LOCUS       sequence-314825-       11828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mobile CRISPRi sfGFP 'test', empty sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11828)
  AUTHORS   Banta AB, Ward RD, Tran JS, Bacon EE, Peters JM
  TITLE     Programmable Gene Knockdown in Diverse Bacteria Using 
            Mobile-CRISPRi.
  JOURNAL   Curr Protoc Microbiol. 2020 Dec;59(1):e130. doi: 10.1002/cpmc.130.
  PUBMED    33332762
REFERENCE   2  (bases 1 to 11828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Microbiol."; date: "2020-12"; volume: "59(1):e130. doi"; pages: " 
            10.1002/cpmc.130"
COMMENT     Sequence Label: pJMP2820
FEATURES             Location/Qualifiers
     source          1..11828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..78
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             79..1161
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(98..117)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    1446..1462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(1837..1839,1840..2553)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1837..2550
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1867..1895)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2490..2510
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(2695..2742)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(2819..2824,2825..2841,2842..2847)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2930..3589
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2996..3015)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    complement(3627..3674)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(3725..3811)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(3914..4008)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(4036..4052)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4037..4053)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4467..4486)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4586..4608
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4646..4664)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4732..4836
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4837..4905,4906..5697)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5055..5074)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5998..6386
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            6655..6763
                     /note="incP origin of transfer"
     mobile_element  6867..7032
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(7187..7276)
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             complement(join(7286..8869,8870..8872,8873..11359,
                     11360..11362,11363..11389))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    11431..11447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt
       61 caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
      121 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
      181 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
      241 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
      301 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
      361 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
      421 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
      481 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
      541 cccatcaaca gtattatttt ctcccatgaa gatggtacgc gactgggcgt ggagcatctg
      601 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
      661 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
      721 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
      781 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
      841 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
      901 gataccgaag atagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
      961 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
     1021 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
     1081 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     1141 cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc
     1201 accccacccg ggtccttgag ccccattatc acctccaaag ccttctcgtc tggtcagttt
     1261 cacctgtttt acgtaaaaac ccgcttcggc gggtttttac ttttggggaa acacagaaaa
