LOCUS       sequence-314891-       10686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid provides the HomeR#2 gene-drive element harboring a rescue, 
            gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted 
            at Pol-gamma-35 site #2 via HDR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Kandul NP, Liu J, Bennett JB, Marshall JM, Akbari OS
  TITLE     A confinable home and rescue gene drive for population modification.
  JOURNAL   Elife. 2021 Mar 5;10. pii: 65939. doi: 10.7554/eLife.65939.
  PUBMED    33666174
REFERENCE   2  (bases 1 to 10686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-03-5"; volume: "10. pii: 65939. doi"; pages: " 
            10.7554/eLife.65939"
COMMENT     Sequence Label: 1073B(HomeR2)
            =pBac-[Pol-gamma-35.HL-decPol-gamma-35-p10...
FEATURES             Location/Qualifiers
     source          1..10686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1419..1481
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2185..2202)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2185..2201)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2194..2216)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2410..2429)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2529..2551
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2589..2607)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2675..2779
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2780..2848,2849..3640)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2998..3017)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3811..4399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4300..4319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4553..4570
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4687..4708
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4723..4728,4729..4746,4747..4753)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4761..4777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4766..4788
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4785..4801
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4785..4801
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   4982..5016
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    5684..5805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(5919..6593,6594..6596,6597..6599))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(5981..6004)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(6179..6198)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6396..6416
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     polyA_signal    7864..7985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(8107..8820,8821..8823))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(8110..8820,8821..8823))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8152..8173)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8501..8520
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8760..8781
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_RNA        complement(9113..9188)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(9209..9629)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
ORIGIN
        1 cacagccatc cagaagcaga gctaaaggat aataatatag aaagattttc aaagatagtt
       61 atgatgacca cttaccgcag gtggtagttt ccagctctat cacagacctt atgacagtcg
      121 gttgcacaat ttcacccgtt cgcgcatcgg gcagccggaa gtctttgtcg tccactattc
      181 tcaccaggct cagttgctcc acggtcactt ctcctgcatc accgtagctc gtgcggatat
      241 cgatggcttg atagtcgttg ggattgagat cctcagccaa atcgcaaggt acgatgcggt
      301 agcgactggg attcgaggaa agacgcatcc accagatctt gctctcacgc tgcatgttgt
      361 agaagtgctc cagtgcgcga tgtggttcca ctaggaagtc cgtggcctag gaccccgggc
      421 gagctcgaat taaccattgt gggaaccgtg cgatcaaaca aacgcgagat accggaagta
      481 ctgaaaaaca gtcgctccag gccagtggga acatcgatgt tttgttttga cggacccctt
      541 actctcgtct catataaacc gaagccagct aagatggtat acttattatc atcttgtgat
      601 gaggatgctt ctatcaacga aagtaccggt aaaccgcaaa tggttatgta ttataatcaa
      661 actaaaggcg gagtggacac gctagaccaa atgtgttctg tgatgacctg cagtaggaag
      721 acgaataggt ggcctatggc attattgtac ggaatgataa acattgcctg cataaattct
      781 tttattatat acagccataa tgtcagtagc aagggagaaa aggttcaaag tcgcaaaaaa
      841 tttatgagaa acctttacat gagcctgacg tcatcgttta tgcgtaagcg tttagaagct
      901 cctactttga agagatattt gcgcgataat atctctaata ttttgccaaa tgaagtgcct
      961 ggtacatcag atgacagtac tgaagagcca gtaatgaaaa aacgtactta ctgtacttac
     1021 tgcccctcta aaataaggcg aaaggcaaat gcatcgtgca aaaaatgcaa aaaagttatt
     1081 tgtcgagagc ataatattga tatgtgccaa agttgtttct gactgactaa taagtataat
     1141 ttgtttctat tatgtataag ttaagctaat tacttatttt ataatacaac atgactgttt
     1201 ttaaagtaca aaataagttt atttttgtaa aagagagaat gtttaaaagt tttgttactt
     1261 tatagaagaa attttgagtt tttgtttttt tttaataaat aaataaacat aaataaattg
     1321 tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat atctattcaa
     1381 attaataaat aaacctcgat atacagaccg ataaaacaca tgcgtcaatt ttacgcatga
     1441 ttatctttaa cgtacgtcac aatatgatta tctttctagg gttaaataat agtttctaat
     1501 ttttttatta ttcagcctgc tgtcgtgaat accgtatatc tcaacgctgt ctgtgagatt
     1561 gtcgtattct agccttttta gtttttcgct catcgacttg atattgtccg acacattttc
     1621 gtcgatttgc gttttgatca aagacttgag cagagacacg ttaatcaact gttcaaattg
