LOCUS       sequence-314813-       18199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A bicistronic construct - FloxGal80 and UAS-mCD8GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18199)
  AUTHORS   Luan H, Kuzin A, Odenwald WF, White BH
  TITLE     Cre-assisted fine-mapping of neural circuits using orthogonal split 
            inteins.
  JOURNAL   Elife. 2020 Apr 14;9. pii: 53041. doi: 10.7554/eLife.53041.
  PUBMED    32286225
REFERENCE   2  (bases 1 to 18199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-04-14"; volume: "9. pii: 53041. doi"; pages: " 
            10.7554/eLife.53041"
COMMENT     Sequence Label: 
            HJP-224-actin5C^Syn21Gal80^IVS-Syn21myr-tdTomato-10UAS-mCD8GFP-p10w
FEATURES             Location/Qualifiers
     source          1..18199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..107
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        162..2671
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     protein_bind    complement(4439..4463)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    4478..4511
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          5933..5998
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6128..6148
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6420..6554
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    6561..6594
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          6606..6672
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             complement(join(10080..10694,10765..10896,11100..11415,
                     11477..12131,12206..12479,12881..12952))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     13404..13422
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    13462..13556
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    13590..13684
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        13715..13953
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     13900..13921
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             13983..14648
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
     CDS             14655..15368
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(14685..14713)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     15308..15328
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     intron          15455..15520
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             15650..15670
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    15942..16076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    16289..16358
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(16445..17033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16525..16544)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(17204..17995,17996..18064))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17827..17846
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18065..18169)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gcaggctccg cggccgcaat
      121 tctatattct aaaaacacaa atgatacttc taaaaaaaaa tcatgaatgg catcaactct
      181 gaatcaaatc tttgcagatg cacctacttc tcatttccac tgtcacatca tttttccaga
      241 tctcgctgcc tgttatgtgg cccacaaacc aagacacgtt ttatggccat taaagctggc
      301 tgatcgtcgc caaacaccaa atacataatg aatatgtaca cattcgagaa agaagcgatc
      361 aaagaagcgt cttcgggcgg agtaggagaa tgcggaggag aaggagaacg agctgatcta
      421 gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc aatttgaagc
      481 caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac cgttacccgg
      541 ggccattttc aaagactcgt cggcaagata agattgtgtc actcgctgtc tctcttcatt