     1321 aagcccgcac ctgacagtgc gggctttttt tttcgaccaa aggtgcgact actcttgcct
     1381 actacctatc gactgagctg aaagaattcc taaagatcta taaatgtgag cggataacat
     1441 tgacattgtg agcggataac aagatacttc tagttgagac caactttggt ctccaccata
     1501 gcggtcggtc tctgtttaag agctatgctg gaaacagcat agcaagttta aataaggcta
     1561 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttttga attcatgtgg
     1621 ctgaccgttc tgttgtctct cgctcttccg agtagacgaa caataaggcc tccctaacgg
     1681 ggggcctttt ttattgataa caaaagtcag tgcttccgct atttccaaaa taccgggcta
     1741 atacggttta aacgaaaatt tatcaaaaag agtattgact taaagtctaa cctataggat
     1801 acttacagcc agatctgagc gagaaggagg taaagtatga gcaaaggaga agaacttttc
     1861 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     1921 gtccgtggag agggtgaagg tgatgctaca aacggaaaac tcacccttaa atttatttgc
     1981 actactggaa aactacctgt tccgtggcca acacttgtca ctactctgac ctatggtgtt
     2041 caatgctttt cccgttatcc ggatcacatg aaacggcatg actttttcaa gagtgccatg
     2101 cccgaaggtt atgtacagga acgcactata tctttcaaag atgacgggac ctacaagacg
     2161 cgtgctgaag tcaagtttga aggtgatacc cttgttaatc gtatcgagtt aaagggtatt
     2221 gattttaaag aagatggaaa cattcttgga cacaaactgg agtacaactt taactcacac
     2281 aatgtataca tcacggcaga caaacaaaag aatggaatca aagctaactt caaaattcgc
     2341 cacaacgttg aagatggttc cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     2401 ggcgatggcc ctgtcctttt accagacaac cattacctgt cgacacaatc tgtcctttcg
     2461 aaagatccca acgaaaagcg tgaccacatg gtccttcttg agtttgtaac tgctgctggg
     2521 attacacatg gcatggatga gctctacaaa taataagttt aaacgacctc ctggatttgc
     2581 tcagacagcc ttttcgtcat tcgtttcagc caaaaaactt aagaccgccg gtcttgtcca
     2641 ctaccttgca gtaatgcggt ggacaggatc ggcggttttc ttttctcttc tcaagaagtt
     2701 cctatacttt ctagagaata ggaacttcgg aataggaact tcctcctgaa cggccataag
     2761 aacgaaggct gtctgttgaa ctctcgagcc gtggaaacgg atgaaggcac gaacccagtt
     2821 gacataagcc tgttcggttc gtaaactgta atgcaagtag cgtatgcgct catcactacc
     2881 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     2941 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     3001 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     3061 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     3121 ccgcctgatg aatgctcatc cggagttccg tatggcaatg aaagacggtg agctggtgat
     3181 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     3241 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     3301 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     3361 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     3421 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     3481 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
     3541 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat cgactggaac
     3601 tcgagcatca gcttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc
     3661 ggaataggta cttcaagatc cccaattcga gctcatcgtc cgggccgcaa gctcctagcg
     3721 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3781 agtctttcga ctgagccttt cgttttattt gatgcctcaa gctagagagt cattacccca
     3841 ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg
     3901 cagtctagct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
     3961 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agctcagcta
     4021 attaagctag cttatcgata ccgtcgacct cgaaccccac gcccctcttt aatacgacgg
     4081 gcaatttgca cttcagaaaa tgaagagttt gctttagcca taacaaaagt ccagtatgct
     4141 ttttcacagc ataactggac tgatttcagt ttacaactat tctgtctagt ttaagacttt
     4201 attgtcatag tttagatcta ttttgttcag tttaagactt tattgtccgc ccacacctgc
     4261 ttacgcaggg catccattta ttactcaacc gtaaccgatt ttgccaggtt acgcggctgg
     4321 tcctctagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4381 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4441 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     4501 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4561 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtcg
