LOCUS       sequence-314896-        2778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Far red fluorescent protein with SV40 NLS, codon optimised for 
            Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: lvl0 mKate2 CDS
FEATURES             Location/Qualifiers
     source          1..2778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      17..74
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(98..115)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(269..857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(349..368)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1040..1134)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1155..1814)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1729..1748
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1976..1994
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1998..2041)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             2754..2774
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctttgagacc ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt
       61 taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct
      121 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      181 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      241 accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc
      301 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      361 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      421 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      481 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      541 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      601 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      661 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      721 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      781 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      841 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
      901 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
      961 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1021 ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     1081 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     1141 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     1201 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     1261 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     1321 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     1381 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1441 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1501 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1561 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     1621 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1681 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1741 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     1801 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     1861 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac
     1921 tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     1981 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
     2041 ctggaattcg gtctctaatg gtgtcagaac taataaaaga aaacatgcac atgaagcttt
     2101 atatggaggg aaccgtcaac aaccatcatt tcaaatgcac atctgagggc gaaggtaagc
     2161 cgtacgaggg aacacagacg atgcgtatta aggctgttga aggtggtcct ttgcctttcg
     2221 catttgatat tctcgctact agttttatgt acggtagcaa aacattcata aaccacaccc
     2281 aggggatacc tgacttcttc aagcagagct tcccggaagg tttcacatgg gaacgagtga
     2341 cgacatacga ggatggcggt gttcttacgg caacccagga tactagcctc caagatgggt
     2401 gcctaatcta taatgttaag atccgaggag tgaatttccc aagcaatggc ccagtcatgc
     2461 agaagaagac tctcggatgg gaggcttcta cggaaacttt gtaccctgcg gatggaggtt
     2521 tagagggacg agccgacatg gcattaaagc ttgtaggggg aggccatctc atctgcaatc
     2581 ttaagactac gtatagatca aaaaagcccg caaagaatct caaaatgcca ggtgtctact
     2641 atgtagatag aagactggaa agaattaaag aggccgataa agaaacatac gttgagcaac
     2701 acgaagtagc cgtcgcccga tattgcgatc taccttccaa gctgggtcac cgaccgaaga
     2761 aaaagagaaa agtttgag
//