      601 tgtcgaagaa tgctgaggaa tttcgcgatg acgtcggcga gtattttgaa gaatgagaat
      661 aatttgtatt tatacgaaaa tcagttagtg gaattttcta caaaaacatg ttatctatag
      721 ataattttgt tgcaaaatat gttgactatg acaaagattg tatgtatata cctttaatgt
      781 attctcattt tcttatgtat ttataatggc aatgatgata ctgatgatat tttaagatga
      841 tgccagacca aaaggcttga atttctgcgt cttttgccga acgcagtgca tgtgcaattg
      901 ttgttttttg gaatattcaa ttttcggact gtccgctttg atttcagttt cttggcttat
      961 tcaaaaagca aagtaaagcc aaaaaagcga gatggcaata ccaaatgcgg caaaacggta
     1021 gtggaaggaa aggggtgcgg ggcagcggaa ggaagggtgg ggcggggcgt ggcggggtct
     1081 gtggctgggc gcgacgtcac cgacgttgga gccactcctt tgaccatgtg tgcgtgtgtg
     1141 tattattcgt gtctcgccac tcgccggttg tttttttctt tttatgctgc gctctctcta
     1201 gcgccatctc gcttacgcat gctcaacgca ccgcatgttg ccgtttcctt ttatgcgtca
     1261 ttttggctcg aaataggcaa ttatttaaac aaagattagt caacgaaaac gctaaaataa
     1321 ataagtctac aatatggtta cttattgcca tgtgtgtgca gccaacgata gcaacaaaag
     1381 caacaacaca ggtggctttc cctctttcac tttttgtttg caagccgcgt gcgagcaaga
     1441 cggcacgacc ggcaaacgca attacgctga caaagagcag acgaagtttt ggcgaaaaac
     1501 atcaaggcgc ctgatacgaa tgcatttgca ataacaattg cgatatttaa tattgtttat
     1561 gaagctgttt gacttcaaaa cacacaaaaa aaaaaataaa acaaattatt tgaaagagaa
     1621 ttaggaatcg gacgcttatc gttagggtaa caacaagaaa tgcttactga gtcacagcct
     1681 ctggaaaact gccgcaagcc agagagagag agaaaaagag ggagagcagc ttagaccgca
     1741 tgtgcttgtg tgtgaggcgt ctctctcttc gtctctgttg cgcaaacgca tagactgcac
     1801 tgaaaaaatc gattacctat tttttatgaa tgaatatttg cactattact attcaaaact
     1861 attaagatag caatcacatt caatagccaa atactatacc acctgagcga tgcaacgaaa
     1921 tgatcaattt gagcaaaaat gctgcatatt taggacggca tcattataga aatgcttctt
     1981 gctgtgtact tttctctcgt ctggcagctg tttcgccgtt attgttaaaa ccggcttaag
     2041 ttaggtgtgt tttctacgac tagtgaatgc cctactagaa gatgtgtgtt gcacaaaatg
     2101 tccctggaat aaccaatttg aagtgcagat agcagtaaac gtaagctaat atgaatatta
     2161 tttaactgta atgttttaat atcgctggac attactaata aacccactat aaacacatgt
     2221 acatatgtat gttttggcat acaatgagta gttggggaaa aaatgtgtaa aagcaccgtg
     2281 accatcacag cataaagata accagctgaa gtatcgaata tgagtaaccc ccaaattgaa
     2341 tcacatgccg caactgatag gacccatgga agtacactct tcatggcgat atacaagaca
     2401 cacacaagca cgaacaccca gttgcggagg aaattctccg taaatgaaaa cccaatcggc
     2461 gaacaattca tacccatata tggtaaaagt tttgaacgcg acttgagagc ggagagcatt
     2521 gcggctgata aggttttagc gctaagcggg ctttataaaa cgggctgcgg gaccagtttt
     2581 catatcacta ccgtttgagt tcttgtgctg tgtggatact cctcccgaca caaagccgct
     2641 ccatcagcca gcagtcgtct aatccagaga caccaaaccg aaagacttaa tttatattta
     2701 tttaattaat tttaataaaa cacaccaaat gtaagtagct ttccccttcc caacaacaaa
     2761 acaccatcga accactccca ccaagaaaaa gcaataatcg agaaaagccg cggaaaatgt
     2821 gtgatttttt ttgtaaacaa aacatttttt tatgtgccag tgctgaaagt gatcaaaaaa
     2881 tactagccac gagctaaaga gttattgtat tgaccaaaac tccaaaaata cccaagtttg
     2941 gccctaaatt gtcaatcaaa ataccaatag gtcgaaagac atcaaaatta acaaaaccag
     3001 ggtttcaaat accataactc aagaatcagg attacaactg cagatttcag gatatataca
     3061 tacaaattat acgaaattat aaaaaccaaa gcaattcaat agccccaact caaatgttag
     3121 gatctaatat agtgtttaaa gccaagctcg ctgatgtggg cgtgtcacga tttcacccaa
     3181 agatatgcca aattacgaat tgcaaatcaa ttcgccaaca ctttcttttt ttcccacgcc
     3241 ctaaaacacc agatcatcca taaatgtaca tacatacagt atatgcatat tataatctgt
     3301 aaaactagat caggttcttg aaaatagtga cgtaggcagc cgttttggct gaagcagaaa
     3361 tttttgccgg tttttcaaag ttgtagttgc aaaaatggag aaaaccttcg agcattcgtt
