LOCUS       sequence-314818-       10950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Z. mobilis CRISPRi, promoter C, empty guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10950)
  AUTHORS   Banta AB, Enright AL, Siletti C, Peters JM
  TITLE     A High-Efficacy CRISPR Interference System for Gene Function 
            Discovery in Zymomonas mobilis.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 10;86(23). pii: AEM.01621-20. doi: 
            10.1128/AEM.01621-20. Print 2020 Nov 10.
  PUBMED    32978126
REFERENCE   2  (bases 1 to 10950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2020-11-10"; volume: "86(23). pii: 
            AEM.01621-20. doi"; pages: " 10.1128/AEM.01621-20. Print 2020 Nov 
            10"
COMMENT     Sequence Label: pJMP2480
FEATURES             Location/Qualifiers
     source          1..10950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..78
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             79..1161
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(98..117)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    1437..1456
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    complement(1877..1924)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(2001..2006,2007..2023,2024..2029)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     complement(2178..2197)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    complement(2861..2908)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(2959..3045)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(3148..3242)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(3270..3286)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3271..3287)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3701..3720)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3820..3842
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3880..3898)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3966..4070
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4071..4139,4140..4931)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4289..4308)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5232..5620
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            5889..5997
                     /note="incP origin of transfer"
     mobile_element  6156..6321
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(6476..6565)
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             complement(join(6575..8158,8159..8161,8162..10648,
                     10649..10651,10652..10678))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    10701..10717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    10734..10750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt
       61 caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
      121 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
      181 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
      241 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
      301 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
      361 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
      421 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
      481 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
      541 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
      601 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
      661 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
      721 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
      781 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
      841 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
      901 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
      961 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
     1021 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
     1081 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     1141 cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc
     1201 accccacccg ggtccttgag ccccattatc acctccaaag ccttctcgtc tggtcagttt
     1261 cacctgtttt acgtaaaaac ccgcttcggc gggtttttac ttttggggaa acacagaaaa
     1321 aagcccgcac ctgacagtgc gggctttttt tttcgaccaa aggtgcgact actcttgcct
     1381 actacctatc gactgagctg aaagaattcg gaaaattttt tttcaaaagt acttgaaatt
     1441 gtgagcgctc acaattataa ttctagtaga gaccaacttt ggtctccacc atagcggtcg
     1501 gtctctgttt aagagctatg ctggaaacag catagcaagt ttaaataagg ctagtccgtt
     1561 atcaacttga aaaagtggca ccgagtcggt gctttttttt tgaattcatg tggctgaccg
     1621 ttctgttgtc tctcgctctt ccgagtagac gaacaataag gcctccctaa cggggggcct
     1681 tttttattga taacaaaagt cagtgcttcc gctatttcca aaataccggg ctaatacggt
     1741 ttaaacgacc tcctggattt gctcagacag ccttttcgtc attcgtttca gccaaaaaac
     1801 ttaagaccgc cggtcttgtc cactaccttg cagtaatgcg gtggacagga tcggcggttt
     1861 tcttttctct tctcaagaag ttcctatact ttctagagaa taggaacttc ggaataggaa
     1921 cttcctcctg aacggccata agaacgaagg ctgtctgttg aactctcgag ccgtggaaac
     1981 ggatgaaggc acgaacccag ttgacataag cctgttcggt tcgtaaactg taatgcaagt
     2041 agcgtatgcg ctcatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
     2101 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     2161 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     2221 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     2281 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggagttc cgtatggcaa
