LOCUS       sequence-314833-        5144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of c-luciferase from sp. Kornikeria hastingsi carriebowae
            (Belize).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Hensley NM, Ellis EA, Leung NY, Coupart J, Mikhailovsky A, Taketa 
            DA, Tessler M, Gruber DF, De Tomaso AW, Mitani Y, Rivers TJ, Gerrish
            GA, Torres E, Oakley TH
  TITLE     Selection, drift, and constraint in cypridinid luciferases and the 
            diversification of bioluminescent signals in sea fireflies.
  JOURNAL   Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.15673.
  PUBMED    33031624
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ecol. 
            2020 Oct 8. doi: 10.1111/mec.15673."
COMMENT     Sequence Label: pPICZ-aC-KHC-BZ
FEATURES             Location/Qualifiers
     source          1..5144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             join(941..997,998..1195)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKR"
     CDS             2825..2854
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2870..2887
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2967..3213
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        3228..3641
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        3648..3695
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3714..4088
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      4154..4401
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4170..4188)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(4476..5064)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4556..4575)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg atgagatttc cttcaatttt
      961 tactgctgtt ttattcgcag catcctccgc attagctgct ccagtcaaca ctacaacaga
     1021 agatgaaacg gcacaaattc cggctgaagc tgtcatcggt tactcagatt tagaagggga
     1081 tttcgatgtt gctgttttgc cattttccaa cagcacaaat aacgggttat tgtttataaa
     1141 tactactatt gccagcattg ctgctaaaga agaaggggta tctctcgaga agagagaggc
     1201 taaagattgt tttgaatcat ctttccacag cctagaattt ccttcgtcat gtgaggcaca
     1261 aaatggcatc tgtattgata gcgagtgtaa agactgtaat gaagttatgt ttggcgatgg
     1321 attgtgcgaa aatgccggag gagcctctcc aaagtgctgt agagattgtc cggaaattgt
     1381 tagatgcagg gcttcagcag ctgggttctt tacaacgttt tatggaaaga agttcaatct
     1441 tcaggaacca ggaacctatc tgctaagtga agactgcgtc ggaggcctgt ggtctttata
     1501 tgttacgctg gtgaatatag caggagaaaa gggtgctgtt ctgggatccg taaagatgat
     1561 tgttggagaa gtaacggtag atattgtaca gaaaggagga cccgtcacag taaatggagg
     1621 ttctgttgca atcgacagca atcctttctc tattggtgac gtcactattg ccatcgtaca
     1681 cacacctaac ttcgacgttg ctgttataga gtttcttaag ctggttacct ttgatatcat
     1741 ccatggaaga gctttccgac tagctcccga tttcctgtac cgagatagga cctgtggttt
     1801 gtgtggcgtg atgtcaaatg agccgactga ttttattgat aatccggatc agctagcagt
     1861 ccaagataag atcaacaaag atatagatgg ttgtcctctt tctggaaatc cttctgatgt
     1921 cgaatattgc aaaaataaga tgcagccata caaggtcgct tgtataaact acaatgacgt
     1981 caatttcgct acttacctat attcctgcgc cttggcctac tgtatgggcg gggaggacag
     2041 agtagaagac gtcatatttg attatgtaga agcatgcgta gaaccaataa cgagagctac
     2101 atgtgttatg aatggacaca catattatga cacattcgac aaaacaagtt atcaatttca
     2161 ggcaccatgc aaggttttat tcgctaaaga ctgcgcagga gatgaatggg aagtgacaat
     2221 tactcataaa gcggctggaa catacacaga agtagagaag gtcaccgtcc gatatttcca
     2281 aacgttaata gatttgatat cagaaggtaa acaggttctg gtaaacggaa cagaagtaag
     2341 tgtaccttac aataaaggag atacgtccat ctacatgtac gacaatctga taacaacagc
     2401 ggttctacca ggggcagtgg tcgtaaaata caactttgag caaatgcttg cgctgcacat
     2461 aagagatcca tacgaaagaa ggagctgtgg cttatgtgga atttgggatt tggataaatc
     2521 taacgatggt cctgacaacc agtatgtaga ctgtgaacca acccccaacc ctgcaacctg
     2581 cactgctgac caagaggcag aagccagaga actatgtcag aacatgtttc ctgcttcgct
     2641 cgatgatgaa tgtgacatct gctacaagtc tgaccgcgtg gagcggtgca tgtacgagta
     2701 ttgccttggt ggaatggatt acttctgtaa gcacgcaggt accgtcattg atgaatgctt
     2761 cgtgagacac ggagatgatc tacaattacc acctcaatgc accaaagcgg ccgccagctt
     2821 tctagaacaa aaactcatct cagaagagga tctgaatagc gccgtcgacc atcatcatca
     2881 tcatcattga gtttgtagcc ttagacatga ctgttcctca gttcaagttg ggcacttacg
     2941 agaagaccgg tcttgctaga ttctaatcaa gaggatgtca gaatgccatt tgcctgagag
     3001 atgcaggctt catttttgat acttttttat ttgtaaccta tatagtatag gatttttttt
     3061 gtcattttgt ttcttctcgt acgagcttgc tcctgatcag cctatctcgc agctgatgaa
     3121 tatcttgtgg taggggtttg ggaaaatcat tcgagtttga tgtttttctt ggtatttccc
     3181 actcctcttc agagtacaga agattaagtg agaccttcgt ttgtgcggat cccccacaca
     3241 ccatagcttc aaaatgtttc tactcctttt ttactcttcc agattttctc ggactccgcg
     3301 catcgccgta ccacttcaaa acacccaagc acagcatact aaatttcccc tctttcttcc
     3361 tctagggtgt cgttaattac ccgtactaaa ggtttggaaa agaaaaaaga gaccgcctcg
     3421 tttctttttc ttcgtcgaaa aaggcaataa aaatttttat cacgtttctt tttcttgaaa
     3481 attttttttt ttgatttttt tctctttcga tgacctccca ttgatattta agttaataaa
     3541 cggtcttcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa ctttttttac
     3601 ttcttgctca ttagaaagaa agcatagcaa tctaatctaa gggcggtgtt gacaattaat
     3661 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     3721 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     3781 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     3841 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     3901 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     3961 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     4021 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     4081 aggactgaca cgtccgacgc ggcccgacgg gtccgaggcc tcggagatcc gtcccccttt
     4141 tcctttgtcg atatcatgta attagttatg tcacgcttac attcacgccc tccccccaca
     4201 tccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt
     4261 tttatagtta tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg
     4321 tacagacgcg tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga
     4381 cgctcgaagg ctttaatttg caagctggag accaacatgt gagcaaaagg ccagcaaaag
     4441 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4501 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4561 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4621 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4681 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4741 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4801 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4861 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     4921 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4981 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5041 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5101 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gatc
//