     3421 catatacaca cactcacgcg caaaataacg agagagagtg tatgtgtgtg tgagagagcg
     3481 aaagccagac gacggtttgc ttttcgcctc gaaacatgac catatatggt cacaaaactt
     3541 ggccgccgca attcaacaca ccagcgctct ccttcgcacc catagcgacc atggcgcgga
     3601 gcgagcgaga tggcgagagc gagcgacgcc tatggcgacg tcgacgcagg cagcgattga
     3661 aaaacgcagt taactggcat tcaacattca ccagccactt tcagtcggtt tattccagtc
     3721 attcctttca aaccgtgcgg tcgcttagct cagcctcgcc acttgcgttt acagtagttt
     3781 tcacgccttg aatttgttaa atcgaacaaa aaggtaaagt ttaactagct ttgaaaagtt
     3841 tcgtggctct taattgttaa attttctaga gtgcgtttag tgtttttttt tttttttatt
     3901 ttgtaatgtt aatttcgggt tccaattcga gttttaggca gccgccattt taagggcgca
     3961 tacacacagg caactgtgct ctctttgcgg ctttcttttg caccggcatt cgttaagtgt
     4021 cgtctagaag cttctcccct cccttttcgg catattcgta ttgtggtttt aatttttcgg
     4081 ggcggggcct tctattttgt aactgttctt ttaatttctt attacaattc gatcgcaagt
     4141 gaaaatcagt tttcaatcgg aaaagtattt ttttatgaaa tttttttttg tccaagatta
     4201 aaattttgta ctaaaaaaac gtacattgca ttgagtgatt tttaattgta cacgaaaaac
     4261 aagttagttt gttatgacaa ttgtactttg gtagaccagc gcagtccaag gaaaccacgc
     4321 aaattctcag tttttttttt gccatttcta cattaccaaa taaggtaacc aaaaactaat
     4381 gggaaatccg cattctttcc attgcagctt acaggatcga tccccgggta ccgcgccgac
     4441 ccagctttct tgtacaaagt ggtgataaac ggccggcata acttcgtata atgtatgcta
     4501 tacgaagtta tcctgcagga acttaaaaaa aaaaatcaaa atggattaca acaaaaggag
     4561 tagtgtgagt acggtgccga atgctgctcc cattcgcgtg ggcttcgtgg gattgaacgc
     4621 ggctaagggt tgggccatta aaacgcatta tccagccata ctgcagctga gctcccagtt
     4681 ccaaataaca gcgctttatt cccccaagat cgagacgtcc attgcgacaa ttcagaggct
     4741 gaagttgtcc aacgccacag ctttcccaac actcgagagc ttcgcctcga gctccacgat
     4801 cgatatgatt gtgatcgcaa tccaggtggc ttcccactac gaggtggtaa tgcccctgct
     4861 cgagttttcg aagaacaatc ctaatctgaa gtatttgttt gtggaatggg cactggcctg
     4921 ctcgctcgat caggcggagt cgatctataa ggccgctgca gagcggggcg tgcaaacaat
     4981 aatcagtctg cagggacgaa agagcccgta cattttgcgg gcaaaagagc ttatctccca
     5041 gggctacatc ggcgatataa atagcatcga gattgcaggt aacggaggtt ggtacggtta
     5101 cgagcggccg gtgaaaagcc cgaaatacat ttacgagatc ggaaacggag ttgatctggt
     5161 gaccaccacg ttcggtcaca cgatagatat attgcagtac atgaccagtt cgtacttcag
     5221 ccgtatcaat gccatggtgt ttaacaacat tccagaacag gagctcattg atgagagggg
     5281 caatcgcctg ggccagcggg tcccaaagac tgtcccagat catctcttgt tccagggcac
     5341 gctgctcaac ggaaatgttc ctgtgtcctg ttcctttaag ggaggcaagc ctacgaaaaa
     5401 gttcaccaag aatctggtca tagatatcca tggtacaaag ggtgatctga agctggaggg
     5461 tgacgctgga tttgcggaaa tctcgaatct cgttctgtat tattcgggca cccgcgccaa
     5521 tgattttccc ctggccaatg gtcagcaggc acccttggac cccggctacg acgcaggcaa
     5581 ggagattatg gaggtgtatc acctccgaaa ctacaacgcg atcgttggta atatacaccg
     5641 gctctaccag agtatcagcg atttccactt caacaccaag aaaatccccg agcttccatc
     5701 gcagttcgta atgcaaggct tcgatttcga aggctttccc accctgatgg atgccttgat
     5761 cttgcatcgc ctgattgaaa gtgtctacaa gtcgaatatg atgggcagta ccctgaatgt
     5821 ttccaacatc tcccactact cgctttaaac gcgtaggatc tttgtgaagg aaccttactt
     5881 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     5941 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     6001 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     6061 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     6121 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     6181 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     6241 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt tgatgtatag
