LOCUS       sequence-314910-        2314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Agrobacterium terminator for plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: lvl0 MASt
FEATURES             Location/Qualifiers
     source          1..2314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      11..68
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(92..109)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(263..851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(343..362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1034..1128)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1149..1808)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1723..1742
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1970..1988
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1992..2035)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      2055..2307
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
ORIGIN
        1 gaccctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac
       61 cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg
      121 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      181 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      241 aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa
      301 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      361 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      421 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      481 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      541 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      601 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      661 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
      721 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      781 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
      841 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
      901 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
      961 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1021 gctcgaggct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
     1081 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
     1141 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     1201 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     1261 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     1321 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     1381 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     1441 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     1501 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     1561 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
     1621 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     1681 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     1741 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     1801 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     1861 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt
     1921 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     1981 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa
     2041 ttcggtctct gcttcttgga ctcccatgtt ggcaaaggca accaaacaaa caatgaatga
     2101 tccgctcctg catatggggc ggtttgagta tttcaactgc catttgggct gaattgtaga
     2161 catgctcctg tcagaaattc cgtgatctta ctcaatattc agtaatctcg gccaatatcc
     2221 taaatgtgcg tggctttatc tgtctttgta ttgtttcatc aattcatgta acgtttgctt
     2281 ttcttatgaa ttttcaaata aattatccgc ttga
//
