LOCUS       sequence-314887-        4677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-DYRK1B
FEATURES             Location/Qualifiers
     source          1..4677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1988..2087)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2104..2123)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2105..2123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2128..2144)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2128..2144)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2575..3366
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3459..4047
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3948..3967
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4201..4218
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4377..4404)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4496..4582)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4631..4653
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4645..4662
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4646..4662
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggccgtccc accgggccat
      121 ggtcccttct ctggcttccc agggccccag gagcacacgc aggtattgcc tgatgtgcgg
      181 ctactgcctc ggaggctgcc cctggccttc cgggatgcaa cctcagcccc gctgcgtaag
      241 ctctctgtgg acctcatcaa gacctacaag cacatcaatg aggtatacta tgcgaagaag
      301 aagcggcggg cccagcaggc gccaccccag gattcgagca acaagaagga gaagaaggtc
      361 ctgaaccatg gttatgatga cgacaaccat gactacatcg tgcgcagtgg cgagcgctgg
      421 ctggagcgct acgaaattga ctcgctcatt ggcaaaggct cctttggcca ggtggtgaaa
      481 gcctatgatc atcagaccca ggagcttgtg gccatcaaga tcatcaagaa caaaaaggct
      541 ttcctgaacc aggcccagat tgagctgcgg ctgctggagc tgatgaacca gcatgacacg
      601 gagatgaagt actatatagt acacctgaag cggcacttca tgttccggaa ccacctgtgc
      661 ctggtatttg agctgctgtc ctacaacctg tacgacctcc tgcgcaacac ccacttccgc
      721 ggcgtctcgc tgaacctgac ccggaagctg gcgcagcagc tctgcacggc actgctcttt
      781 ctggccacgc ctgagctcag catcattcac tgcgacctca agcccgaaaa catcttgctg
      841 tgcaacccca agcgcagcgc catcaagatt gtggacttcg gcagctcctg ccagcttggc
      901 cagaggatct accagtatat ccagagccgc ttctaccgct cacctgaggt gctcctgggc
      961 acaccctacg acctggccat tgacatgtgg tccctgggct gcatccttgt ggagatgcac
     1021 accggagagc ccctcttcag tggctccaat gaggtcgacc agatgaaccg cattgtggag
     1081 gtgctgggca tcccaccggc cgccatgctg gaccaggcgc ccaaggctcg caagtacttt
     1141 gaacggctgc ctgggggtgg ctggacccta cgaaggacga aagaactcag gaaggattac
     1201 cagggccccg ggacacggcg gctgcaggag gtgctgggcg tgcagacggg cgggcccggg
     1261 ggccggcggg cgggggagcc gggccacagc cccgccgact acctccgctt ccaggacctg
     1321 gtgctgcgca tgctggagta tgagcccgcc gcccgcatca gccccctggg ggctctgcag
     1381 cacggcttct tccgccgcac ggccgacgag gccaccaaca cgggcccggc aggcagcagt
     1441 gcctccacct cgcccgcgcc cctcgacacc tgcccctctt ccagcaccgc cagctccatc
     1501 tccagttctg gaggctccag tggctcctcc agtgacaacc ggacctaccg ctacagcaac
     1561 cgatattgtg ggggccctgg gccccctatc acagactgtg agatgaacag cccccaggtc
     1621 ccaccctccc agccgctgcg gccctgggca gggggtgatg tgccccacaa gacacatcaa
     1681 gcccctgcct ctgcctcgtc actgcctggg accggggccc agttaccccc ccagccccga
     1741 taccttggtc gtcccccatc accaacctca ccaccacccc cggagctgat ggatgtgagc
     1801 ctggtgggcg gccctgctga ctgctcccca cctcacccag cgcctgcccc ccagcacccg
     1861 gctgcctcag ccctccggac tcggatgact ggaggtcgtc cacccctccc gcctcctgat
     1921 gaccctgcca ctctggggcc tcacctgggc ctccgtggtg taccccagag cacagcagcc
     1981 agctcgtgcc caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     2041 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     2101 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     2161 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag
     2221 accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca
     2281 ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg
     2341 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     2401 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
     2461 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     2521 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg
     2581 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     2641 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     2701 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     2761 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     2821 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     2881 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     2941 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     3001 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     3061 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     3121 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     3181 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     3241 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     3301 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     3361 aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     3421 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3481 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3541 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3601 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3661 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3721 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3781 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3841 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3901 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3961 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4021 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4081 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4141 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4201 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     4261 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     4321 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
     4381 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
     4441 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
     4501 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
     4561 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
     4621 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccc
//
