LOCUS       sequence-314890-       10672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid provides the HomeR#1 gene drive element harboring a rescue, 
            gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted 
            at Pol-gamma-35 site #1 via HDR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Kandul NP, Liu J, Bennett JB, Marshall JM, Akbari OS
  TITLE     A confinable home and rescue gene drive for population modification.
  JOURNAL   Elife. 2021 Mar 5;10. pii: 65939. doi: 10.7554/eLife.65939.
  PUBMED    33666174
REFERENCE   2  (bases 1 to 10672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-03-5"; volume: "10. pii: 65939. doi"; pages: " 
            10.7554/eLife.65939"
COMMENT     Sequence Label: 
            1073A(HomeR1)=pBac-[Pol-gamma-35.HL-decPol-gamma-35-p10]-[U6-gRNA#1(
            Pol-gamma-35)]-[3xp3-GFP-SV40]-Pol-gamma-35.HR-[Opie2-dsRed-SV40]
FEATURES             Location/Qualifiers
     source          1..10672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1463..1883
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        1904..1979
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(2269..2271,2272..2985)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2269..2271,2272..2982)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2311..2332)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2572..2591)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2919..2940
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3107..3228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             join(4480..4482,4483..4485,4486..5160)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(4663..4683)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     4881..4900
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5075..5098
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    5274..5395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   6063..6097
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6278..6294)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6278..6294)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6291..6313)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6302..6318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6326..6332,6333..6350,6351..6356))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6371..6392
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6509..6526)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6680..7268)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6760..6779)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7439..8230,8231..8299))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8062..8081
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8300..8404)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8472..8490
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8528..8550)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8650..8669
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8863..8885
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8877..8894
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8878..8894
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   9598..9660
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ccacggactt cctagtggaa ccacatcgcg cactggagca cttctacaac atgcagcgtg
       61 agagcaagat ctggtggatg cgtctttcct cgaatcccag tcgctaccgc atcgtacctt
      121 gcgatttggc tgaggatctc aatcccaacg actatcaagc catcgatatc cgcacgagct
      181 acggtgatgc aggagaagtg accgtggagc aactgagcct ggtgagaata gtggacgaca
      241 aagacttccg gctgcccgat gcgcgaacgg gtgaaattgt gcaaccgact gtcataaggt
      301 ctgtgataga gctggaaact accacctgcg gtaagtggtc atcataacta tctttgaaaa
      361 tctttctata ttattatcct ttagctctgc ttctggatgg ctgtgatcat ggccgggact
      421 cgcaatccct gctcttacac cgcgttctgg cgccatatca atgtggaatt gcctgtgtgg
      481 aaagtgattc cgagctatct gctgacttgt ctgatctttg tcagcatctg aaacatgttc
      541 tcaaccacgc cggtcttaga ctttctgaag gggatggaat tcgtacgacg aagaacgctt
      601 ctcatttagc agagcacttg ctggaaacgg atatgctcgg cataccttat acacttgtta
      661 taaacgagca aacgctaaga aacggactga tgcagttgcg cagccgggac acacgcctcg
      721 ccgaaacgat ccatattagt gacgtgcccg attacttgtt gaacatcttc aagaattagt
      781 ttaaactgaa tcgtttttaa aataacaaat caattgtttt ataatattcg tacgattctt
      841 tgattatgta ataaaatgtg atcattagga agattacgaa aaatataaaa aatatgagtt
      901 ctgtgtgtat aacaaatgct gtaaacgcca caattgtgtt tgttgcaaat aaacccatga
      961 ttatttgatt aaaattgttg ttttctttgt tcatagacaa tagtgtgttt tgcctaaacg
     1021 tgtactgcat aaactccatg cgagtgtata gcgagctagt ggctaacgct tgccccacca
     1081 aagtagattc gtcaaaatcc tcaatttcat caccctcctc caagtttaac atttggccgt
     1141 cggaattaac ttctaaagat gccacataat ctaataaatg aaatagagat tcaaacgtgg
     1201 cgtcatcgtc cgtttcgacc atttccgaaa agaactcggg cataaactct atgatttctc
     1261 tggacgtggt gttgtcgaaa ctctcaaagt acgcagtcag gaacgtgcgc gacatgtcgt
     1321 cgggaaactc gcgcggaaac atgttgttgt aaccgaacgg gtcccatagc gccaaaacca
     1381 aatctgccag cgtcaataga atgagcacga tgccgacaat ggagctggct tggatagcga
     1441 ttcgagttaa tacgtagaat tcttttttgc tcacctgtga ttgctcctac tcaaatacaa