     2341 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     2401 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     2461 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     2521 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     2581 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     2641 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     2701 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtttttatgc atgcgcccaa
     2761 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     2821 ttcccgactg gaactcgagc atcagcttca aaagcgctct gaagttccta tactttctag
     2881 agaataggaa cttcggaata ggtacttcaa gatccccaat tcgagctcat cgtccgggcc
     2941 gcaagctcct agcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     3001 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tcaagctaga
     3061 gagtcattac cccaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc
     3121 cctgccactc atcgcagtct agcttggatt ctcaccaata aaaaacgccc ggcggcaacc
     3181 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     3241 tccaagctca gctaattaag ctagcttatc gataccgtcg acctcgaacc ccacgcccct
     3301 ctttaatacg acgggcaatt tgcacttcag aaaatgaaga gtttgcttta gccataacaa
     3361 aagtccagta tgctttttca cagcataact ggactgattt cagtttacaa ctattctgtc
     3421 tagtttaaga ctttattgtc atagtttaga tctattttgt tcagtttaag actttattgt
     3481 ccgcccacac ctgcttacgc agggcatcca tttattactc aaccgtaacc gattttgcca
     3541 ggttacgcgg ctggtcctct agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     3601 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     3661 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     3721 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3781 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     3841 ggttttcacc gtcgtcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     3901 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     3961 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4021 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4081 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4141 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4201 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4261 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4321 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4381 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4441 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4501 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4561 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4621 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4681 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4741 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtcc cgcggtatca
     4801 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4861 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4921 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4981 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5041 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggcagtt
     5101 attggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5161 tagattgatt taaaacttca tttttaattt ttgcggccgc aagatccggc cacgatgcgt
     5221 ccggcgtaga ggatctgaag atcagcagtt caacctgttg atagtacgta ctaagctctc
     5281 atgtttcacg tactaagctc tcatgtttaa cgtactaagc tctcatgttt aacgaactaa
     5341 accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg
     5401 tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct
     5461 aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc ttttaaggtt
     5521 taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta gagcctctca
     5581 aagcaatttt gagtgacaca ggaacactta acggctgaca tgggaattag ccatggcatc
     5641 acagtatcgt gatgacagag gcagggagtg cggccttttt acggttcctg gccttttgct
     5701 ggccttttgc tcacatgttc tttcctgcgt tatcagggga ttccttaagg tatactttcc
     5761 gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc
     5821 gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg
     5881 cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact
     5941 gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgata
     6001 agctctgata ccgctcgccg cagccgaacg accgagcgca gcgggtcagt gagcgaggaa
     6061 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6121 agcgcccaaa cataacagga agaaaaatgc cccgctgtgg gcggacaaaa tagttgggaa
     6181 ctgggagggg tggaaatgga gtttttaagg attatttagg gaagagtgac aaaatagatg
     6241 ggaactgggt gtagcgtcgt aagctaatac gaaaattaaa aatgacaaaa tagtttggaa
     6301 ctagatttca cttatctggt tcttgaggcg gttaaaagag ccgtactctt ctccgatgtc
     6361 gactaggcca tgatgctcat tctgtgggac caaaacgaaa aaacaccctt tcgggtgtct
     6421 tttctggaat ttggtaccga ggctgcaagt cccattaagg aggcgcgcct tattacagat
     6481 cttcctcaga gatgagcttc tgttccagat cttcttctga aatcaacttt tgttccagat
     6541 cttcttcaga gatgagtttc tgctccgcgg ccgcgtcacc tcctagctga ctcaaatcaa
     6601 tgcgtgtttc ataaagacca gtgatggatt gatggataag agtggcatct aaaacttctt
     6661 ttgtagacgt atatcgttta cgatcaattg ttgtatcaaa atatttaaaa gcagcgggag
     6721 ctccaagatt cgtcaacgta aataaatgaa taatattttc tgcttgttca cgtattggtt
     6781 tgtctctatg tttgttatat gcactaagaa ctttatctaa attggcatct gctaaaataa
