LOCUS       sequence-314850-        1684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast display plasmid for expression of C-terminal fusions with 
            Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent 
            green-yellow fluorescent protein, is fused to the Nterminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Zahradnik J, Dey D, Marciano S, Schreiber G
  TITLE     An enhanced yeast display platform demonstrates the binding 
            plasticity under various selection pressures
  JOURNAL   bioRxiv Preprint 2020
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            Preprint 2020"
COMMENT     Sequence Label: pJYDC1
FEATURES             Location/Qualifiers
     source          1..1684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        179..620
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    179..296
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     591..614
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             1390..1401
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1402..1428
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1559..1588
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ccagcaacgc ggcactttgt aactgagctg tcattttgct ggcccagctg gtcgaccgcg
       61 gctaagcagt actggtaccg ttgtggaaat gtaaagagcc ccattatctt agcctaaaaa
      121 aaccttctct ttggaacttt cagtaatacg cttaactgct cattgctata ttgaagtacg
      181 gattagaagc cgccgagcgg gtgacagccc tccgaaggaa gactctcctc cgtgcgtcct
      241 cgtcttcacc ggtcgcgttc ctgaaacgca gatgtgcctc gcgccgcact gctccgaaca
      301 ataaagattc tacaatacta gcttttatgg ttatgaagag gaaaaattgg cagtaacctg
      361 gccccacaaa ccttcaaatg aacgaatcaa attaacaacc ataggatgat aatgcgatta
      421 gttttttagc cttatttctg gggtaattaa tcagcgaagc gatgattttt gatctattaa
      481 cagatatata aatgcaaaaa ctgcataacc actttaacta atactttcaa cattttcggt
      541 ttgtattact tcttattcaa atgtaataaa agtatcaaca aaaaattgtt aatatacctc
      601 tatactttaa cgtcaaggag aaaaaacccc ggatcgaatt ccctacttca tacattttca
      661 attaagatga ggttcccatc tattttcacc gctgttgttt ttgctgcttc ttctgctttg
      721 gctgctccag ctaatggtat gttagaaaaa tttgttggca cctggaagat cgaatcctct
      781 gaaaattttg gtgaatactt gaaggctatc ggtgccccaa aagaattggc tgatgctggt
      841 gatgctacta ctccagtctt gtacatttct caaaaggatg gtgataagat gaccgtcaag
      901 attgaaaacg gtccaccaac ttttttggat acccaagttt ctttcaagtt gggtgaagaa
      961 ttcgacgaat ttccatccga tagaagaaag ggtgttaagt ccgttgttaa cttgtctggt
     1021 gagaagttgg tttacgttca aaagtgggat ggtaaagaaa ccacttacgt cagagaaatc
     1081 aaggacggta aattggttgt tactttgacc atgggtgatg ttgttgctgt tagatcttat
     1141 agaagggcct ctgaagtttc tgcacaggaa ctgacaacta tatgcgagca aatcccctca
     1201 ccaactttag aatcgacgcc gtactctttg tcaacgacta ctattttggc caacgggaag
     1261 gcaatgcaag gagtttttga atattacaaa tcagtaacgt ttgtcagtaa ttgcggttct
     1321 cacccctcaa caactagcaa aggcagcccc ataaacacac agtatgtttt taaggacaat
     1381 agctcgacga ttgaaggtag atacccatac gacgttccag actacgctct gcaggctagt
     1441 ggtggaggag gctctggtgg aggcggtagc ggaggcggag ggtcggctag ccatatgcac
     1501 gatgaactat aatgaagcat gcttaattaa ggcgccttat ctcgaggggg gcggatccga
     1561 acaaaagctt atttctgaag aggacttgta atagagatct gataacaaca gtgtagatgt
     1621 aacaaaatcg actttgttcc cactgtactt ttagctcgta caaaatacaa tatacttttc
     1681 attt
//
