LOCUS       sequence-314823-       11047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11047)
  AUTHORS   Banta AB, Ward RD, Tran JS, Bacon EE, Peters JM
  TITLE     Programmable Gene Knockdown in Diverse Bacteria Using 
            Mobile-CRISPRi.
  JOURNAL   Curr Protoc Microbiol. 2020 Dec;59(1):e130. doi: 10.1002/cpmc.130.
  PUBMED    33332762
REFERENCE   2  (bases 1 to 11047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Microbiol."; date: "2020-12"; volume: "59(1):e130. doi"; pages: " 
            10.1002/cpmc.130"
COMMENT     Sequence Label: pJMP2782
FEATURES             Location/Qualifiers
     source          1..11047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..78
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             79..1161
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(98..117)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    1446..1462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    complement(1884..1931)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(2048..2053,2054..2070,2071..2076)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2265..2798
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     protein_bind    complement(2846..2893)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(2944..3030)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(3133..3227)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(3255..3271)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3256..3272)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3686..3705)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3805..3827
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3865..3883)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3951..4055
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4056..4124,4125..4916)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4274..4293)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5217..5605
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            5874..5982
                     /note="incP origin of transfer"
     mobile_element  6086..6251
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(6406..6495)
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             complement(join(6505..8088,8089..8091,8092..10578,
                     10579..10581,10582..10608))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    10650..10666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt
       61 caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
      121 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
      181 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
      241 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
      301 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
      361 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
      421 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
      481 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
      541 cccatcaaca gtattatttt ctcccatgaa gatggtacgc gactgggcgt ggagcatctg
      601 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
      661 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
      721 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
      781 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
      841 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
      901 gataccgaag atagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
      961 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
     1021 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
     1081 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     1141 cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc
     1201 accccacccg ggtccttgag ccccattatc acctccaaag ccttctcgtc tggtcagttt
     1261 cacctgtttt acgtaaaaac ccgcttcggc gggtttttac ttttggggaa acacagaaaa
     1321 aagcccgcac ctgacagtgc gggctttttt tttcgaccaa aggtgcgact actcttgcct