     6301 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     6361 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     6421 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6481 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6541 atcatgtctg gatccctagg ataacttcgt ataatgtatg ctatacgaag ttatggtacc
     6601 aaaaggtagg ttcaaccact gatgcctagg cacaccgaaa cgactaaccc taattcttat
     6661 cctttacttc aggaacttaa aaaaaaaaat caaaatgggc aacaaatgct gcagcaagcg
     6721 acaggatcag gaactggcac tggcctatcc cactgggggc tacaagaaat ccgactacac
     6781 ctttggccag acgcacatca acagcagcgg cggcggcaac atgggcggcg ttcttggcca
     6841 gaagcataac aacggtggct cgctggactc gcgctacacg cccgatccca atcatcgggg
     6901 tccgttgaaa atcggcggaa agggcggcgt tgacatcatc agaccacgcg gatccatggt
     6961 gtcgaaaggc gaggaggtta tcaaggagtt tatgcgattt aaggtccgaa tggagggcag
     7021 tatgaatggt catgaatttg agattgaggg tgaaggagag ggccgaccgt acgaaggtac
     7081 gcagacggcg aagttgaagg tcacgaaagg aggacctctg ccattcgcct gggatatcct
     7141 gtcccctcag tttatgtacg gaagcaaggc atacgtgaag caccccgccg atattccaga
     7201 ttataagaaa ctctccttcc ccgagggatt caagtgggag cgcgttatga actttgagga
     7261 tggaggactt gtcacggtca ctcaggacag cagtctgcag gacggaaccc tgatctacaa
     7321 ggttaagatg cgaggtacga acttcccacc ggatggtccg gtgatgcaga aaaagacgat
     7381 gggatgggag gcgagcaccg agcgactgta cccgcgtgat ggagtcctca agggcgaaat
     7441 tcatcaggcc ctgaaattga aagatggtgg ccactatttg gtggagttca agactatcta
     7501 catggcaaag aagcccgtac agctgccggg ctattattac gttgacacga agctcgatat
     7561 cacgagtcat aacgaggact acacaatcgt tgagcaatac gagcgcagcg agggtcgcca
     7621 ccaccttttc ctgggacatg gcactggctc gaccggctcg ggcagcagcg gtacagcaag
     7681 cagtgaggac aacaacatgg ccgtgattaa ggaatttatg aggttcaagg tacgtatgga
     7741 gggttccatg aacggccatg agttcgagat tgagggagag ggtgaaggcc gaccgtacga
     7801 aggcacgcag actgcgaaac tgaaggtgac aaagggtggc ccgctgccat ttgcctggga
     7861 tattctgagc cctcagttca tgtacggtag caaggcatac gtcaaacacc ctgcagatat
     7921 ccccgattac aagaagttgt cctttcccga gggttttaag tgggagcggg tgatgaactt
     7981 cgaagacgga ggtcttgtca cggtgacaca ggatagttcc ctgcaggacg gcactctgat
     8041 ctacaaagtc aagatgcgag gcaccaattt tccaccggat ggaccagtga tgcaaaagaa
     8101 gaccatgggt tgggaagcta gtacggaacg cctgtatccg agggatggtg tgctcaaggg
     8161 cgagatccac caggcattga aacttaagga cggtggacat tacttggtcg agttcaaaac
     8221 tatttacatg gctaagaaac ccgtccagct gcccggctac tattatgtcg ataccaagct
     8281 ggacatcacc tcccacaatg aagactacac catcgtggag cagtacgaac gcagtgaggg
     8341 ccgccaccac ttgttcctgt acggtatgga cgagctttac aagtaaaggc ctatgaatcg
     8401 tttttaaaat aacaaatcaa ttgttttata atattcgtac gattctttga ttatgtaata
     8461 aaatgtgatc attaggaaga ttacgaaaaa tataaaaaat atgagttctg tgtgtataac
     8521 aaatgctgta aacgccacaa ttgtgtttgt tgcaaataaa cccatgatta tttgattaaa
     8581 attgttgttt tctttgttca tagacaatag tgtgttttgc ctaaacgtgt actgcataaa
     8641 ctccatgcga gtgtatagcg agctagtggc taacgcttgc cccaccaaag tagattcgtc
     8701 aaaatcctca atttcatcac cctcctccaa gtttaacatt tggccgtcgg aattaacttc
     8761 taaagatgcc acataatcta ataaatgaaa tagagattca aacgtggcgt catcgtccgt
     8821 ttcgaccatt tccgaaaaga actcgggcat aaactctatg atttctctgg acgtggtgtt
     8881 gtcgaaactc tcaaagtacg cagtcaggaa cgtgcgcgac atgtcgtcgg gaaactcgcg
     8941 cggaaacatg ttgttgtaac cgaacgggtc ccatagcgcc aaaaccaaat ctgccagcgt
     9001 caatagaatg agcacgatgc cgacaatgga gctggcttgg atagcgattc gagttaacct
     9061 agttctagag cggccgctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc
     9121 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa
