LOCUS       sequence-314872-        7921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-expression of mouse CD5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Matson CA, Choi S, Livak F, Zhao B, Mitra A, Love PE, Singh NJ
  TITLE     CD5 dynamically calibrates basal NF-kappaB signaling in T cells 
            during thymic development and peripheral activation.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Jun 23;117(25):14342-14353. doi: 
            10.1073/pnas.1922525117. Epub 2020 Jun 8.
  PUBMED    32513716
REFERENCE   2  (bases 1 to 7921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1922525117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-06-23- 23"; volume: "117"; issue: "25"; pages: 
            "14342-14353"
COMMENT     Sequence Label: MIGR1-CD5
FEATURES             Location/Qualifiers
     source          1..7921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     58..80
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     96..112
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1637..2204
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1654..2206
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1798..1815)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2025..2044
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2218..2220,2221..2223,2224..2937)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2263..2284)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2524..2543)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2871..2892
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             3039..3553
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    3715..3731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3739..3745,3746..3763,3764..3769))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3784..3805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3922..3939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4093..4681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4173..4192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4852..5643,5644..5712))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5475..5494
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5713..5817)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5885..5903
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5941..5963)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6063..6082
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6276..6298
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6426..6445
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             6645..7161
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    7225..7566
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             join(7633..7921,1..128)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
ORIGIN
        1 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
       61 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
      121 taggcgccgg aattagatcc cattatccat ggactcccac gaagtgctgc tggctgccac
      181 gtacctgctg ggaacgctgg cggctttctg cctcggacag tctggaaggg gtggcctaga
      241 tatccaggtg atgctaagtg gctccaactc caagtgtcag ggtcaagtgg agatccagat
      301 ggaaaacaag tggaaaacag tgtgcagttc cagttggagg ctgagccagg accattcgaa
      361 aaatgcccag caggcctctg cagtgtgcaa agagctgaga tgtggtgacc ccttggccct
      421 tggtcctttc ccttcattga acagacccca gaaccaggtc ttctgccaag gatctccgtg
      481 gtctatatcc aactgcaaca acacaagttc acaagaccag tgccttccgc tgagcctgat
      541 ctgcctagag ccccagagaa cgacacctcc acccacaacc accccaccca ccaccgtgcc
      601 agagcccaca gctcctccca gattgcagct ggtgccagga cacgaaggcc tgaggtgcac
      661 aggtgtggtg gaattctaca atggcagctg gggtggcacc atcctctaca aggccaagga
      721 caggcccctg ggcctgggga acctcatctg taagtctctg cagtgtggct ctttcttgac
      781 acatctgtcc gggacagagg cagcagggac accagctcct gcagagctga gggaccccag
      841 gcccttgcca attcgatggg aggccccgaa cgggagctgt gtctcactac agcagtgctt
      901 ccagaaaaca acggcccagg agggcggcca ggcgctcacc gtcatctgct ctgatttcca
      961 gcccaaggtt cagagccgcc tggtcggggg cagcagtgtg tgtgagggca tcgctgaagt
     1021 gcgccagaga tcacagtggg aggccctgtg tgacagttct gcagccaggg gccggggacg
     1081 gtgggaagag ctatgtcggg agcagcagtg tggcgacctc atctccttcc acacggtgga
     1141 tgctgacaaa acctcccccg ggttcctctg tgcccaggag aagctgtctc agtgttacca
     1201 tctccagaaa aaaaaacact gcaacaagag ggtattcgtc acatgccaag acccaaaccc
     1261 agcaggcctg gccccaggca ctgtggcaag catcatcctg acccttgtac tcctggtggt
     1321 gctgctggcc atgtgtggtc ctctggtcta caagaagctg gtgaagaaat ttcgtcagaa
     1381 aaagcagcgt cagtggattg gccccacagg agtgaaccag aacatgtctt tccatcgaag
     1441 ccacacagca actgtgcggt cccaagttga gaacccaaca gcctctcatg tggacaatga
     1501 atacagccag cctcccagga actctcacct gtctgcttat ccagctctgg aaggagccct
     1561 acaccgatct tctacacaac ctgacaactc ctctgacagt gactatgacc tgcaagtggc
     1621 tcagagactg taaaattccg cccccccccc ctaacgttac tggccgaagc cgcttggaat
     1681 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     1741 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     1801 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     1861 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     1921 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     1981 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     2041 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     2101 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     2161 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg
     2221 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2281 gacgtaaacg gccacaagtt cagcgtgtct ggcgagggcg agggcgatgc cacctacggc
     2341 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2401 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     2461 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2521 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2581 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2641 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     2701 atcaaggcga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     2761 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2821 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2881 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatga
     2941 attaattaag aattatcaag cttatcgata ccgtcgacct gcagccaagc ttatcgataa
     3001 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     3061 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat
     3121 agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata
     3181 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     3241 tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     3301 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     3361 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct
     3421 ccgatagact gcgtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     3481 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     3541 gggggtcttt catgggtaac agtttcttga agttggagaa caacattctg agggtaggag
     3601 tcgaatatta agtaatcctg actcaattag ccactgtttt gaatccacat actccaatac
     3661 tcctgaaata gttcattatg gacagcgcag aaagagctgg ggagaattgt gaaattgtta
     3721 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     3781 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3841 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3901 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3961 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     4021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     4141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4501 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4801 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4861 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4921 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4981 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     5041 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     5101 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     5161 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     5221 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     5281 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     5341 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     5401 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     5461 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     5521 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     5581 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5641 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5701 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5761 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5821 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     5881 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     5941 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     6001 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     6061 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     6121 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     6181 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     6241 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     6301 ggcgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
     6361 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     6421 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
     6481 gccacgatgc gtccggcgta gaggcgatta gtccaatttg ttaaagacag gatatcagtg
     6541 gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca
     6601 tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     6661 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aatacataac
     6721 tgagaataga gaagttcaga tcaaggttag gaacagagag acagcagaat atgggccaaa
     6781 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
     6841 gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
     6901 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
     6961 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggcgcgc
     7021 cagtcctccg atagactgcg tcgcccgggt acccgtattc ccaataaagc ctcttgctgt
     7081 ttgcatccga atcgtggact cgctgatcct tgggagggtc tcctcagatt gattgactgc
     7141 ccacctcggg ggtctttcat ttggaggttc caccgagatt tggagacccc tgcccaggga
     7201 ccaccgaccc ccccgccggg aggtaagctg gccagcggtc gtttcgtgtc tgtctctgtc
     7261 tttgtgcgtg tttgtgccgg catctaatgt ttgcgcctgc gtctgtacta gttagctaac
     7321 tagctctgta tctggcggac ccgtggtgga actgacgagt tctgaacacc cggccgcaac
     7381 cctgggagac gtcccaggga ctttgggggc cgtttttgtg gcccgacctg aggaagggag
     7441 tcgatgtgga atccgacccc gtcaggatat gtggttctgg taggagacga gaacctaaaa
     7501 cagttcccgc ctccgtctga atttttgctt tcggtttgga accgaagccg cgcgtcttgt
     7561 ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg
     7621 tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa
     7681 gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc
     7741 ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac
     7801 cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca
     7861 gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc
     7921 a
//
