LOCUS       sequence-314844-        1720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast display platform plasmid N3 which allows for simultaneous 
            expression of the protein of interest at the N-terminus of Aga2p 
            yeast agglutinin and eUnaG2 or nbALFA detection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Zahradnik J, Dey D, Marciano S, Schreiber G
  TITLE     An enhanced yeast display platform demonstrates the binding 
            plasticity under various selection pressures
  JOURNAL   bioRxiv Preprint 2020
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            Preprint 2020"
COMMENT     Sequence Label: pJYDN3
FEATURES             Location/Qualifiers
     source          1..1720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        182..623
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    182..299
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     594..617
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             745..783
                     /codon_start=1
                     /product="rationally designed epitope tag that forms a 
                     stable alpha-helix (Gotzke et al., 2019)"
                     /label=ALFA-tag
                     /note="It may be beneficial to flank the ALFA-tag sequence 
                     with proline residues to preserve the helical structure."
                     /translation="SRLEEELRRRLTE"
     CDS             1125..1136
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1137..1163
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1230..1259
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 acgccagcaa cgcggcactt tgtaactgag ctgtcatttt gctggcccag ctggtcgacc
       61 gcggctaagc agtactggta ccgttgtgga aatgtaaaga gccccattat cttagcctaa
      121 aaaaaccttc tctttggaac tttcagtaat acgcttaact gctcattgct atattgaagt
      181 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
      241 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      301 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      361 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      421 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      481 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      541 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      601 ctctatactt taacgtcaag gagaaaaaac cccggatcga attccctact tcatacattt
      661 tcaattaaga tgaggttccc atctattttc accgctgttg tttttgctgc ttcttctgct
      721 ttggctgctc cagctaatgg tccatccaga ttggaagaag aattgagaag aagattaacc
      781 gaaccacata tggaagcata ataattaagg cgccttatct cgaggggggc ggatcctctg
      841 gtggaggtgg ttcgggcgga ggtggtaacg gatctaatgg ttctggcggt agctctgcac
      901 aggaactgac aactatatgc gagcaaatcc cctcaccaac tttagaatcg acgccgtact
      961 ctttgtcaac gactactatt ttggccaacg ggaaggcaat gcaaggagtt tttgaatatt
     1021 acaaatcagt aacgtttgtc agtaattgcg gttctcaccc ctcaacaact agcaaaggca
     1081 gccccataaa cacacagtat gtttttaagg acaatagctc gacgattgaa ggtagatacc
     1141 catacgacgt tccagactac gctctgcagg ctagtggtgg aggaggctct ggtggaggtg
     1201 gtagcggagg cggagggtcg gctagccatg aacagaagtt gatctctgaa gaagatttga
     1261 tgttagaaaa atttgttggc acctggaaga tcgaatcctc tgaaaatttt ggtgaatact
     1321 tgaaggctat cggtgcccca aaagaattgg ctgatgctgg tgatgctact actccagtct
     1381 tgtacatttc tcaaaaggat ggtgataaga tgaccgtcaa gattgaaaac ggtccaccaa
     1441 cttttttgga tacccaagtt tctttcaagt tgggtgaaga attcgacgaa tttccatccg
     1501 atagaagaaa gggtgttaag tccgttgtta acttgtctgg tgagaagttg gtttacgttc
     1561 aaaagtggga tggtaaagaa accacttacg tcagagaaat caaggacggt aaattggttg
     1621 ttactttgac catgggtgat gttgttgctg ttagatctta tagaagggcc tctgaataat
     1681 agagatctga taacaacagt gtagatgtaa caaaatcgac
//