     1381 actacctatc gactgagctg aaagaattcc taaagatcta taaatgtgag cggataacat
     1441 tgacattgtg agcggataac aagatacttc tagttgagac caactttggt ctccaccata
     1501 gcggtcggtc tctgtttaag agctatgctg gaaacagcat agcaagttta aataaggcta
     1561 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttttga attcatgtgg
     1621 ctgaccgttc tgttgtctct cgctcttccg agtagacgaa caataaggcc tccctaacgg
     1681 ggggcctttt ttattgataa caaaagtcag tgcttccgct atttccaaaa taccgggcta
     1741 atacggttta aacgacctcc tggatttgct cagacagcct tttcgtcatt cgtttcagcc
     1801 aaaaaactta agaccgccgg tcttgtccac taccttgcag taatgcggtg gacaggatcg
     1861 gcggttttct tttctcttct caagaagttc ctatactttc tagagaatag gaacttcgga
     1921 ataggaactt cctcctgaac ggccataaga acgaaggctg tctgttgaac tctcgagcct
     1981 tgtcgccttg cgtataatat ttgcccatgg acgcacaccg tggaaacgga tgaaggcacg
     2041 aacccagttg acataagcct gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc
     2101 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
     2161 catggcttgt tatgactgtt tttttgtaca gtctatgcct cgggcatcca agcagcaagc
     2221 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcagca
     2281 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aggtggctca agtatgggca
     2341 tcattcgcac atgtaggctc ggccctgacc aagtcaaatc catgcgggct gctcttgatc
     2401 ttttcggtcg tgagttcgga gacgtagcca cctactccca acatcagccg gactccgatt
     2461 acctcgggaa cttgctccgt agtaagacat tcatcgcgct tgctgccttc gaccaagaag
     2521 cggttgttgg cgctctcgcg gcttacgttc tgcccaagtt tgagcagccg cgtagtgaga
     2581 tctatatcta tgatctcgca gtctccggag agcaccggag gcagggcatt gccaccgcgc
     2641 tcatcaatct cctcaagcat gaggccaacg cgcttggtgc ttatgtgatc tacgtgcaag
     2701 cagattacgg tgacgatccc gcagtggctc tctatacaaa gttgggcata cgggaagaag
     2761 tgatgcactt tgatatcgac ccaagtaccg ccacctaaca attcgttcaa gccgagatct
     2821 cgagcatcag cttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg
     2881 gaataggtac ttcaagatcc ccaattcgag ctcatcgtcc gggccgcaag ctcctagcgg
     2941 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     3001 gtctttcgac tgagcctttc gttttatttg atgcctcaag ctagagagtc attaccccag
     3061 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     3121 agtctagctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     3181 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gctcagctaa
     3241 ttaagctagc ttatcgatac cgtcgacctc gaaccccacg cccctcttta atacgacggg
     3301 caatttgcac ttcagaaaat gaagagtttg ctttagccat aacaaaagtc cagtatgctt
     3361 tttcacagca taactggact gatttcagtt tacaactatt ctgtctagtt taagacttta
     3421 ttgtcatagt ttagatctat tttgttcagt ttaagacttt attgtccgcc cacacctgct
     3481 tacgcagggc atccatttat tactcaaccg taaccgattt tgccaggtta cgcggctggt
     3541 cctctagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     3601 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3661 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     3721 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     3781 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcgt
     3841 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     3901 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3961 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4021 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4081 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4141 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4201 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4261 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4321 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4381 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4441 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4501 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4561 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     4621 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     4681 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     4741 ttgctgataa atctggagcc ggtgagcgtg ggtcccgcgg tatcattgca gcactggggc
