacagtcgct ccaaggtcgt cgaacaaaag gtgaatgtgt tgcggagagc
     4201 gggtgggaga cagcgaaaga gcaactacga aacgtggtgt ggtggaggtg aattatgaag
     4261 agggcgcgcg atttgaaaag tatgtatata aaaaatatat cccggtgttt tatgtagcga
     4321 taaacgagtt tttgatgtaa ggtatgcagg tgtgtaagtc ttttggttag aagacaaatc
     4381 caaagtctac ttgtggggat gttcgaaggg gaaatacttg tattctatag gtcatatctt
     4441 gtttttattg gcacaaatat aattacatta gctttttgag ggggcaataa acagtaaaca
     4501 cgatggtaat aatggtaaaa aaaaaaaaca agcagttatt tcggatatat gtcggctact
     4561 ccttgcgtcg ggcccgaagt cttagagcca gatatgcgag cacccggaag ctcacgatga
     4621 gaatggccag acccacgtag tccagcggca gatcggcggc ggagaagtta agcgtctcca
     4681 ggatgacctt gcccgaactg gggcacgtgg tgttcgacga tgtgcagcta atttcgcccg
     4741 gctccacgtc cgcccattgg ttaatcagca gaccctcgtt ggcgtaacgg aaccatgaga
     4801 ggtacgacaa ccatttgagg tatactggca ccgagcccga gttcaagaag aagccgccaa
     4861 agagcaggaa tggtatgata accggcggac ccacagacag cgccatcgag gtcgaggagc
     4921 tggcgcagga tattagatat ccgaaggacg ttgacacatt ggccaccaga gtgaccagcg
     4981 ccaggcagtt gaagaagtgc agcactccgg cccgcagtcc gatcatcgga taggcaatcg
     5041 ccgtgaagac cagtggcact gtgagaaaaa gcggcaattc ggcaatcgtt ttgcccagaa
     5101 agtatgtgtc acagcgataa agtcgacttc gggcctccct cataaaaact ggcagctctg
     5161 aggtgaacac ctaaatcgaa tcgattcatt agaaagttag taaattattg aaatgcaaat
     5221 gtattctaaa catgacttac atttatcgtg gcaaagacgt tttgaaaggt catgttggtc
     5281 aggaagagga agatggctcc gttgatattc atcacaccca cttgcgtgag ttgttggccc
     5341 aaaaagatga ggccaatcaa gatggcaacc atctgcaaat taaaatgtta ctcgcatctc
     5401 attaatattc gcgagttaaa tgaaatttat ttatcttctg caaaactata aactatacat
     5461 ctcattgaaa aaaactaaga agggtgtgga atcaggcaat tctatctaaa atctagcgaa
     5521 tttgtttcca agaattgtaa gcgttatatc atttgtttcc actggaacca ctcaccgttg
     5581 tctgaataag tcgcactttt acgaggagtg gttccttgag caccgacagc caggatcgcc
     5641 acaggaccgc ccggaactgc atgaaccagg tggccttgta ggtgtaccca ttctccggct
     5701 gctccagtgg cttctccaga tttttggtgg ccaacaactg ctccatatcc cgggctactt
     5761 tgctaatggc aaaattgtcg catatcttgg cgatccgatc acgggactcg atctcccgtc
     5821 cgggcacaac ggccaacacc tgtacgtaaa agtccgccgg attgtagttg gtaggacact
     5881 gggcacccac gctggatagg agttgagatg ttatgtaata ctagataccc ttaataaaca
     5941 catcgaactc actaggaaaa gaagtcgacg gcttcgctgg gagtgcccaa gaaagctacc
     6001 ctgccctcgg ccatcagaag gatcttgtca aagagctcaa acagctcgga agacggctga
     6061 tgaatggtca ggatgacggt cttgcccttc tgcgacagct tcttcagcac ctggacgacg
     6121 ctgtgggcgg taaaggagtc cagtccggag gtgggctcat cgcagatcag aagcggcgga
     6181 tcggttagag cctcggaggc gaatgccaga cgcttccttt ctccgccgga cagacctttc
     6241 accctgccgg gcacaccgat gatcgtgtgc tgacatttgc tgagcgaaag ctcctggatc
     6301 acctgatcca cgcgggccac tcgctgccga taggtcagat gtcgtggcat ccgcaccatg
     6361 gcttggaaaa tcaggtgttc cctggccgtt agggagccga taaagaggtc atcctgctgg
     6421 acataggcgc acctggcctg catctccttg gcgtccacag gttggccatt gagcagtcgc
     6481 atcccggatg gcgatacttg gatgccctgc ggcgatcgaa aggcaagggc attcagcagg
     6541 gtcgtctttc cggcaccgga actgcccatc acggccaaaa gttcgcccgg ataggccacg
     6601 ccgcaaactg agtttcaaat tggtaattgg accctttatt aagatttcac acagatcagc
     6661 cgactgcgaa tagaaactca ccgttcttga gcaaatgttt cctgggcgcc ggtatgtgtc
     6721 gctcgttgca gaatagtccg cgtgtccggt tgaccagctg ccgccatccg gagcccggct
     6781 gattgaccgc cccaaagatg tccatattgt gccaggcata ggtgaggttc tcggctagtt
     6841 ggccgctccc tgaaccggag tcctccggcg gactgggtgg ccggagcgtg ccgtagtttt
