LOCUS       sequence-31482-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GNPNAT1
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             670..687
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(740..758)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      754..801
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaatattttg tttactttaa gaaggagata taccatgcat catcatcatc atcacagcag
       61 cggcagagaa aacttgtatt tccagggcag gaaacctgat gaaactccta tgtttgaccc
      121 aagtctactc aaagaagtgg actggagtca gaatacagct acattttctc cagccatttc
      181 cccaacacat cctggagaag gcttggtttt gaggcctctt tgtactgctg acttaaatag
      241 aggttttttt aaggtattgg gtcagctaac agagactgga gttgtcagcc ctgaacaatt
      301 tatgaaatct tttgagcata tgaagaaatc tggggattat tatgttacag ttgtagaaga
      361 tgtgactcta ggacagattg ttgctacggc aactctgatt atagaacata aattcatcca
      421 ttcctgtgct aagagaggaa gagtagaaga tgttgttgtt agtgatgaat gcagaggaaa
      481 gcagcttggc aaattgttat tatcaaccct tactttgcta agcaagaaac tgaactgtta
      541 caagattacc cttgaatgtc taccacaaaa tgttggtttc tataaaaagt ttggatatac
      601 tgtatctgaa gaaaactaca tgtgtcggag gtttctaaag tgatgacgaa gcttgcggcc
      661 gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa
      721 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      781 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg
      841 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      901 cacttgccag cgccctagcg cccgctcctt tcgc
//