     1501 aaacatcaaa ttttctgtca ataaagcata tttatttata tttattttac aggaaagaat
     1561 tccttttaaa gtgtatttta acctataatg aaaaacgatt aaaaaaaata cataaaataa
     1621 ttcgaaaatt tttgaatagc ccaggttgat aaaaattcat ttcatacgtt ttataactta
     1681 tgcccctaag tattttttga ccatagtgtt tcaattctac attaatttta cagagtagaa
     1741 tgaaacgcca cctactcagc caagaggcga aaaggttagc tcgccaagca gagagggcgc
     1801 cagtgctcac tactttttat aattctcaac ttctttttcc agactcagtt cgtatatata
     1861 gacctatttt caatttaacg tcggcgcagc cgggacacaa ggcgttttag agctagaaat
     1921 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     1981 tttttttttg ctagcaattc gagctcgccc ggggatctaa ttcaattaga gactaattca
     2041 attagagcta attcaattag gatccaagct tatcgatttc gaaccctcga ccgccggagt
     2101 ataaatagag gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac
     2161 gtcgctaagc gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatcgggg
     2221 taccgctaga gtcgacggta cgatccaccg gtcgccacct cgagcaaaat gagcaagggc
     2281 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     2341 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     2401 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     2461 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     2521 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     2581 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     2641 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     2701 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     2761 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     2821 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     2881 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2941 accgccgccg ggatcactct cggcatggac gagctgtaca agtaagcggc cgcgactcta
     3001 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     3061 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     3121 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3181 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaaa gcttttaatt
     3241 aaggctggcg gagaccatac acataagcga tgtaccggac tatttattaa atatatttaa
     3301 aaactaaaaa aaacaaccag gggctacata aagtcgcaga gatattggcg gacattaaag
     3361 cccaccacct tgccaatctg caggacaaac tccaagccaa tggtgcactg cagcctagcg
     3421 gaaccttcca ttatgttttg ttcgccggag acgaagctta gtagattgag gtgcaccaaa
     3481 gttgagatct gggagagaag gttgcaggtc agaccgactt tctcctctag aatggcgtag
     3541 aagatggcca gcaaacgatc gatgctaaag gatttgggtc ccaaagtggt ggacattttc
     3601 tccgtggtct aaaaggtatt aaaataagca aatggagtca tcgaaaaatt gaataaccca
     3661 ccttggctct ggcgttgacc gtctgcatcc gctttcgctg ttttccatgg tgcttgacga
     3721 acaatcgctt gtcctgcttg gcggcattgt ggctggccaa aaaggcagcg atcaacagaa
     3781 atttggcata gtagggcagc tccagcgact gcgccagctt gcgaacgctc tgatcttcta
     3841 tggcggtgaa atcttcaact tcttccgcgg gtttttcaat tcgcatataa atctgggtta
     3901 aggcggaacg cagtggtcca gctatatgcc ggcgcgccca tactcggtgg cctccccacc
     3961 accaactttt ttgcactgca aaaaaacacg cttttgcacg cgggcccata catagtacaa
     4021 actctacgtt tcgtagacta ttttacataa atagtctaca ccgttgtata cgctccaaat
     4081 acactaccac acattgaacc tttttgcagt gcaaaaaagt acgtgtcggc agtcacgtag
     4141 gccggcctta tcgggtcgcg tcctgtcacg tacgaatcac attatcggac cggacgagtg
     4201 ttgtcttatc gtgacaggac gccagcttcc tgtgttgcta accgcagccg gacgcaactc
     4261 cttatcggaa caggacgcgc ctccatatca gccgcgcgtt atctcatgcg cgtgaccgga
     4321 cacgaggcgc ccgtcccgct tatcgcgcct ataaatacag cccgcaacga tctggtaaac
     4381 acagttgaac agcatctgtt acagcgacac aacatgagcc ggtccaacaa cgccaacgcg
     4441 cccacgccat ccaaccgccg ccgcaacctg tctctggtga tggtgcgctc ctccaagaac
     4501 gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg gcaccgtgaa cggccacgag
     4561 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gccacaacac cgtgaagctg
     4621 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttccag
     4681 tacggctcca aggtgtacgt gaagcacccc gccgacatcc ccgactacaa gaagctgtcc
     4741 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     4801 gtgacccagg actcctccct gcaggacggc tgcttcatct acaaggtgaa gttcatcggc
     4861 gtgaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     4921 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacaa ggccctgaag
     4981 ctgaaggacg gcggccacta cctggtggag ttcaagtcca tctacatggc caagaagccc
     5041 gtgcagctgc ccggctacta ctacgtggac tccaagctgg acatcacctc ccacaacgag
     5101 gactacacca tcgtggagca gtacgagcgc accgagggcc gccaccacct gttcctgtag
     5161 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     5221 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     5281 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5341 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagct
     5401 tctaggccgg ccgatctcgg atctgacaat gttcagtgca gagactcggc tacgcctcgt
     5461 ggactttgaa gttgaccaac aatgtttatt cttacctcta atagtcctct gtggcaaggt
     5521 caagattctg ttagaagcca atgaagaacc tggttgttca ataacatttt gttcgtctaa
     5581 tatttcacta ccgcttgacg ttggctgcac ttcatgtacc tcatctataa acgcttcttc
     5641 tgtatcgctc tggacgtcat cttcacttac gtgatctgat atttcactgt cagaatcctc
     5701 accaacaagc tcgtcatcgc tttgcagaag agcagagagg atatgctcat cgtctaaaga