     1681 atccatatta acgatatcaa cccgatgcgt atatggtgcg taaaatatat tttttaaccc
     1741 tcttatactt tgcactctgc gttaatacgc gttcgtgtac agacgtaatc atgttttctt
     1801 ttttggataa aactcctact gagtttgacc tcatattaga ccctcacaag ttgcaaaacg
     1861 tggcattttt taccaatgaa gaatttaaag ttattttaaa aaatttcatc acagatttaa
     1921 agaagaacca aaaattaaat tatttcaaca gtttaatcga ccagttaatc aacgtgtaca
     1981 cagacgcgtc ggcaaaaaac acgcagcccg acgtgttggc taaaattatt aaatcaactt
     2041 gtgttatagt cacggatttg ccgtccaacg tgttcctcaa aaagttgaag accaacaagt
     2101 ttacggacac tattaattat ttgattttgc cccacttcat tttgtgggat cacaattttg
     2161 ttatattttt aaacaaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
     2221 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     2281 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     2341 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     2401 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     2461 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     2521 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     2581 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     2641 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2701 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     2761 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     2821 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     2881 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     2941 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     3001 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     3061 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     3121 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     3181 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     3241 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     3301 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     3361 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     3421 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     3481 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     3541 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     3601 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     3661 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     3721 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3781 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3841 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3901 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3961 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4021 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4081 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4141 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4201 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4261 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4321 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4381 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4441 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     4501 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     4561 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     4621 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     4681 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     4741 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     4801 catgattacg aattcgagct cggtacccgg ggatcctcta gagtcgacgc tcgcgcgact
     4861 tggtttgcca ttctttagcg cgcgtcgcgt cacacagctt ggccacaatg tggtttttgt
     4921 caaacgaaga ttctatgacg tgtttaaagt ttaggtcgag taaagcgcaa atctttttta
     4981 accctagaaa gatagtctgc gtaaaattga cgcatgcatt cttgaaatat tgctctctct
     5041 ttctaaatag cgcgaatccg tcgctgtgca tttaggacat ctcagtcgcc gcttggagct
     5101 cccgtgaggc gtgcttgtca atgcggtaag tgtcactgat tttgaactat aacgaccgcg
     5161 tgagtcaaaa tgacgcatga ttatctttta cgtgactttt aagatttaac tcatacgata
     5221 attatattgt tatttcatgt tctacttacg tgataactta ttatatatat attttcttgt
     5281 tatagatatc gtgactaata tataataaaa tgggtagttc tttagacgat gagcatatcc
     5341 tctctgctct tctgcaaagc gatgacgagc ttgttggtga ggattctgac agtgaaatat
     5401 cagatcacgt aagtgaagat gacgtccaga gcgatacaga agaagcgttt atagatgagg
     5461 tacatgaagt gcagccaacg tcaagcggta gtgaaatatt agacgaacaa aatgttattg
     5521 aacaaccagg ttcttcattg gcttctaaca gaatcttgac cttgccacag aggactatta
     5581 gaggtaagaa taaacattgt tggtcaactt caaagtccac gaggcgtagc cgagtctctg
     5641 cactgaacat tgtcagatcc gagatcggcc ggcctagaag ctttaagata cattgatgag
     5701 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     5761 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     5821 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     5881 ctctacaaat gtggtatggc tgattatgat ctagagtcct acaggaacag gtggtggcgg
     5941 ccctcggtgc gctcgtactg ctccacgatg gtgtagtcct cgttgtggga ggtgatgtcc
     6001 agcttggagt ccacgtagta gtagccgggc agctgcacgg gcttcttggc catgtagatg
     6061 gacttgaact ccaccaggta gtggccgccg tccttcagct tcagggcctt gtggatctcg
     6121 cccttcagca cgccgtcgcg ggggtacagg cgctcggtgg aggcctccca gcccatggtc
     6181 ttcttctgca ttacggggcc gtcggagggg aagttcacgc cgatgaactt caccttgtag
     6241 atgaagcagc cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg
     6301 aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttctt gtagtcgggg
