LOCUS       sequence-314834-        5281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of c-luciferase from sp. Vargula tsujii (USA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Hensley NM, Ellis EA, Leung NY, Coupart J, Mikhailovsky A, Taketa 
            DA, Tessler M, Gruber DF, De Tomaso AW, Mitani Y, Rivers TJ, Gerrish
            GA, Torres E, Oakley TH
  TITLE     Selection, drift, and constraint in cypridinid luciferases and the 
            diversification of bioluminescent signals in sea fireflies.
  JOURNAL   Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.15673.
  PUBMED    33031624
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ecol. 
            2020 Oct 8. doi: 10.1111/mec.15673."
COMMENT     Sequence Label: pPICZ-aC-VTS-US
FEATURES             Location/Qualifiers
     source          1..5281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             join(941..997,998..1195)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKR"
     CDS             2962..2991
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3007..3024
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3104..3350
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        3365..3778
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        3785..3832
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3851..4225
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      4291..4538
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4307..4325)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(4613..5201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4693..4712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg atgagatttc cttcaatttt
      961 tactgctgtt ttattcgcag catcctccgc attagctgct ccagtcaaca ctacaacaga
     1021 agatgaaacg gcacaaattc cggctgaagc tgtcatcggt tactcagatt tagaagggga
     1081 tttcgatgtt gctgttttgc cattttccaa cagcacaaat aacgggttat tgtttataaa
     1141 tactactatt gccagcattg ctgctaaaga agaaggggta tctctcgaga agagagaggc
     1201 tcaagattgt tatgaatcaa catgagcctc tctcagtatc ctcctcagtg taaagcggcc
     1261 gctttacact gaggaggata ctatcagtca tgttgattca taacaatctt gagcctctct
     1321 cttctcgaga agagagaggc tcaagattgt tatgaatcaa catgggcttc gaatgattat
     1381 ccatcttcat gtgaggctct aaacggaaga tgtgtagact ctgcgtgcgg cagctgtgac
     1441 gaggttctgt ttggtgatgg attgtgtgaa aatgcaggag gagcgtctcc caagtgctgt
     1501 agagattgcc cagaaatcgt cagatgcaga gtttcagcag ctggatactt ccaaacattc
     1561 tatgggcaga gattcaatct ccaggtacca ggaacctatc tcttgagtga agactgcgtg
     1621 ggaggactat ggtctctata cgttaacctg gcgaatattg aaggagagaa gggagcagtt
     1681 ttggattccg tcaagatggt tgttggagac gtaactgtcg atattaaaca gaaagttgga
     1741 gctgttacag taaatggagg ttccgttgaa atcgacagta acccattctc tattggtgac
     1801 gtcactattg ccatcgtaca cactcctaac tttgatgtct ccgtcatcga gttccttaag
     1861 cttgttacat tcgatatctt acaaggcaga gctttcagac ttgctccaga tttcttatac
     1921 gcagacagga cctgtggttt atgtggagtc atgtccgatg aacctactga ttttattgac
     1981 aatccagatc agcttgcaat ccaggaccaa atgaatcaag atatagacgg ctgcccactc
     2041 tctggaaatc cttctgacgt cgaatattgc gtgaataaga tgcagccata taaggacggg
     2101 tgtgttaaca agaacgatgt ccacttcgga acctacctgt acgcatgcgc actggcttac
     2161 tgcatgggcg gcgacgacag agtggaggat gttatatttg aatacgccga agcctgcgtt
     2221 gaacccatcg gtagagcaac ctgcgtcatg aatggacaca catactacga tacattcgac
     2281 aaaacaagtt atcagttcca ggcaccttgc aaggttctat tcgccaaaga ctgtacagat
     2341 gacgattggg aagtaaccat tactcataaa gcagttggag aatatactga agtagagaag
     2401 gtcaccgtcc ggtattttca aaccttgatt gacctgatat cggagactaa gaaagttttc
     2461 gttaatggaa cggaagtgag cgtcccttac aactatggag atacatctat atacatgtat
     2521 gataacttaa taactacagc agttctacca ggagcagtgg tggtgaaata caacttcgag
     2581 cagatgttgg ctctacacat cagagatcca tatgaagcag acagctgtgg tctgtgtggt
     2641 atctgggatt tggataaatc taatgagggt cctgataccc agtatgtaga ttgtgaacca
     2701 actcccaacc ctccaacatg tactgctgac aaagaggctg aagcaaggga actctgtcag
     2761 aatatgttcc ctgcttctct cgacgaccaa tgtaacatct gctacaaggc tgaccgtgtt
     2821 gagcgctgca tgtatgagta ttgtcttggt ggtctggagg gcttctgtca acacgctggt
     2881 accgtcatcg atgagtgctt cgtgagacac ggagacgatc tacagtatcc tcctcagtgt
     2941 aaagcggccg ccagctttct agaacaaaaa ctcatctcag aagaggatct gaatagcgcc
     3001 gtcgaccatc atcatcatca tcattgagtt tgtagcctta gacatgactg ttcctcagtt
     3061 caagttgggc acttacgaga agaccggtct tgctagattc taatcaagag gatgtcagaa
     3121 tgccatttgc ctgagagatg caggcttcat ttttgatact tttttatttg taacctatat
     3181 agtataggat tttttttgtc attttgtttc ttctcgtacg agcttgctcc tgatcagcct
     3241 atctcgcagc tgatgaatat cttgtggtag gggtttggga aaatcattcg agtttgatgt
     3301 ttttcttggt atttcccact cctcttcaga gtacagaaga ttaagtgaga ccttcgtttg
     3361 tgcggatccc ccacacacca tagcttcaaa atgtttctac tcctttttta ctcttccaga
     3421 ttttctcgga ctccgcgcat cgccgtacca cttcaaaaca cccaagcaca gcatactaaa
     3481 tttcccctct ttcttcctct agggtgtcgt taattacccg tactaaaggt ttggaaaaga
     3541 aaaaagagac cgcctcgttt ctttttcttc gtcgaaaaag gcaataaaaa tttttatcac
     3601 gtttcttttt cttgaaaatt tttttttttg atttttttct ctttcgatga cctcccattg
     3661 atatttaagt taataaacgg tcttcaattt ctcaagtttc agtttcattt ttcttgttct
     3721 attacaactt tttttacttc ttgctcatta gaaagaaagc atagcaatct aatctaaggg
     3781 cggtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     3841 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     3901 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     3961 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     4021 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     4081 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     4141 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     4201 cttcgtggcc gaggagcagg actgacacgt ccgacgcggc ccgacgggtc cgaggcctcg
     4261 gagatccgtc ccccttttcc tttgtcgata tcatgtaatt agttatgtca cgcttacatt
     4321 cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct
     4381 aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa
     4441 tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc
     4501 ttgagaaggt tttgggacgc tcgaaggctt taatttgcaa gctggagacc aacatgtgag
     4561 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4621 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4681 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4741 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4801 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4861 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4921 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4981 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5041 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5101 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5161 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5221 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5281 c
//