     9181 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg
     9241 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc
     9301 ggaggcgcgc cctagttcca gtgaaatcca agcattttct aaattaaatg tattcttatt
     9361 attatagttg ttatttttga tatatataaa caacactatt atgcccacca tttttttgag
     9421 atgcatctac acaaggaaca aacactggat gtcactttca gttcaaattg taacgctaat
     9481 cactccgaac aggtcacaaa aaattacctt aaaaagtcat aatattaaat tagaataaat
     9541 atagctgtga gggaaatata tacaaatata ttggagcaaa taaattgtac atacaaatat
     9601 ttattactaa tttctattga gacgaaatga accactcgga accatttgag cgaaccgaat
     9661 cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga
     9721 gagcgggtgg gagacagcga aagagcaact acgaaacgtg gtgtggtgga ggtgaattat
     9781 gaagagggcg cgcgatttga aaagtatgta tataaaaaat atatcccggt gttttatgta
     9841 gcgataaacg agtttttgat gtaaggtatg caggtgtgta agtcttttgg ttagaagaca
     9901 aatccaaagt ctacttgtgg ggatgttcga aggggaaata cttgtattct ataggtcata
     9961 tcttgttttt attggcacaa atataattac attagctttt tgagggggca ataaacagta
    10021 aacacgatgg taataatggt aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc
    10081 tactccttgc gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg
    10141 atgagaatgg ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc
    10201 tccaggatga ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg
    10261 cccggctcca cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat
    10321 gagaggtacg acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaagccg
    10381 ccaaagagca ggaatggtat gataaccggc ggacccacag acagcgccat cgaggtcgag
    10441 gagctggcgc aggatattag atatccgaag gacgttgaca cattggccac cagagtgacc
    10501 agcgccaggc agttgaagaa gtgcagcact ccggcccgca gtccgatcat cggataggca
    10561 atcgccgtga agaccagtgg cactgtgaga aaaagcggca attcggcaat cgttttgccc
    10621 agaaagtatg tgtcacagcg ataaagtcga cttcgggcct ccctcataaa aactggcagc
    10681 tctgaggtga acacctaaat cgaatcgatt cattagaaag ttagtaaatt attgaaatgc
    10741 aaatgtattc taaacatgac ttacatttat cgtggcaaag acgttttgaa aggtcatgtt
    10801 ggtcaggaag aggaagatgg ctccgttgat attcatcaca cccacttgcg tgagttgttg
    10861 gcccaaaaag atgaggccaa tcaagatggc aaccatctgc aaattaaaat gttactcgca
    10921 tctcattaat attcgcgagt taaatgaaat ttatttatct tctgcaaaac tataaactat
    10981 acatctcatt gaaaaaaact aagaagggtg tggaatcagg caattctatc taaaatctag
    11041 cgaatttgtt tccaagaatt gtaagcgtta tatcatttgt ttccactgga accactcacc
    11101 gttgtctgaa taagtcgcac ttttacgagg agtggttcct tgagcaccga cagccaggat
    11161 cgccacagga ccgcccggaa ctgcatgaac caggtggcct tgtaggtgta cccattctcc
    11221 ggctgctcca gtggcttctc cagatttttg gtggccaaca actgctccat atcccgggct
    11281 actttgctaa tggcaaaatt gtcgcatatc ttggcgatcc gatcacggga ctcgatctcc
    11341 cgtccgggca caacggccaa cacctgtacg taaaagtccg ccggattgta gttggtagga
    11401 cactgggcac ccacgctgga taggagttga gatgttatgt aatactagat acccttaata
    11461 aacacatcga actcactagg aaaagaagtc gacggcttcg ctgggagtgc ccaagaaagc
    11521 taccctgccc tcggccatca gaaggatctt gtcaaagagc tcaaacagct cggaagacgg
    11581 ctgatgaatg gtcaggatga cggtcttgcc cttctgcgac agcttcttca gcacctggac
    11641 gacgctgtgg gcggtaaagg agtccagtcc ggaggtgggc tcatcgcaga tcagaagcgg
    11701 cggatcggtt agagcctcgg aggcgaatgc cagacgcttc ctttctccgc cggacagacc
    11761 tttcaccctg ccgggcacac cgatgatcgt gtgctgacat ttgctgagcg aaagctcctg
    11821 gatcacctga tccacgcggg ccactcgctg ccgataggtc agatgtcgtg gcatccgcac
    11881 catggcttgg aaaatcaggt gttccctggc cgttagggag ccgataaaga ggtcatcctg
    11941 ctggacatag gcgcacctgg cctgcatctc cttggcgtcc acaggttggc cattgagcag
    12001 tcgcatcccg gatggcgata cttggatgcc ctgcggcgat cgaaaggcaa gggcattcag