     6361 atgtcggcgg ggtgcttcac gtacaccttg gagccgtact ggaactgggg ggacaggatg
     6421 tcccaggcga agggcagggg gccgcccttg gtcaccttca gcttcacggt gttgtggccc
     6481 tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggtgccc
     6541 tccatgcgca ccttgaagcg catgaactcc ttgatgacgt tcttggagga gcgcaccatc
     6601 accagagaca ggttgcggcg gcggttggat ggcgtgggcg cgttggcgtt gttggaccgg
     6661 ctcatgttgt gtcgctgtaa cagatgctgt tcaactgtgt ttaccagatc gttgcgggct
     6721 gtatttatag gcgcgataag cgggacgggc gcctcgtgtc cggtcacgcg catgagataa
     6781 cgcgcggctg atatggaggc gcgtcctgtt ccgataagga gttgcgtccg gctgcggtta
     6841 gcaacacagg aagctggcgt cctgtcacga taagacaaca ctcgtccggt ccgataatgt
     6901 gattcgtacg tgacaggacg cgacccgata aggccggcct acgtgactgc cgacacgtac
     6961 ttttttgcac tgcaaaaagg ttcaatgtgt ggtagtgtat ttggagcgta tacaacggtg
     7021 tagactattt atgtaaaata gtctacgaaa cgtagagttt gtactatgta tgggcccgcg
     7081 tgcaaaagcg tgtttttttg cagtgcaaaa aagttggtgg tggggaggcc accgagtatg
     7141 ggcgcgccgg catatagctg gaccactgcg ttccgcctta acccagattt atatgcgaat
     7201 tgaaaaaccc gcggaagaag ttgaagattt caccgccata gaagatcaga gcgttcgcaa
     7261 gctggcgcag tcgctggagc tgccctacta tgccaaattt ctgctgatcg ctgccttttt
     7321 ggccagccac aatgccgcca agcaggacaa gcgattgttc gtcaagcacc atggaaaaca
     7381 gcgaaagcgg atgcagacgg tcaacgccag agccaaggtg ggttattcaa tttttcgatg
     7441 actccatttg cttattttaa taccttttag accacggaga aaatgtccac cactttggga
     7501 cccaaatcct ttagcatcga tcgtttgctg gccattttct acgccattct agaggagaaa
     7561 gtcggtctga cctgcaacct tctctcccag atctcaactt tggtgcacct caatctacta
     7621 agcttcgtct ccggcgaaca aaacataatg gaaggttccg ctaggctgca gtgcaccatt
     7681 ggcttggagt ttgtcctgca gattggcaag gtggtgggct ttaatgtccg ccaatatctc
     7741 tgcgacttta tgtagcccct ggttgttttt tttagttttt aaatatattt aataaatagt
     7801 ccggtacatc gcttatgtgt atggtctccg ccagccttgt gtcccggctt taattaaaag
     7861 ctttaagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     7921 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     7981 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     8041 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ctagagtcgc
     8101 ggccgcttac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
     8161 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct
     8221 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
     8281 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt
     8341 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc
     8401 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
     8461 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
     8521 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
     8581 gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt
     8641 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag
     8701 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac
     8761 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
     8821 cattttgctc gaggtggcga ccggtggatc gtaccgtcga ctctagcggt accccgattg
     8881 tttagcttgt tcagctgcgc ttgtttattt gcttagcttt cgcttagcga cgtgttcact
     8941 ttgcttgttt gaattgaatt gtcgctccgt agacgaagcg cctctattta tactccggcg
     9001 gtcgagggtt cgaaatcgat aagcttggat cctaattgaa ttagctctaa ttgaattagt
     9061 ctctaattga attagatccc cgggcgagct cgaattgcta gcaaaaaaaa aagcaccgac
     9121 tcggtgccac tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc
     9181 tctaaaacgc tgcgcaactg catcagtccg acgttaaatt gaaaataggt ctatatatac
     9241 gaactgagtc tggaaaaaga agttgagaat tataaaaagt agtgagcact ggcgccctct
     9301 ctgcttggcg agctaacctt ttcgcctctt ggctgagtag gtggcgtttc attctactct
     9361 gtaaaattaa tgtagaattg aaacactatg gtcaaaaaat acttaggggc ataagttata
     9421 aaacgtatga aatgaatttt tatcaacctg ggctattcaa aaattttcga attattttat
     9481 gtattttttt taatcgtttt tcattatagg ttaaaataca ctttaaaagg aattctttcc
     9541 tgtaaaataa atataaataa atatgcttta ttgacagaaa atttgatgtt tttgtatttg
     9601 agtaggagca atcacaggtg agcaaaaaag aattctacgt attaactcga atcgctatcc
     9661 aagccagctc cattgtcggc atcgtgctca ttctattgac gctggcagat ttggttttgg
     9721 cgctatggga cccgttcggt tacaacaaca tgtttccgcg cgagtttccc gacgacatgt
     9781 cgcgcacgtt cctgactgcg tactttgaga gtttcgacaa caccacgtcc agagaaatca
     9841 tagagtttat gcccgagttc ttttcggaaa tggtcgaaac ggacgatgac gccacgtttg
     9901 aatctctatt tcatttatta gattatgtgg catctttaga agttaattcc gacggccaaa
     9961 tgttaaactt ggaggagggt gatgaaattg aggattttga cgaatctact ttggtggggc
    10021 aagcgttagc cactagctcg ctatacactc gcatggagtt tatgcagtac acgtttaggc
    10081 aaaacacact attgtctatg aacaaagaaa acaacaattt taatcaaata atcatgggtt
    10141 tatttgcaac aaacacaatt gtggcgttta cagcatttgt tatacacaca gaactcatat
    10201 tttttatatt tttcgtaatc ttcctaatga tcacatttta ttacataatc aaagaatcgt
    10261 acgaatatta taaaacaatt gatttgttat tttaaaaacg attcagttta aactaattct
    10321 tgaagatgtt caacaagtaa tcgggcacgt cactaatatg gatcgtttcg gcgaggcgtg
    10381 tgtcgcggga ccgcaactgc atcagtccgt ttcttagcgt ttgctcgttt ataacaagtg
    10441 tataaggtat gccgagcata tccgtttcca gcaagtgctc tgctaaatga gaagcgttct
    10501 ttgtcgtacg aattccatcc ccttcagaaa gtctaagacc ggcgtggttg agaacatgtt
    10561 tcagatgctg acaaagatca gacaagtcag cagatagctc ggaatcactt tccacacagg
    10621 caattccaca ttgatatggc gccagaacgc ggtgtaagag cagggattgc gagtcccggc
    10681 catgat
//