     4801 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     4861 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     4921 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     4981 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5041 cgttccactg agcgtcagac cccgtagaaa agatcaaagg cagttattgg tgcctcactg
     5101 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5161 cttcattttt aatttttgcg gccgcaagat ccggccacga tgcgtccggc gtagaggatc
     5221 tgaagatcag cagttcaacc tgttgatagt acgtactaag ctctcatgtt tcacgtacta
     5281 agctctcatg tttaacgtac taagctctca tgtttaacga actaaaccct catggctaac
     5341 gtactaagct ctcatggcta acgtactaag ctctcatgtt tcacgtacta agctctcatg
     5401 tttgaacaat aaaattaata taaatcagca acttaaatag cctctaaggt tttaagtttt
     5461 ataagaaaaa aaagaatata taaggctttt aaagctttta aggtttaacg gttgtggaca
     5521 acaagccagg gatgtaacgc actgagaagc ccttagagcc tctcaaagca attttgagtg
     5581 acacaggaac acttaacggc tgacatggga attagccatg gcatcacagt atcgtgatga
     5641 cagaggcagg gagtgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5701 tgttctttcc tgcgttatca ggggattcct taaggtatac tttccgctgc ataaccctgc
     5761 ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga
     5821 ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg
     5881 ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac
     5941 ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgataagctc tgataccgct
     6001 cgccgcagcc gaacgaccga gcgcagcggg tcagtgagcg aggaagcgga agagcccaaa
     6061 cataacagga agaaaaatgc cccgctgtgg gcggacaaaa tagttgggaa ctgggagggg
     6121 tggaaatgga gtttttaagg attatttagg gaagagtgac aaaatagatg ggaactgggt
     6181 gtagcgtcgt aagctaatac gaaaattaaa aatgacaaaa tagtttggaa ctagatttca
     6241 cttatctggt tcttgaggcg gttaaaagag ccgtactctt ctccgatgtc gactaggcca
     6301 tgatgctcat tctgtgggac caaaacgaaa aaacaccctt tcgggtgtct tttctggaat
     6361 ttggtaccga ggctgcaagt cccattaagg aggcgcgcct tattacagat cttcctcaga
     6421 gatgagcttc tgttccagat cttcttctga aatcaacttt tgttccagat cttcttcaga
     6481 gatgagtttc tgctccgcgg ccgcgtcacc tcctagctga ctcaaatcaa tgcgtgtttc
     6541 ataaagacca gtgatggatt gatggataag agtggcatct aaaacttctt ttgtagacgt
     6601 atatcgttta cgatcaattg ttgtatcaaa atatttaaaa gcagcgggag ctccaagatt
     6661 cgtcaacgta aataaatgaa taatattttc tgcttgttca cgtattggtt tgtctctatg
     6721 tttgttatat gcactaagaa ctttatctaa attggcatct gctaaaataa cacgcttaga
     6781 aaattcactg atttgctcaa taatctcatc taaataatgc ttatgctgct ccacaaacaa
     6841 ttgtttttgt tcgttatctt ctggactacc cttcaacttt tcataatgac tagctaaata
     6901 taaaaaattc acatatttgc ttggcagagc cagctcattt cctttttgta attctccggc
     6961 actagccagc atccgtttac gaccgttttc taactcaaaa agactatatt taggtagttt
     7021 aatgattaag tcttttttaa cttccttata tcctttagct tctaaaaagt caatcggatt
     7081 tttttcaaag gaacttcttt ccataattgt gatccctagt aactctttaa cggattttaa
     7141 cttcttcgat ttcccttttt ccaccttagc aaccactagg actgaataag ctaccgttgg
     7201 actatcaaaa ccaccatatt tttttggatc ccagtctttt ttacgagcaa taagcttgtc
     7261 cgaatttctt tttggtaaaa ttgactcctt ggagaatccg cctgtctgta cttctgtttt
     7321 cttgacaata ttgacttggg gcatggacaa tactttgcgc actgtggcaa aatctcgccc
     7381 tttatcccag acaatttctc cagtttcccc attagtttcg attagagggc gtttgcgaat
     7441 ctctccattt gcaagtgtaa tttctgtttt gaagaagttc atgatattag agtaaaagaa
     7501 atattttgcg gttgctttgc ctatttcttg ctcagactta gcaatcattt tacgaacatc
     7561 ataaacttta taatcaccat agacaaactc cgattcaagt tttggatatt tcttaatcaa
     7621 agcagttcca acgacggcat ttagatacgc atcatgggca tgatggtaat tgttaatctc
     7681 acgtacttta tagaattgga aatcttttcg gaagtcagaa actaatttag attttaaggt
     7741 aatcacttta acctctcgaa taagtttatc attttcatcg tatttagtat tcatgcgact
     7801 atccaaaatt tgtgccacat gcttagtgat ttggcgagtt tcaaccaatt ggcgtttgat
     7861 aaaaccagct ttatcaagtt cactcaaacc tccacgttca gctttcgtta aattatcaaa
