LOCUS       sequence-314822-       13045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a concatemeric construct of a alpha-4:beta-2:alpha-5 
            (2:2:1) nAChR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13045)
  AUTHORS   Prevost MS, Bouchenaki H, Barilone N, Gielen M, Corringer PJ
  TITLE     Concatemers to re-investigate the role of alpha5 in alpha4beta2 
            nicotinic receptors.
  JOURNAL   Cell Mol Life Sci. 2020 May 29. pii: 10.1007/s00018-020-03558-z. 
            doi: 10.1007/s00018-020-03558-z.
  PUBMED    32472188
REFERENCE   2  (bases 1 to 13045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2020 May 29. pii: 10.1007/s00018-020-03558-z. doi: 
            10.1007/s00018-020-03558-z."
COMMENT     Sequence Label: pRK5-alpha-5first
FEATURES             Location/Qualifiers
     source          1..13045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      955..964
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      959..968
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      9050..9059
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    9063..9197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9100..9119)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9154..9173
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        9266..9623
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9266..9457
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9289..9309)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9474..9609
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9643..9660)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9643..9659)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9652..9674)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9959..9978)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(10043..10234)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     10464..10485
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10639..10658)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10818..10836)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10904..11008
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11009..11077,11078..11869)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11227..11246)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12040..12628
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12529..12548
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12782..12799
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12916..12937
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12952..12957,12958..12975,12976..12982)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12990..13006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12995..13017
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13014..13030
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13014..13030
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tgcagaagct cagaataaac gctcaacttt ggcagatcat cgatgccacc
      961 atggcggcgc gggggtcagg gccccgcgcg ctccgcctgc tgctcttggt ccagctggtc
     1021 gcggggcgct gcggtctagc gggcgcggcg ggcggcgcgc agagaggatt atctgaacct
     1081 tcttctattg caaaacatga agatagtttg cttaaggatt tatttcaaga ctacgaaaga
     1141 tgggttcgtc ctgtggaaca cctgaatgac aaaataaaaa taaaatttgg acttgcaata
     1201 tctcaattgg tggatgtgga tgagaaaaat cagttaatga caacaaacgt ctggttgaaa
     1261 caggaatgga tagatgtaaa attaagatgg aaccctgatg actatggtgg aataaaagtt
     1321 atacgtgttc cttcagactc tgtctggaca ccagacatcg ttttgtttga taatgcagat
     1381 ggacgttttg aagggaccag tacgaaaaca gtcatcaggt acaatggcac tgtcacctgg
     1441 actccaccgg caaactacaa aagttcctgt accatagatg tcacgttttt cccatttgac
     1501 cttcagaact gttccatgaa atttggttct tggacttatg atggatcaca ggttgatata
     1561 attctagagg accaagatgt agacaagaga gatttttttg ataatggaga atgggagatt
     1621 gtgagtgcaa cagggagcaa aggaaacaga accgacagct gttgctggta tccgtatgtc
     1681 acttactcat ttgtaatcaa gcgcctgcct ctcttttata ccttgttcct tataataccc
     1741 tgtattgggc tctcattttt aactgtactt gtcttctatc ttccttcaaa tgaaggtgaa
     1801 aagatttgtc tctgcacttc agtacttgtg tctttgactg tcttccttct ggttattgaa
     1861 gagatcatac catcatcttc aaaagtcata cctctaattg gagagtatct ggtatttacc
     1921 atgatttttg tgacactgtc aattatggta accgtcttcg ctatcaacat tcatcatcgt
     1981 tcttcctcaa cacataatgc catggcgcct ttggtccgca agatatttct tcacacgctt