     4621 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4681 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4741 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4801 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4861 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4921 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4981 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5041 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5101 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5161 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5221 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5281 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5341 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5401 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5461 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5521 attgctgata aatctggagc cggtgagcgt gggtcccgcg gtatcattgc agcactgggg
     5581 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5641 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5701 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5761 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5821 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gcagttattg gtgcctcact
     5881 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5941 acttcatttt taatttttgc ggccgcaaga tccggccacg atgcgtccgg cgtagaggat
     6001 ctgaagatca gcagttcaac ctgttgatag tacgtactaa gctctcatgt ttcacgtact
     6061 aagctctcat gtttaacgta ctaagctctc atgtttaacg aactaaaccc tcatggctaa
     6121 cgtactaagc tctcatggct aacgtactaa gctctcatgt ttcacgtact aagctctcat
     6181 gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg ttttaagttt
     6241 tataagaaaa aaaagaatat ataaggcttt taaagctttt aaggtttaac ggttgtggac
     6301 aacaagccag ggatgtaacg cactgagaag cccttagagc ctctcaaagc aattttgagt
     6361 gacacaggaa cacttaacgg ctgacatggg aattagccat ggcatcacag tatcgtgatg
     6421 acagaggcag ggagtgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6481 atgttctttc ctgcgttatc aggggattcc ttaaggtata ctttccgctg cataaccctg
     6541 cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg
     6601 attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag
     6661 gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca
     6721 cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgataagct ctgataccgc
     6781 tcgccgcagc cgaacgaccg agcgcagcgg gtcagtgagc gaggaagcgg aagagcccaa
     6841 acataacagg aagaaaaatg ccccgctgtg ggcggacaaa atagttggga actgggaggg
     6901 gtggaaatgg agtttttaag gattatttag ggaagagtga caaaatagat gggaactggg
     6961 tgtagcgtcg taagctaata cgaaaattaa aaatgacaaa atagtttgga actagatttc
     7021 acttatctgg ttcttgaggc ggttaaaaga gccgtactct tctccgatgt cgactaggcc
     7081 atgatgctca ttctgtggga ccaaaacgaa aaaacaccct ttcgggtgtc ttttctggaa
     7141 tttggtaccg aggctgcaag tcccattaag gaggcgcgcc ttattacaga tcttcctcag
     7201 agatgagctt ctgttccaga tcttcttctg aaatcaactt ttgttccaga tcttcttcag
     7261 agatgagttt ctgctccgcg gccgcgtcac ctcctagctg actcaaatca atgcgtgttt
     7321 cataaagacc agtgatggat tgatggataa gagtggcatc taaaacttct tttgtagacg
     7381 tatatcgttt acgatcaatt gttgtatcaa aatatttaaa agcagcggga gctccaagat
     7441 tcgtcaacgt aaataaatga ataatatttt ctgcttgttc acgtattggt ttgtctctat
     7501 gtttgttata tgcactaaga actttatcta aattggcatc tgctaaaata acacgcttag
     7561 aaaattcact gatttgctca ataatctcat ctaaataatg cttatgctgc tccacaaaca
     7621 attgtttttg ttcgttatct tctggactac ccttcaactt ttcataatga ctagctaaat
     7681 ataaaaaatt cacatatttg cttggcagag ccagctcatt tcctttttgt aattctccgg
     7741 cactagccag catccgttta cgaccgtttt ctaactcaaa aagactatat ttaggtagtt
     7801 taatgattaa gtctttttta acttccttat atcctttagc ttctaaaaag tcaatcggat
     7861 ttttttcaaa ggaacttctt tccataattg tgatccctag taactcttta acggatttta
     7921 acttcttcga tttccctttt tccaccttag caaccactag gactgaataa gctaccgttg
     7981 gactatcaaa accaccatat ttttttggat cccagtcttt tttacgagca ataagcttgt
     8041 ccgaatttct ttttggtaaa attgactcct tggagaatcc gcctgtctgt acttctgttt
     8101 tcttgacaat attgacttgg ggcatggaca atactttgcg cactgtggca aaatctcgcc
     8161 ctttatccca gacaatttct ccagtttccc cattagtttc gattagaggg cgtttgcgaa
     8221 tctctccatt tgcaagtgta atttctgttt tgaagaagtt catgatatta gagtaaaaga
     8281 aatattttgc ggttgctttg cctatttctt gctcagactt agcaatcatt ttacgaacat
     8341 cataaacttt ataatcacca tagacaaact ccgattcaag ttttggatat ttcttaatca
     8401 aagcagttcc aacgacggca tttagatacg catcatgggc atgatggtaa ttgttaatct
     8461 cacgtacttt atagaattgg aaatcttttc ggaagtcaga aactaattta gattttaagg
