LOCUS       sequence-314892-        2314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Agrobacterium terminator for plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: lvl0 NOSt
FEATURES             Location/Qualifiers
     source          1..2314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      6..63
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(87..104)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(258..846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(338..357)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1029..1123)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1144..1803)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1718..1737
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1965..1983
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1987..2030)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      2050..2302
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa
       61 agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      121 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      181 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      241 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga
      301 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      361 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      421 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      481 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      541 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      601 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      661 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
      721 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      781 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      841 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
      901 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      961 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg
     1021 aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     1081 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
     1141 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
     1201 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
     1261 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
     1321 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
     1381 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
     1441 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
     1501 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     1561 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
     1621 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     1681 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     1741 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     1801 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     1861 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac
     1921 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     1981 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgg
     2041 tctctgcttg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     2101 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     2161 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     2221 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     2281 gtgtcatcta tgttactaga tccgcttgag accc
//
