LOCUS       sequence-31483-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AD-003
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..97
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             793..810
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(863..881)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ccctctgaat attttgttta ctttaagaag gagatatacc atgggcagca gccatcatca
       61 tcatcatcac agcagcggcc tggttccgcg tggtagtacg agcgaggtga tagaagacga
      121 gaagcaattc tattccaagg ccaagaccta ctggaaacaa atcccaccca cggtggacgg
      181 catgcttggg gggtatggcc acatctccag catcgacatc aacagctccc ggaagtttct
      241 gcagaggttt ttgagggaag gcccgaacaa gacaggaacg tcctgtgccc tggactgtgg
      301 agctggcatt gggaggatca ccaagcggct gctcctgccg ctgttcagag aggtggatat
      361 ggtcgacata acggaggact tcctggttca agccaagacc tacctggggg aggagggcaa
      421 gagggtgagg aactacttct gttgtgggct ccaggacttc accccggagc cggactctta
      481 cgacgtgatc tggatccagt gggtgatagg ccacctcacc gatcagcacc tggccgagtt
      541 cctgcggcgc tgcaagggca gcctccgccc caacggcatc atcgtcatca aagacaacat
      601 ggcccaggag ggcgtgattc tggacgacgt ggacagcagc gtgtgccggg accttgacgt
      661 ggtccgcagg atcatctgca gtgcaggcct cagcctcctg gccgaggaga ggcaggagaa
      721 cctccccgat gagatctacc atgtctatag ctttgccctg agatgatgac gaagcttgcg
      781 gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
      841 gaagctgagt tggctgctgc caccgctgag caataactag cata
//