     8521 taatcacttt aacctctcga ataagtttat cattttcatc gtatttagta ttcatgcgac
     8581 tatccaaaat ttgtgccaca tgcttagtga tttggcgagt ttcaaccaat tggcgtttga
     8641 taaaaccagc tttatcaagt tcactcaaac ctccacgttc agctttcgtt aaattatcaa
     8701 acttacgttg agtgattaac ttggcgttta gaagttgtct ccaatagttt ttcatctttt
     8761 tgactacttc ttcacttgga acgttatccg atttaccacg atttttatca gaacgcgtta
     8821 agaccttatt gtctattgaa tcgtctttaa ggaaactttg tggaacaatg gcatcgacat
     8881 cataatcact taaacgatta atatctaatt cttggtccac atacatgtct cttccatttt
     8941 ggagataata gagatagagc ttttcatttt gcaattgagt attttcaaca ggatgctctt
     9001 taagaatctg acttcctaat tctttgatac cttcttcgat tcgtttcata cgctctcgcg
     9061 aatttttctg gcccttttga gttgtctgat tttcacgtgc catttcaata acgatatttt
     9121 ctggcttatg ccgccccatt actttgacca attcatcaac aacttttaca gtctgtaaaa
     9181 tacctttttt aatagcaggg ctaccagcta aatttgcaat atgttcatgt aaactatcgc
     9241 cttgtccaga cacttgtgct ttttgaatat cttctttaaa tgtcaaacta tcatcatgga
     9301 tcagctgcat aaaattgcga ttggcaaaac catctgattt caaaaaatct aatattgttt
     9361 tgccagattg cttatcccta ataccattaa tcaattttcg agacaaacgt ccccaaccag
     9421 tataacggcg acgtttaagc tgtttcatca ccttatcatc aaagaggtga gcatatgttt
     9481 taagtctttc ctcaatcatc tccctatctt caaataaggt caatgttaaa acaatatcct
     9541 ctaagatatc ttcattttct tcattatcca aaaaatcttt atctttaata atttttagca
     9601 aatcatggta ggtacctaat gaagcattaa atctatcttc aactcctgaa atttcaacac
     9661 tatcaaaaca ttctattttt ttgaaataat cttcttttaa ttgcttaacg gttacttttc
     9721 gatttgtttt gaagagtaaa tcaacaatgg ctttcttctg ttcacctgaa agaaatgctg
     9781 gttttcgcat tccttcagta acatatttga cctttgtcaa ttcgttataa accgtaaaat
     9841 actcataaag caaactatgt tttggtagta ctttttcatt tggaagattt ttatcaaagt
     9901 ttgtcatgcg ttcaataaat gattgagctg aagcaccttt atcgacaact tcttcaaaat
     9961 tccatggggt aattgtttct tcagacttcc gagtcatcca tgcaaaacga ctattgccac
    10021 gcgccaatgg accaacataa taagggattc gaaaagtcaa gattttttca atcttctcac
    10081 gattgtcttt taaaaatgga taaaaatctt cttgtcgtct caaaatagca tgcagctcac
    10141 ccaagtgaat ttgatgggga atagagccgt tgtcaaaggt ccgttgcttg cgcagcaaat
    10201 cttcacgatt tagtttcacc aataattcct cagtaccatc cattttttct aaaattggtt
    10261 tgataaattt ataaaattct tcttggctag ctcccccatc aatataacct gcatatccgt
    10321 tttttgattg atcaaaaaag atttctttat acttttctgg aagttgttgt cgaactaaag
    10381 cttttaaaag agtcaagtct tgatgatgtt catcgtagcg tttaatcatt gaagctgata
    10441 ggggagcctt agttatttca gtatttactc ttaggatatc tgaaagtaaa atagcatctg
    10501 ataaattctt agctgccaaa aacaaatcag catattgatc tccaatttgc gccaataaat
    10561 tatctaaatc atcatcgtaa gtatcttttg aaagctgtaa tttagcatct tctgccaaat
    10621 caaaatttga tttaaaatta ggggtcaaac ccaatgacaa agcaatgaga ttcccaaata
    10681 agccattttt cttctcaccg gggagctgag caatgagatt ttctaatcgt cttgatttac
    10741 tcaatcgtgc agaaagaatc gctttagcat ctactccact tgcgttaata gggttttctt
    10801 caaataattg attgtaggtt tgtaccaact ggataaatag tttgtccaca tcactattat
    10861 caggatttaa atctccctca atcaaaaaat gaccacgaaa cttaatcata tgcgctaagg
    10921 ccaaatagat taagcgcaaa tccgctttat cagtagaatc taccaatttt tttcgcagat
    10981 gatagatagt tggatatttc tcatgataag caacttcatc tactatattt ccaaaaatag
    11041 gatgacgttc atgcttctta tcttcttcca ccaaaaaaga ctcttcaagt cgatgaaaga
    11101 aactatcatc tactttcgcc atctcatttg aaaaaatctc ctgtagataa caaatacgat
    11161 tcttccgacg tgtatacctt ctacgagctg tccgtttgag acgagtcgct tccgctgtct
    11221 ctccactgtc aaataaaaga gcccctataa gatttttttt gatactgtgg cggtctgtat
    11281 ttcccagaac cttgaacttt ttagacggaa ccttatattc atcagtgatc accgcccatc
    11341 cgacgctatt tgtgccgata gctaagccta ttgagtattt cttatccata ctagttttcc
    11401 tgtgtgaaac ctgctgatgt gctcagtatc ttgttatccg ctcacaatgt caatgttatc
    11461 cgctcacatt tataatattt tatctgatta ataagatgat cttcttgaga tcgttttggt
    11521 ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct tgcagggcgg tttttcgaag
    11581 gttctctgag ctaccaactc tttgaaccga ggtaactggc ttggtgataa gctgtcaaac
    11641 cagatcaatt cgcactagtc tgcagacgta aaaaaagcgg cgtggttagc cgctttttta
    11701 attgccggag cgcaataaaa aagcccccgg aaggtgatct tccgggggct ttctcatgcg
    11761 ttgcttgtat tgaaaatggg ggccatgcct acctatctgc ctcccgggaa gcggcatgca
    11821 tttacgtt
//