     2041 cccaaactgc tttgcatgag aagtcatgta gacaggtact tcactcagaa agaggaaact
     2101 gagagtggta gtggaccaaa atcttctaga aacacattgg aagctgcgct cgattctatt
     2161 cgctacatta caagacacat catgaaggaa aatgatgtcc gtgaggttgt tgaagattgg
     2221 aaattcatag cccaggttct tgatcggatg tttctgtgga cttttctttt cgtttcaatt
     2281 gttggatctc ttgggctttt tgttcctgtt atttataaat gggcaaatat attaatacca
     2341 gttcatattg gaaatgcaaa taagcaacag caacagcagc aacagcagca acaacagcaa
     2401 cagcagcaaa ccggtacgga tacagaggag cggctggtgg agcatctcct ggatccttcc
     2461 cgctacaaca agctcatccg cccagccacc aatggctctg agctggtgac agtacagctt
     2521 atggtgtcac tggcccagct catcagtgtg catgagcggg agcagatcat gaccaccaat
     2581 gtctggctga cccaggagtg ggaagattat cgcctcacct ggaagcctga agagtttgac
     2641 aacatgaaga aagttcggct cccttccaaa cacatctggc tcccagatgt ggtcctgtac
     2701 aacaatgctg acggcatgta cgaggtgtcc ttctattcca atgccgtggt ctcctatgat
     2761 ggcagcatct tctggctgcc gcctgccatc tacaagagcg catgcaagat tgaagtaaag
     2821 cacttcccat ttgaccagca gaactgcacc atgaagttcc gttcgtggac ctacgaccgc
     2881 acagagatcg acttggtgct gaagagtgag gtggccagcc tggacgactt cacacctagt
     2941 ggtgagtggg acatcgtggc gctgccgggc cggcgcaacg agaaccccga cgactctacg
     3001 tacgtggaca tcacgtatga cttcatcatt cgccgcaagc cgctcttcta caccatcaac
     3061 ctcatcatcc cctgtgtgct catcacctcg ctggccatcc ttgtcttcta cctgccatcc
     3121 gactgtggcg agaagatgac gttgtgcatc tcagtgctgc tggcgctcac ggtcttcctg
     3181 ctgctcatct ccaagatcgt gcctcccacc tccctcgacg tgccgctcgt cggcaagtac
     3241 ctcatgttca ccatggtgct tgtcaccttc tccatcgtca ccagcgtgtg cgtgctcaac
     3301 gtgcaccacc gctcgcccac cacgcacacc atggcgccct gggtgaaggt cgtcttcctg
     3361 gagaagctgc ccgcgctgct cttcatgcag cagccacgcc atcattgcgc ccgtcagcgc
     3421 ctgcgcctgc ggcgacgcca gcgtgagcgc gagggcgctg gagccctctt cttccgcgaa
     3481 gccccagggg ccgactcctg cacgtgcttc gtcaaccgcg cgtcggtgca ggggttggcc
     3541 ggggccttcg gggctgagcc tgcaccagtg gcgggccccg ggcgctcagg ggagccgtgt
     3601 ggctgtggcc tccgggaggc ggtggacggc gtgcgcttca tcgcagacca catgcggagc
     3661 gaggacgatg accagagcgt gagtgaggac tggaagtacg tcgccatggt gatcgaccgc
     3721 ctcttcctct ggatctttgt ctttgtctgt gtctttggca ccatcggcat gttcctgcag
     3781 cctctcttcc agaactacac caccaccacc ttcctccact cagaccactc agcccccagc
     3841 tccaagcagc aacagcagca acaacagcaa cagcagcaac agcagcaaca agtcgaccat
     3901 gtggagaccc gggcccacgc cgaggagcgg ctcctgaaga aactcttctc cggttacaac
     3961 aagtggtccc gacccgtggc caacatctcg gacgtggtcc tcgtccgctt cggcctgtcc
     4021 atcgctcagc tcattgacgt ggatgagaag aaccagatga tgaccacgaa cgtatgggtg
     4081 aagcaggagt ggcacgacta caagctgcgc tgggacccag ctgactatga gaatgtcacc
     4141 tccatccgca tcccctccga gctcatctgg cggccggaca tcgtcctcta caacaatgct
     4201 gacggggact tcgcggtcac ccacctgacc aaggcccacc tgttccatga cgggcgggtg
     4261 cagtggactc ccccggccat ttacaagagc tcctgcagca tcgacgtcac cttcttcccc
     4321 ttcgaccagc agaactgcac catgaaattc ggctcctgga cctacgacaa ggccaagatc
     4381 gacctggtga acatgcacag ccgcgtggac cagctggact tctgggagag tggcgagtgg
     4441 gtcatcgtgg atgccgtggg cacctacaac accaggaagt acgagtgctg tgccgagatc
     4501 tacccggaca tcacctatgc cttcgtcatc cggcggctgc cgctcttcta caccatcaac
     4561 ctcatcatcc cctgcctgct catctcctgc ctcaccgtgc tggtcttcta cctgccctcc
     4621 gagtgtggcg agaagatcac gctgtgcatc tccgtgctgc tgtcgctcac cgtcttcctg
     4681 ctgctcatca ccgagatcat cccgtccacc tcactggtca tcccactcat cggcgagtac
     4741 ctgctgttca ccatgatctt cgtcaccctg tccatcgtca tcacggtctt cgtgctcaac
     4801 gtgcaccacc gctcgccacg cacgcacacc atgcccacct gggtacgcag ggtcttcctg
     4861 gacatcgtgc cacgcctgct cctcatgaag cggccgtccg tggtcaagga caattgccgg
     4921 cggctcatcg agtccatgca taagatggcc agtgccccgc gcttctggcc cgagccagaa
     4981 ggggagcccc ctgccacgag cggcacccag agcctgcacc cgccctcacc gtccttctgt
     5041 gtccccctgg atgtgccggc tgagcctggg ccttcctgca agtcaccctc cgaccagctc
     5101 cctcctcagc agcccctgga agctgagaaa gccagccccc acccctcgcc tggaccctgc
     5161 cgcccgcccc acggcaccca ggcaccaggg ctggccaaag ccaggtccct cagcgtccag
     5221 cacatgtcca gccctggcga agcggtggaa ggcggcgtcc ggtgccggtc tcggagcatc
     5281 cagtactgtg ttccccgaga cgatgccgcc cccgaggcag atggccaggc tgccggcgcc
     5341 ctggcctctc gcaacaccca ctcggctgag ctcccacccc cagaccagcc ctctccgtgc
     5401 aaatgcacat gcaagaagga gccctcttcg gtgtccccga gcgccacggt caagacccgc
     5461 agcaccaaag cgccgccccc gcacctgccc ctgtcgccgg ccctgacccg ggcggtggag