    12061 cagggtcgtc tttccggcac cggaactgcc catcacggcc aaaagttcgc ccggataggc
    12121 cacgccgcaa actgagtttc aaattggtaa ttggaccctt tattaagatt tcacacagat
    12181 cagccgactg cgaatagaaa ctcaccgttc ttgagcaaat gtttcctggg cgccggtatg
    12241 tgtcgctcgt tgcagaatag tccgcgtgtc cggttgacca gctgccgcca tccggagccc
    12301 ggctgattga ccgccccaaa gatgtccata ttgtgccagg cataggtgag gttctcggct
    12361 agttggccgc tccctgaacc ggagtcctcc ggcggactgg gtggccggag cgtgccgtag
    12421 tttttggcct gcccgaagcc ctggttaatg cagctctgcg aagccgctcc gctgtcaccc
    12481 tgcaatgata ggggatctca aatatcaact actagcgtta tgctcatcta accccgaaca
    12541 aaaagtaccc cgaagtatcc tacgaagtag gtttatactt ttatttattt tttgtgcatc
    12601 taggatcagc ttaaaatatc tggttgttat attttttgta aaaaagaata tagtcgaaaa
    12661 tgaatgcctt tagatgtctt gatcatgata tgatctcaaa aattgtctta tatagcgaga
    12721 acagctacca gaataatctg tttcgtgtca ctatttgttt gtgcaattgc ggtttgggat
    12781 ttttgtgggt cgcagttctc acgccgcata caatttgatg ttgcaatcgc agttcctata
    12841 gatcaagtga acttaagatg tatgcacatg tactactcac attgttcaga tgctcggcag
    12901 atgggtgttt gctgcctccg cgaattaata gctcctgatc ctcttggccc attgccggga
    12961 tttttcacac tttcccctgc ttacccaccc aaaaccaatc accaccccaa tcactcaaaa
    13021 aacaaacaaa aataagaagc gagaggagtt ttggcacagc actttgtgtt taattgatgg
    13081 cgtaaaccgc ttggagcttc gtcacgaaac cgctgacaaa atgcaactga aggcggacat
    13141 tgacgctacg taacgctaca aacggtggcg aaagagatag cggacgcagc ggcgaaagag
    13201 acggcgatat ttctgtggac agagaaggag gcaaacagcg ctgactttga gtggaatgtc
    13261 attttgagtg agaggtaatc gaaagaacct ggtacatcaa atacccttgg atcgaagtaa
    13321 atttaaaact gatcagataa gttcaatgat atccagtgca gtaaaaaaaa aaaatgtttt
    13381 ttttatctac tttccgcaaa aatgggtttt attaacttac atacatacta ggcgcgccca
    13441 taagcttgca tgcctgcagg tcggagtact gtcctccgag cggagtactg tcctccgagc
    13501 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
    13561 gactctagcc ctagggcatg cctgcaggtc ggagtactgt cctccgagcg gagtactgtc
    13621 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
    13681 cgagcggaga ctctagcgct agcgacgtcg agcgccggag tataaataga ggcgcttcgt
    13741 ctacggagcg acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa
    13801 gcaaataaac aagcgcagct gaacaagcta aacaatctgc agtaaagtgc aagttaaagt
    13861 gaatcaatta aaagtaacca gcaaccaagt aaatcaactg caactactga aatctgccaa
    13921 gaagtaatta ttgaatacaa gaagagaact ctgaatagat ctgcggccgc ggctcgagca
    13981 aaatggcctc accgttgacc cgctttctgt cgctgaacct gctgctgctg ggtgagtcga
    14041 ttatcctggg gagtggagaa gctaagccac aggcacccga actccgaatc tttccaaaga
    14101 aaatggacgc cgaacttggt cagaaggtgg acctggtatg tgaagtgttg gggtccgttt
    14161 cgcaaggatg ctcttggctc ttccagaact ccagctccaa actcccccag cccaccttcg
    14221 ttgtctatat ggcttcatcc cacaacaaga taacgtggga cgagaagctg aattcgtcga
    14281 aactgttttc tgccatgagg gacacgaata ataagtacgt tctcaccctg aacaagttca
    14341 gcaaggaaaa cgaaggctac tatttctgct cagtcatcag caactcggtg atgtacttca
    14401 gttctgtcgt gccagtcctt cagaaagtga actctactac taccaagcca gtgctgcgaa
    14461 ctccctcacc tgtgcaccct accgggacat ctcagcccca gagaccagaa gattgtcggc
    14521 cccgtggctc agtgaagggg accggattgg acttcgcctg tgatatttac atctgggcac
    14581 ccttggccgg aatctgcgtg gcccttctgc tgtccttgat catcactctc atctgctacc
    14641 acagccgcgg atccatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
    14701 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
    14761 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
    14821 catggccaac acttgtcact actttaactt atggtgttca atgcttttca agatacccag
    14881 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtccaggaaa
