LOCUS       sequence-314895-        2778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yellow fluorescent protein coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: lvl0 mVenus CDS
FEATURES             Location/Qualifiers
     source          1..2778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      13..70
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(94..111)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(265..853)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(345..364)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1036..1130)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1151..1810)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1725..1744
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1972..1990
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1994..2037)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             join(2054..2056,2057..2059,2060..2773)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2099..2120)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2360..2379)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2707..2728
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gagaccctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa
       61 accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt
      121 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      181 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      241 taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa
      301 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      361 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      421 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      481 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      541 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      601 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      661 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
      721 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
      781 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
      841 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
      901 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
      961 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1021 cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     1081 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     1141 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     1201 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     1261 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     1321 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     1381 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     1441 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     1501 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     1561 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg
     1621 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     1681 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     1741 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     1801 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     1861 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga
     1921 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     1981 atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg
     2041 aattcggtct ctaatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2101 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     2161 cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca agctgcccgt
     2221 gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg cccgctaccc
     2281 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2341 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     2401 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2461 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcaccgccga
     2521 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcgg
     2581 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2641 gcccgacaac cactacctga gctaccagtc caagctgagc aaagacccca acgagaagcg
     2701 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     2761 gctgtacaag taagcttt
//