     6901 tggcctgccc gaagccctgg ttaatgcagc tctgcgaagc cgctccgctg tcaccctgca
     6961 atgatagggg atctcaaata tcaactacta gcgttatgct catctaaccc cgaacaaaaa
     7021 gtaccccgaa gtatcctacg aagtaggttt atacttttat ttattttttg tgcatctagg
     7081 atcagcttaa aatatctggt tgttatattt tttgtaaaaa agaatatagt cgaaaatgaa
     7141 tgcctttaga tgtcttgatc atgatatgat ctcaaaaatt gtcttatata gcgagaacag
     7201 ctaccagaat aatctgtttc gtgtcactat ttgtttgtgc aattgcggtt tgggattttt
     7261 gtgggtcgca gttctcacgc cgcatacaat ttgatgttgc aatcgcagtt cctatagatc
     7321 aagtgaactt aagatgtatg cacatgtact actcacattg ttcagatgct cggcagatgg
     7381 gtgtttgctg cctccgcgaa ttaatagctc ctgatcctct tggcccattg ccgggatttt
     7441 tcacactttc ccctgcttac ccacccaaaa ccaatcacca ccccaatcac tcaaaaaaca
     7501 aacaaaaata agaagcgaga ggagttttgg cacagcactt tgtgtttaat tgatggcgta
     7561 aaccgcttgg agcttcgtca cgaaaccgct gacaaaatgc aactgaaggc ggacattgac
     7621 gctacgtaac gctacaaacg gtggcgaaag agatagcgga cgcagcggcg aaagagacgg
     7681 cgatatttct gtggacagag aaggaggcaa acagcgctga ctttgagtgg aatgtcattt
     7741 tgagtgagag gtaatcgaaa gaacctggta catcaaatac ccttggatcg aagtaaattt
     7801 aaaactgatc agataagttc aatgatatcc agtgcagtaa aaaaaaaaaa tgtttttttt
     7861 atctactttc cgcaaaaatg ggttttatta acttacatac atactaggcg cgcccatatg
     7921 ttcggcttgt cgacatgccc gccgtgaccg tcgagaaccc gctgacgctg ccccgcgtat
     7981 ccgcacccgc cgacgccgtc gcacgtcccg tgctcaccgt gaccaccgcg cccagcggtt
     8041 tcgagggcga gggcttcccg gtgcgccgcg cgttcgccgg gatcaactac cgccacctcg
     8101 acccgttcat catgatggac cagatgggtg aggtggagta cgcgcccggg gagcccaagg
     8161 gcacgccctg gcacccgcac cgcggcttcg agaccgtgac ctacatcgtc gacggtaaca
     8221 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8281 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8341 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8401 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8461 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8521 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8581 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8641 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8701 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8761 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8821 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8881 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8941 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9001 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9061 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9121 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9181 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9241 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9301 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9361 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9421 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9481 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9541 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9601 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9661 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9721 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9781 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9841 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9901 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9961 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10021 tgccacctga g
//