    14941 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
    15001 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
    15061 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
    15121 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
    15181 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
    15241 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
    15301 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
    15361 tatacaaata atctagagga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
    15421 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
    15481 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
    15541 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
    15601 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
    15661 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
    15721 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
    15781 actgctatac aagaaaatta tggaaaaata tttgatgtat agtgccttga ctagagatca
    15841 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
    15901 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
    15961 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    16021 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaccg
    16081 gttatgttcg gcttgtcgac atgcccgccg tgaccgtcga gaacccgctg acgctgcccc
    16141 gcgtatccgc acccgccgac gccgtcgcac gtcccgtgct caccgtgacc accgcgccca
    16201 gcggtttcga gggcgagggc ttcccggtgc gccgcgcgtt cgccgggatc aactaccgcc
    16261 acctcgaccc gttcatcatg atggaccaga tgggtgaggt ggagtacgcg cccggggagc
    16321 ccaagggcac gccctggcac ccgcaccgcg gcttcgagac cgtgacctac atcgtcgacg
    16381 gtaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    16441 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    16501 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    16561 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    16621 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    16681 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    16741 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    16801 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    16861 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    16921 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    16981 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    17041 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    17101 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    17161 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    17221 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    17281 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    17341 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    17401 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    17461 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    17521 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    17581 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    17641 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    17701 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    17761 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    17821 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    17881 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    17941 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    18001 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    18061 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    18121 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    18181 gaaaagtgcc acctgagtc
//