     7921 cttacgttga gtgattaact tggcgtttag aagttgtctc caatagtttt tcatcttttt
     7981 gactacttct tcacttggaa cgttatccga tttaccacga tttttatcag aacgcgttaa
     8041 gaccttattg tctattgaat cgtctttaag gaaactttgt ggaacaatgg catcgacatc
     8101 ataatcactt aaacgattaa tatctaattc ttggtccaca tacatgtctc ttccattttg
     8161 gagataatag agatagagct tttcattttg caattgagta ttttcaacag gatgctcttt
     8221 aagaatctga cttcctaatt ctttgatacc ttcttcgatt cgtttcatac gctctcgcga
     8281 atttttctgg cccttttgag ttgtctgatt ttcacgtgcc atttcaataa cgatattttc
     8341 tggcttatgc cgccccatta ctttgaccaa ttcatcaaca acttttacag tctgtaaaat
     8401 acctttttta atagcagggc taccagctaa atttgcaata tgttcatgta aactatcgcc
     8461 ttgtccagac acttgtgctt tttgaatatc ttctttaaat gtcaaactat catcatggat
     8521 cagctgcata aaattgcgat tggcaaaacc atctgatttc aaaaaatcta atattgtttt
     8581 gccagattgc ttatccctaa taccattaat caattttcga gacaaacgtc cccaaccagt
     8641 ataacggcga cgtttaagct gtttcatcac cttatcatca aagaggtgag catatgtttt
     8701 aagtctttcc tcaatcatct ccctatcttc aaataaggtc aatgttaaaa caatatcctc
     8761 taagatatct tcattttctt cattatccaa aaaatcttta tctttaataa tttttagcaa
     8821 atcatggtag gtacctaatg aagcattaaa tctatcttca actcctgaaa tttcaacact
     8881 atcaaaacat tctatttttt tgaaataatc ttcttttaat tgcttaacgg ttacttttcg
     8941 atttgttttg aagagtaaat caacaatggc tttcttctgt tcacctgaaa gaaatgctgg
     9001 ttttcgcatt ccttcagtaa catatttgac ctttgtcaat tcgttataaa ccgtaaaata
     9061 ctcataaagc aaactatgtt ttggtagtac tttttcattt ggaagatttt tatcaaagtt
     9121 tgtcatgcgt tcaataaatg attgagctga agcaccttta tcgacaactt cttcaaaatt
     9181 ccatggggta attgtttctt cagacttccg agtcatccat gcaaaacgac tattgccacg
     9241 cgccaatgga ccaacataat aagggattcg aaaagtcaag attttttcaa tcttctcacg
     9301 attgtctttt aaaaatggat aaaaatcttc ttgtcgtctc aaaatagcat gcagctcacc
     9361 caagtgaatt tgatggggaa tagagccgtt gtcaaaggtc cgttgcttgc gcagcaaatc
     9421 ttcacgattt agtttcacca ataattcctc agtaccatcc attttttcta aaattggttt
     9481 gataaattta taaaattctt cttggctagc tcccccatca atataacctg catatccgtt
     9541 ttttgattga tcaaaaaaga tttctttata cttttctgga agttgttgtc gaactaaagc
     9601 ttttaaaaga gtcaagtctt gatgatgttc atcgtagcgt ttaatcattg aagctgatag
     9661 gggagcctta gttatttcag tatttactct taggatatct gaaagtaaaa tagcatctga
     9721 taaattctta gctgccaaaa acaaatcagc atattgatct ccaatttgcg ccaataaatt
     9781 atctaaatca tcatcgtaag tatcttttga aagctgtaat ttagcatctt ctgccaaatc
     9841 aaaatttgat ttaaaattag gggtcaaacc caatgacaaa gcaatgagat tcccaaataa
     9901 gccatttttc ttctcaccgg ggagctgagc aatgagattt tctaatcgtc ttgatttact
     9961 caatcgtgca gaaagaatcg ctttagcatc tactccactt gcgttaatag ggttttcttc
    10021 aaataattga ttgtaggttt gtaccaactg gataaatagt ttgtccacat cactattatc
    10081 aggatttaaa tctccctcaa tcaaaaaatg accacgaaac ttaatcatat gcgctaaggc
    10141 caaatagatt aagcgcaaat ccgctttatc agtagaatct accaattttt ttcgcagatg
    10201 atagatagtt ggatatttct catgataagc aacttcatct actatatttc caaaaatagg
    10261 atgacgttca tgcttcttat cttcttccac caaaaaagac tcttcaagtc gatgaaagaa
    10321 actatcatct actttcgcca tctcatttga aaaaatctcc tgtagataac aaatacgatt
    10381 cttccgacgt gtataccttc tacgagctgt ccgtttgaga cgagtcgctt ccgctgtctc
    10441 tccactgtca aataaaagag cccctataag attttttttg atactgtggc ggtctgtatt
    10501 tcccagaacc ttgaactttt tagacggaac cttatattca tcagtgatca ccgcccatcc
    10561 gacgctattt gtgccgatag ctaagcctat tgagtatttc ttatccatac tagttttcct
    10621 gtgtgaaacc tgctgatgtg ctcagtatct tgttatccgc tcacaatgtc aatgttatcc
    10681 gctcacattt ataatatttt atctgattaa taagatgatc ttcttgagat cgttttggtc
    10741 tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg
    10801 ttctctgagc taccaactct ttgaaccgag gtaactggct tggtgataag ctgtcaaacc
    10861 agatcaattc gcactagtct gcagacgtaa aaaaagcggc gtggttagcc gcttttttaa
    10921 ttgccggagc gcaataaaaa agcccccgga aggtgatctt ccgggggctt tctcatgcgt
    10981 tgcttgtatt gaaaatgggg gccatgccta cctatctgcc tcccgggaag cggcatgcat
    11041 ttacgtt
//