     6841 cacgcttaga aaattcactg atttgctcaa taatctcatc taaataatgc ttatgctgct
     6901 ccacaaacaa ttgtttttgt tcgttatctt ctggactacc cttcaacttt tcataatgac
     6961 tagctaaata taaaaaattc acatatttgc ttggcagagc cagctcattt cctttttgta
     7021 attctccggc actagccagc atccgtttac gaccgttttc taactcaaaa agactatatt
     7081 taggtagttt aatgattaag tcttttttaa cttccttata tcctttagct tctaaaaagt
     7141 caatcggatt tttttcaaag gaacttcttt ccataattgt gatccctagt aactctttaa
     7201 cggattttaa cttcttcgat ttcccttttt ccaccttagc aaccactagg actgaataag
     7261 ctaccgttgg actatcaaaa ccaccatatt tttttggatc ccagtctttt ttacgagcaa
     7321 taagcttgtc cgaatttctt tttggtaaaa ttgactcctt ggagaatccg cctgtctgta
     7381 cttctgtttt cttgacaata ttgacttggg gcatggacaa tactttgcgc actgtggcaa
     7441 aatctcgccc tttatcccag acaatttctc cagtttcccc attagtttcg attagagggc
     7501 gtttgcgaat ctctccattt gcaagtgtaa tttctgtttt gaagaagttc atgatattag
     7561 agtaaaagaa atattttgcg gttgctttgc ctatttcttg ctcagactta gcaatcattt
     7621 tacgaacatc ataaacttta taatcaccat agacaaactc cgattcaagt tttggatatt
     7681 tcttaatcaa agcagttcca acgacggcat ttagatacgc atcatgggca tgatggtaat
     7741 tgttaatctc acgtacttta tagaattgga aatcttttcg gaagtcagaa actaatttag
     7801 attttaaggt aatcacttta acctctcgaa taagtttatc attttcatcg tatttagtat
     7861 tcatgcgact atccaaaatt tgtgccacat gcttagtgat ttggcgagtt tcaaccaatt
     7921 ggcgtttgat aaaaccagct ttatcaagtt cactcaaacc tccacgttca gctttcgtta
     7981 aattatcaaa cttacgttga gtgattaact tggcgtttag aagttgtctc caatagtttt
     8041 tcatcttttt gactacttct tcacttggaa cgttatccga tttaccacga tttttatcag
     8101 aacgcgttaa gaccttattg tctattgaat cgtctttaag gaaactttgt ggaacaatgg
     8161 catcgacatc ataatcactt aaacgattaa tatctaattc ttggtccaca tacatgtctc
     8221 ttccattttg gagataatag agatagagct tttcattttg caattgagta ttttcaacag
     8281 gatgctcttt aagaatctga cttcctaatt ctttgatacc ttcttcgatt cgtttcatac
     8341 gctctcgcga atttttctgg cccttttgag ttgtctgatt ttcacgtgcc atttcaataa
     8401 cgatattttc tggcttatgc cgccccatta ctttgaccaa ttcatcaaca acttttacag
     8461 tctgtaaaat acctttttta atagcagggc taccagctaa atttgcaata tgttcatgta
     8521 aactatcgcc ttgtccagac acttgtgctt tttgaatgtc ttctttaaat gtcaaactat
     8581 catcatggat cagctgcata aaattgcgat tggcaaaacc atctgatttc aaaaaatcta
     8641 atattgtttt gccagattgc ttatccctaa taccattaat caattttcga gacaaacgtc
     8701 cccaaccagt ataacggcga cgtttaagct gtttcatcac cttatcatca aagaggtgag
     8761 catatgtttt aagtctttcc tcaatcatct ccctatcttc aaataaggtc aatgttaaaa
     8821 caatatcctc taagatatct tcattttctt cattatccaa aaaatcttta tctttaataa
     8881 tttttagcaa atcatggtag gtacctaatg aagcattaaa tctatcttca actcctgaaa
     8941 tttcaacact atcaaaacat tctatttttt tgaaataatc ttcttttaat tgcttaacgg
     9001 ttacttttcg atttgttttg aagagtaaat caacaatggc tttcttctgt tcacctgaaa
     9061 gaaatgctgg ttttcgcatt ccttcagtaa catatttgac ctttgtcaat tcgttataaa
     9121 ccgtaaaata ctcataaagc aaactatgtt ttggtagtac tttttcattt ggaagatttt
     9181 tatcaaagtt tgtcatgcgt tcaataaatg attgagctga agcaccttta tcgacaactt
     9241 cttcaaaatt ccatggggta attgtttctt cagacttccg agtcatccat gcaaaacgac
     9301 tattgccacg cgccaatgga ccaacataat aagggattcg aaaagtcaag attttttcaa
     9361 tcttctcacg attgtctttt aaaaatggat aaaagtcttc ttgtcttctc aaaatagcat
     9421 gcagctcacc caagtgaatt tgatggggaa tagagccgtt gtcaaaggtc cgttgcttgc
     9481 gcagcaaatc ttcacgattt agtttcacca ataattcctc agtaccatcc attttttcta
     9541 aaattggttt gataaattta taaaattctt cttggctagc tcccccatca atataacctg
     9601 catatccgtt ttttgattga tcaaaaaaga tttctttata cttttctgga agttgttgtc
     9661 gaactaaagc ttttaaaaga gtcaagtctt gatgatgttc atcgtagcgt ttaatcattg
     9721 aagctgatag gggagcctta gttatttcag tatttactct taggatatct gaaagtaaaa
     9781 tagcatctga taaattctta gctgccaaaa acaaatcagc atattgatct ccaatttgcg
     9841 ccaataaatt atctaaatca tcatcgtaag tatcttttga aagctgtaat ttagcatctt
     9901 ctgccaaatc aaaatttgat ttaaaattag gggtcaaacc caatgacaaa gcaatgagat
     9961 tcccaaataa gccatttttc ttctcaccgg ggagctgagc aatgagattt tctaatcgtc
    10021 ttgatttact caatcgtgca gaaagaatcg ctttagcatc tactccactt gcgttaatag
    10081 ggttttcttc aaataattga ttgtaggttt gtaccaactg gataaatagt ttgtccacat
    10141 cactattatc aggatttaaa tctccctcaa tcaaaaaatg accacgaaac ttaatcatat
    10201 gcgctaaggc caaatagatt aagcgcaaat ccgctttatc agtagaatct accaattttt
    10261 ttcgcagatg atagatagtt ggatatttct catgataagc aacttcatct actatatttc
    10321 caaaaatagg atgacgttca tgcttcttgt cttcttccac caaaaaagac tcttcaagtc
    10381 gatgaaagaa actatcatct actttcgcca tctcatttga aaaaatctcc tgtagataac
    10441 aaatacgatt cttccgacgt gtataccttc tacgagctgt ccgtttgaga cgagtcgctt
    10501 ccgctgtctc tccactgtca aataaaagag cccctataag attttttttg atactgtggc
    10561 ggtctgtatt tcccagaacc ttgaactttt tagacggaac cttatattca tcagtgatca
    10621 ccgcccatcc gacgctattt gtgccgatag ctaagcctat tgagtatttc ttatccatac
    10681 tagtcctcct tctcgctcaa ttgttatccg ctcacaattg aatctaagta tcattgttat
    10741 ccgctcacaa gtcaacactc tttttgataa attttactag tctgcagacg taaaaaaagc
    10801 ggcgtggtta gccgcttttt taattgccgg agcgcaataa aaaagccccc ggaaggtgat
    10861 cttccggggg ctttctcatg cgttgcttgt attgaaaatg ggggccatgc ctacctatct
    10921 gcctcccggg aagcggcatg catttacgtt
//