     5521 ggcgtccagt acattgcaga ccacctgaag gccgaagaca cagacttctc ggtgaaggag
     5581 gactggaagt acgtggccat ggtcatcgac cgcatcttcc tctggatgtt catcatcgtc
     5641 tgcctgctgg ggacggtggg cctcttcctg ccgccctggc tggctggcat gatccagcaa
     5701 cagcagcaac aacagcaaca gcagcaacag cagcaacaac agcaacagca gcaagctagc
     5761 acggatacag aggagcggct ggtggagcat ctcctggatc cttcccgcta caacaagctc
     5821 atccgcccag ccaccaatgg ctctgagctg gtgacagtac agcttatggt gtcactggcc
     5881 cagctcatca gtgtgcatga gcgggagcag atcatgacca ccaatgtctg gctgacccag
     5941 gagtgggaag attatcgcct cacctggaag cctgaagagt ttgacaacat gaagaaagtt
     6001 cggctccctt ccaaacacat ctggctccca gatgtggtcc tgtacaacaa tgctgacggc
     6061 atgtacgagg tgtccttcta ttccaatgcc gtggtctcct atgatggcag catcttctgg
     6121 ctgccgcctg ccatctacaa gagcgcatgc aagattgaag taaagcactt cccatttgac
     6181 cagcagaact gcaccatgaa gttccgttcg tggacctacg accgcacaga gatcgacttg
     6241 gtgctgaaga gtgaggtggc cagcctggac gacttcacac ctagtggtga gtgggacatc
     6301 gtggcgctgc cgggccggcg caacgagaac cccgacgact ctacgtacgt ggacatcacg
     6361 tatgacttca tcattcgccg caagccgctc ttctacacca tcaacctcat catcccctgt
     6421 gtgctcatca cctcgctggc catccttgtc ttctacctgc catccgactg tggcgagaag
     6481 atgacgttgt gcatctcagt gctgctggcg ctcacggtct tcctgctgct catctccaag
     6541 atcgtgcctc ccacctccct cgacgtgccg ctcgtcggca agtacctcat gttcaccatg
     6601 gtgcttgtca ccttctccat cgtcaccagc gtgtgcgtgc tcaacgtgca ccaccgctcg
     6661 cccaccacgc acaccatggc gccctgggtg aaggtcgtct tcctggagaa gctgcccgcg
     6721 ctgctcttca tgcagcagcc acgccatcat tgcgcccgtc agcgcctgcg cctgcggcga
     6781 cgccagcgtg agcgcgaggg cgctggagcc ctcttcttcc gcgaagcccc aggggccgac
     6841 tcctgcacgt gcttcgtcaa ccgcgcgtcg gtgcaggggt tggccggggc cttcggggct
     6901 gagcctgcac cagtggcggg ccccgggcgc tcaggggagc cgtgtggctg tggcctccgg
     6961 gaggcggtgg acggcgtgcg cttcatcgca gaccacatgc ggagcgagga cgatgaccag
     7021 agcgtgagtg aggactggaa gtacgtcgcc atggtgatcg accgcctctt cctctggatc
     7081 tttgtctttg tctgtgtctt tggcaccatc ggcatgttcc tgcagcctct cttccagaac
     7141 tacaccacca ccaccttcct ccactcagac cactcagccc ccagctccaa gcagcaacag
     7201 cagcaacaac agcaacagca gcaacagcag caacaactcg agcatgtgga gacccgggcc
     7261 cacgccgagg agcggctcct gaagaaactc ttctccggtt acaacaagtg gtcccgaccc
     7321 gtggccaaca tctcggacgt ggtcctcgtc cgcttcggcc tgtccatcgc tcagctcatt
     7381 gacgtggatg agaagaacca gatgatgacc acgaacgtat gggtgaagca ggagtggcac
     7441 gactacaagc tgcgctggga cccagctgac tatgagaatg tcacctccat ccgcatcccc
     7501 tccgagctca tctggcggcc ggacatcgtc ctctacaaca atgctgacgg ggacttcgcg
     7561 gtcacccacc tgaccaaggc ccacctgttc catgacgggc gggtgcagtg gactcccccg
     7621 gccatttaca agagctcctg cagcatcgac gtcaccttct tccccttcga ccagcagaac
     7681 tgcaccatga aattcggctc ctggacctac gacaaggcca agatcgacct ggtgaacatg
     7741 cacagccgcg tggaccagct ggacttctgg gagagtggcg agtgggtcat cgtggatgcc
     7801 gtgggcacct acaacaccag gaagtacgag tgctgtgccg agatctaccc ggacatcacc
     7861 tatgccttcg tcatccggcg gctgccgctc ttctacacca tcaacctcat catcccctgc
     7921 ctgctcatct cctgcctcac cgtgctggtc ttctacctgc cctccgagtg tggcgagaag
     7981 atcacgctgt gcatctccgt gctgctgtcg ctcaccgtct tcctgctgct catcaccgag
     8041 atcatcccgt ccacctcact ggtcatccca ctcatcggcg agtacctgct gttcaccatg
     8101 atcttcgtca ccctgtccat cgtcatcacg gtcttcgtgc tcaacgtgca ccaccgctcg
     8161 ccacgcacgc acaccatgcc cacctgggta cgcagggtct tcctggacat cgtgccacgc
     8221 ctgctcctca tgaagcggcc gtccgtggtc aaggacaatt gccggcggct catcgagtcc
     8281 atgcataaga tggccagtgc cccgcgcttc tggcccgagc cagaagggga gccccctgcc
     8341 acgagcggca cccagagcct gcacccgccc tcaccgtcct tctgtgtccc cctggatgtg
     8401 ccggctgagc ctgggccttc ctgcaagtca ccctccgacc agctccctcc tcagcagccc
     8461 ctggaagctg agaaagccag cccccacccc tcgcctggac cctgccgccc gccccacggc
     8521 acccaggcac cagggctggc caaagccagg tccctcagcg tccagcacat gtccagccct
     8581 ggcgaagcgg tggaaggcgg cgtccggtgc cggtctcgga gcatccagta ctgtgttccc
     8641 cgagacgatg ccgcccccga ggcagatggc caggctgccg gcgccctggc ctctcgcaac
     8701 acccactcgg ctgagctccc acccccagac cagccctctc cgtgcaaatg cacatgcaag
     8761 aaggagccct cttcggtgtc cccgagcgcc acggtcaaga cccgcagcac caaagcgccg
     8821 cccccgcacc tgcccctgtc gccggccctg acccgggcgg tggagggcgt ccagtacatt
     8881 gcagaccacc tgaaggccga agacacagac ttctcggtga aggaggactg gaagtacgtg
     8941 gccatggtca tcgaccgcat cttcctctgg atgttcatca tcgtctgcct gctggggacg
