LOCUS       sequence-314863-        7522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Gwack Y, Sharma S, Nardone J, Tanasa B, Iuga A, Srikanth S, Okamura 
            H, Bolton D, Feske S, Hogan PG, Rao A
  TITLE     A genome-wide Drosophila RNAi screen identifies DYRK-family kinases 
            as regulators of NFAT.
  JOURNAL   Nature. 2006 Jun 1. 441(7093):646-50.
  PUBMED    16511445
REFERENCE   2  (bases 1 to 7522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Jun 1. 441(7093):646-50."
COMMENT     Sequence Label: pDEST12.2 DYRK2 WT
FEATURES             Location/Qualifiers
     source          1..7522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     634..650
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     634..650
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        673..691
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     673..690
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    716..740
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             785..808
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(2642..2666)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2697..2716)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2698..2716)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2727..2744)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2727..2743)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2736..2758)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    3148..3282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3185..3204)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3239..3258
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3408..3863
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3495..3514)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3705..3726
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3980..4337
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3980..4171
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4003..4023)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4188..4323
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4388..5182
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4442..4461)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5052..5071
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5246..5294
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(5335..5354)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5454..5476
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5514..5532)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5600..5704
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5705..5773,5774..6565)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5923..5942)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6736..7324
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7225..7244
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7478..7495
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      601 agcctaggcc gcgggacgga taacaatttc acacaggaaa cagctatgac cattaggcct
      661 ttgcaaaaag ctatttaggt gacactatag aaggtacgcc tgcaggtacc ggatcacaag
      721 tttgtacaaa aaagcaggct tcgaaggaga tagaaccaat tctctaagga aatacttaac
      781 catggactac aaagacgatg acgacaagct tgcggccgcg atgttaacca ggaaaccttc
      841 ggccgccgct cccgccgcct acccgaccgg ccgaggtggg gacagcgccg ttcgtcagct
      901 tcaggcttcc ccggggctcg gtgcaggggc cacccggagc ggagtgggga ctggcccgcc
      961 ctcccccatc gccctgccgc ctctccgggc cagcaacgct gccgccgcag cccacacgat
     1021 tggcggcagt aagcacacaa tgaatgatca cctgcatgtc ggcagccacg ctcacggaca
     1081 gatccaggtt caacagttgt ttgaggataa cagtaacaag cggacagtgc tcacgacaca
     1141 accaaatggg cttacaacag tgggcaaaac gggcttgcca gtggtgccag agcggcagct
     1201 ggacagcatt catagacggc aggggagctc cacctctcta aagtccatgg aaggcatggg
     1261 gaaggtgaaa gccaccccca tgacacctga acaagcaatg aagcaataca tgcaaaaact
     1321 cacagccttc gaacaccatg agattttcag ctaccctgaa atatatttct tgggtctaaa
     1381 tgctaagaag cgccagggca tgacaggtgg gcccaacaat ggtggctatg atgatgacca
     1441 gggatcatat gtgcaggtgc cccacgatca cgtggcttac aggtatgagg tcctcaaggt
     1501 cattgggaag gggagctttg ggcaggtggt caaggcctac gatcacaaag tccaccagca
     1561 cgtggcccta aagatggtgc ggaatgagaa gcgcttccac cggcaagcag cggaggagat
     1621 ccgaatcctg gaacacctgc ggaagcagga caaggataac acaatgaatg tcatccatat
     1681 gctggagaat ttcaccttcc gcaaccacat ctgcatgacg tttgagctgc tgagcatgaa
     1741 cctctatgag ctcatcaaga agaataaatt ccagggcttc agtctgcctt tggttcgcaa
     1801 gtttgcccac tcgattctgc agtgcttgga tgctttgcac aaaaacagaa taattcactg
     1861 tgaccttaag cccgagaaca ttttgttaaa gcagcagggt agaagcggta ttaaagtaat
     1921 tgattttggc tccagttgtt acgagcatca gcgtgtctac acgtacatcc agtcgcgttt
     1981 ttaccgggct ccagaagtga tccttggggc caggtatggc atgcccattg atatgtggag
     2041 cctgggctgc attttagcag agctcctgac gggttacccc ctcttgcctg gggaagatga
     2101 aggggaccag ctggcctgta tgattgaact gttgggcatg ccctcacaga aactgctgga
     2161 tgcatccaaa cgagccaaaa attttgtgag ctccaagggt tatccccgtt actgcactgt
     2221 cacggctctc tcagatggct ctgtggtcct aaacggaggc cgttcccgga gggggaaact
     2281 gaggggccca ccggagagca gagagtgggg gaacgcgctg aaggggtgtg atgatcccct
     2341 tttccttgac ttcttaaaac agtgtttaga gtgggatcct gcagtgcgca tgaccccagg
     2401 ccaggctttg cggcacccct ggctgaggag gcggttgcca aagcctccca ccggggagaa
     2461 aacgtcagtg aaaaggataa ctgagagcac cggtgctatc acatctatat ccaagttacc
     2521 tccaccttct agctcagctt ccaaactgag gactaatttg gcgcagatga cagatgccaa
     2581 tgggaatatt cagcagagga cagtgttgcc aaaacttgtt agctgacctc gagatatcta
     2641 gacccagctt tcttgtacaa agtggtgatc gcgtgcatgc gacgtcatag ctctctccct
     2701 atagtgagtc gtattataag ctaggcactg gccgtcgttt tacaacgtcg tgactgggaa
     2761 aactgctagc ttgggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     2821 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     2881 gttaaactag ctgcatatgc ttgctgcttg agagttttgc ttactgagta tgatttatga
     2941 aaatattata cacaggagct agtgattcta attgtttgtg tattttagat tcacagtccc
     3001 aaggctcatt tcaggcccct cagtcctcac agtctgttca tgatcataat cagccatacc
     3061 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     3121 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     3181 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     3241 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcgatcctgc attaatgaat
     3301 cggccaacgc gcggggagag gcggtttgcg tattggctgg cgtaatagcg aagaggcccg
     3361 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     3421 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3481 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3541 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3601 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     3661 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3721 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     3781 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     3841 caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
     3901 atttcacacc gcatacgcgg atctgcgcag caccatggcc tgaaataacc tctgaaagag
     3961 gaacttggtt aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     4021 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4081 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4141 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4201 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4261 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4321 gcctaggctt ttgcaaaaag cttgattctt ctgacacaac agtctcgaac ttaaggctag
     4381 agccaccatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4441 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     4501 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4561 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4621 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4681 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4741 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4801 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4861 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     4921 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4981 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5041 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5101 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5161 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     5221 cccaacctgc catcacgatg gccgcaataa aatatcttta ttttcattac atctgtgtgt
     5281 tggttttttg tgtgaatcga tagcgataag gatccgcgta tggtgcactc tcagtacaat
     5341 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     5401 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     5461 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     5521 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5581 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5641 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5701 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5761 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5821 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5881 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5941 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6001 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6061 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6121 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6181 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6241 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6301 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6361 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6421 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6481 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6541 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6601 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6661 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6721 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6781 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6841 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6901 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6961 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7021 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7081 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7141 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7201 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7261 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7321 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7381 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7441 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7501 ggaagagctc gcgaatgcat gt
//
