LOCUS       sequence-314855-        1700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast display plasmid for expression of C-terminal fusions with 
            Aga2p yeast agglutinin. The reporter DnbALFA was fused to the 
            N-terminus of Aga2p..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Zahradnik J, Dey D, Marciano S, Schreiber G
  TITLE     An enhanced yeast display platform demonstrates the binding 
            plasticity under various selection pressures
  JOURNAL   bioRxiv Preprint 2020
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            Preprint 2020"
COMMENT     Sequence Label: pJYDC3
FEATURES             Location/Qualifiers
     source          1..1700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        178..619
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    178..295
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     590..613
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             1401..1412
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1413..1439
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1570..1599
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cagcaacgcg gcactttgta actgagctgt cattttgctg gcccagctgg tcgaccgcgg
       61 ctaagcagta ctggtaccgt tgtggaaatg taaagagccc cattatctta gcctaaaaaa
      121 accttctctt tggaactttc agtaatacgc ttaactgctc attgctatat tgaagtacgg
      181 attagaagcc gccgagcggg tgacagccct ccgaaggaag actctcctcc gtgcgtcctc
      241 gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa
      301 taaagattct acaatactag cttttatggt tatgaagagg aaaaattggc agtaacctgg
      361 ccccacaaac cttcaaatga acgaatcaaa ttaacaacca taggatgata atgcgattag
      421 ttttttagcc ttatttctgg ggtaattaat cagcgaagcg atgatttttg atctattaac
      481 agatatataa atgcaaaaac tgcataacca ctttaactaa tactttcaac attttcggtt
      541 tgtattactt cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta atatacctct
      601 atactttaac gtcaaggaga aaaaaccccg gatcgaattc cctacttcat acattttcaa
      661 ttaagatgag gttcccatct attttcaccg ctgttgtttt tgctgcttct tctgctttgg
      721 ctgctccagc taatggtatg agcggtgaag ttcagctgca agaaagcggt ggtggtctgg
      781 ttcagcctaa tggtagcctg cgtctgagct gtaacgcaag cggtgttacc attagcgcac
      841 tgaatgcaat ggcaatgggt tggtatcgtc aggcaccggg tgaacgtcgt gttatggttg
      901 cagcagttag cgaacgtggt aatgcaatgt atcgtgaaag cgttaaaggt cgttttacca
      961 tttctcgtga taatgctaac aatatggtta gcctgcagat ggataatctg aaaccggaag
     1021 ataccgcagt gtattattgt catgttctgg aagatcgtgg tgatactttt catgattatt
     1081 ggggtcaggg cacccaggtt accgttagca gctctggtgg aggtggttcg ggcggaggtg
     1141 gtaacggatc taatggttct ggcggtagct ctgcacagga actgacaact atatgcgagc
     1201 aaatcccctc accaacttta gaatcgacgc cgtactcttt gtcaacgact actattttgg
     1261 ccaacgggaa ggcaatgcaa ggagtttttg aatattacaa atcagtaacg tttgtcagta
     1321 attgcggttc tcacccctca acaactagca aaggcagccc cataaacaca cagtatgttt
     1381 ttaaggacaa tagctcgacg attgaaggta gatacccata cgacgttcca gactacgctc
     1441 tgcaggctag tggtggagga ggctctggtg gaggcggtag cggaggcgga gggtcggcta
     1501 gccatatgca cgatgaacta taatgaagca tgcttaatta aggcgcctta tctcgagggg
     1561 ggcggatccg aacaaaagct tatttctgaa gaggacttgt aatagagatc tgataacaac
     1621 agtgtagatg taacaaaatc gactttgttc ccactgtact tttagctcgt acaaaataca
     1681 atatactttt catttctccg
//