     9001 gtgggcctct tcctgccgcc ctggctggct ggcatgatct agaagcttgg ccgccatggc
     9061 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     9121 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     9181 cttatcatgt ctggatcggg aattaattcg gcgcagcacc atggcctgaa ataacctctg
     9241 aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     9301 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     9361 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9421 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     9481 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     9541 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     9601 ttggaggcct aggcttttgc aaaaagctgt taacagcttg gcactggccg tcgttttaca
     9661 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     9721 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     9781 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     9841 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     9901 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     9961 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gccttacaga caagctgtga
    10021 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
    10081 agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
    10141 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
    10201 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
    10261 tgttcatggg ggtaatgata ccgatgaaac ggccggcttt ccccgtcaag ctctaaatcg
    10321 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    10381 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    10441 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    10501 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    10561 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
    10621 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
    10681 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
    10741 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
    10801 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
    10861 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
    10921 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
    10981 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
    11041 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
    11101 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
    11161 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
    11221 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
    11281 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
    11341 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
    11401 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
    11461 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    11521 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
    11581 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    11641 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    11701 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    11761 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    11821 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    11881 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    11941 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    12001 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    12061 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    12121 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    12181 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    12241 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    12301 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    12361 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    12421 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    12481 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    12541 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    12601 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    12661 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    12721 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    12781 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    12841 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    12901 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    12961 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    13021 cagctatgac atgattacga attaa
//