     5761 actacccatt ttattatata ttagtcacga tatctataac aagaaaatat atatataata
     5821 agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa
     5881 aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat
     5941 cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat
     6001 gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa
     6061 tgcatgcgtc aattttacgc agactatctt tctagggtta aaaaagattt gcgctttact
     6121 cgacctaaac tttaaacacg tcatagaatc ttcgtttgac aaaaaccaca ttgtggccaa
     6181 gctgtgtgac gcgacgcgcg ctaaagaatg gcaaaccaag tcgcgcgagc gtcgactcta
     6241 gaggatcccc gggtaccgag ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa
     6301 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6361 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6421 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6481 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6541 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6601 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6661 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6721 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6781 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6841 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6901 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6961 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7021 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7081 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7141 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7201 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7261 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7321 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7381 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7441 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7501 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7561 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7621 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7681 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7741 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7801 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7861 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7921 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7981 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8041 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8101 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8161 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8221 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8281 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8341 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8401 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     8461 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     8521 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     8581 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     8641 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     8701 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
     8761 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     8821 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     8881 aaacgacggc cagtgccaag ctttgtttaa aaatataaca aaattgtgat cccacaaaat
     8941 gaagtggggc aaaatcaaat aattaatagt gtccgtaaac ttgttggtct tcaacttttt
     9001 gaggaacacg ttggacggca aatccgtgac tataacacaa gttgatttaa taattttagc
     9061 caacacgtcg ggctgcgtgt tttttgccga cgcgtctgtg tacacgttga ttaactggtc
     9121 gattaaactg ttgaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
     9181 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
     9241 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
     9301 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
     9361 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
     9421 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
     9481 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
     9541 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
     9601 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     9661 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     9721 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     9781 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
     9841 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
     9901 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
     9961 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
    10021 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
    10081 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
    10141 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
    10201 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
    10261 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
    10321 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
    10381 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
    10441 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
    10501 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
    10561 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
    10621 tttgatcgca cggttcccac aatggtaatt cgagctcgcc cggggtccta gg
//
