LOCUS       sequence-31489-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HRMT1L3
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             179..196
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct
      181 ggttccgcgt ggtagtgacc tccaggagga tgaggatggt gtttatttca gctcatacgg
      241 gcattatggg atacatgaag aaatgctaaa ggacaaaata cgaacagaaa gctaccgaga
      301 tttcatatac caaaatccac atatcttcaa agacaaggta gttttggatg ttgggtgtgg
      361 aactggaatt ctctctatgt ttgctgctaa agctggggcg aagaaggttc ttggagttga
      421 tcaatctgaa atactttacc aggcaatgga tattataaga ctaaataaac ttgaagatac
      481 tattacacta attaaaggaa agattgaaga agttcatctt cctgtagaaa aagtagatgt
      541 tatcatatct gagtggatgg gctattttct tctgtttgag tctatgttag attctgtcct
      601 ttatgcaaag aacaaatact tggcaaaagg aggctcggtc taccctgaca tttgcactat
      661 cagccttgta gcagtgagtg atgtgaataa acatgctgat agaattgctt tttgggatga
      721 tgtctatggc ttcaagatgt cctgcatgaa gaaagcagtt attccagaag ctgttgtgga
      781 agttttagat ccgaagactc ttatttcaga accttgtggt attaagcata tagattgcca
      841 tacgacgtct atctc
//
