LOCUS       sequence-314893-        2270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: lvl0 LEC2t
FEATURES             Location/Qualifiers
     source          1..2270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      32..89
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(113..130)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(284..872)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(364..383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1055..1149)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1170..1829)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1744..1763
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1991..2009
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2013..2056)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 tacacgcgat aaaacgcttg agaccctgca gtccggcaaa aaagggcaag gtgtcaccac
       61 cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca
      121 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      181 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      241 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc
      301 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      361 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      421 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      481 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      541 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      601 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      661 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      721 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
      781 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
      841 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
      901 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
      961 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1021 atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc
     1081 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     1141 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac
     1201 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     1261 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     1321 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     1381 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     1441 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     1501 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     1561 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
     1621 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     1681 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     1741 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     1801 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     1861 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     1921 acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt
     1981 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
     2041 cgctaaggat gatttctgga attcggtctc tgcttcgttc atagctaact tgtaagaatt
     2101 gtcgaaaact tttgaatttg ttaaaaaaaa aaatgacaat taaagtgttt ataatatgtt
     2161 actagtgtga aattatattg tatatactgt atcaattttt atttttgttt aaaaaaatca
     2221 tcttgcttct atttagaaat taaagataac ttgggaacag tgccttgcct
//
