LOCUS       sequence-314931-        4284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dex inducible system trans activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: lvl0 LhGR
FEATURES             Location/Qualifiers
     source          1..4284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      18..75
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(99..116)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(270..858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(350..369)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1041..1135)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1156..1815)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1730..1749
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1977..1995
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1999..2042)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             3940..4284
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
ORIGIN
        1 gctttgagac cctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct
       61 ttaaaaccga aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc
      121 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      181 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      241 aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat
      301 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      361 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      421 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
      481 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
      541 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
      601 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
      661 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
      721 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
      781 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
      841 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
      901 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
      961 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1021 tctgacagct cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     1081 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     1141 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     1201 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     1261 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     1321 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     1381 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     1441 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     1501 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     1561 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
     1621 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     1681 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     1741 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     1801 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     1861 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa
     1921 ctcgagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     1981 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
     2041 tctggaattc ggtctctaat ggctagtgaa gctcgaaaaa caaagaaaaa aatcaaaggg
     2101 attcagcaag ccactgcagg agtctcacaa gacacttcgg aaaatcctaa caaaacaata
     2161 gttcctgcag cattaccaca gctcacccct accttggtgt cactgctgga ggtgattgaa
     2221 cccgaggtgt tgtatgcagg atatgatagc tctgttccag attcagcatg gagaattatg
     2281 accacactca acatgttagg tgggcgtcaa gtgattgcag cagtgaaatg ggcaaaggcg
     2341 ataccaggct tcagaaactt acacctggat gaccaaatga ccctgctaca gtactcatgg
     2401 atgtttctca tggcatttgc cctgggttgg agatcataca gacaatcaag tggaaacctg
     2461 ctctgctttg ctcctgatct gattattaat gagcagagaa tgtctctacc ctgcatgtat
     2521 gaccaatgta aacacatgct gtttgtctcc tctgaattac aaagattgca ggtatcctat
     2581 gaagagtatc tctgtatgaa aaccttactg cttctctcct cagttcctaa ggaaggtctg
     2641 aagagccaag agttatttga tgagattcga atgacttata tcaaagagct aggaaaagcc
     2701 atcgtcaaaa gggaagggaa ctccagtcag aactggcaac ggttttacca actgacaaag
     2761 cttctggact ccatgcatga ggtggttgag aatctcctta cctactgctt ccagacattt
     2821 ttggataaga ccatgagtat tgaattccca gagatgttag ctgaaatcat cactaatcag
     2881 ataccaaaat attcaaatgg aaatatcaaa aagcttctgt ttcatcaaaa atctactagc
     2941 aaaccggtaa cgttatacga cgtcgctgaa tacgccggcg tttctcatca aaccgtttct
     3001 agagtggtta accaggcttc acatgttagc gctaaaaccc gggaaaaagt tgaagctgcc
     3061 atggctgagc tcaactacat cccgaaccgt gttgcgcagc agctggctgg taaacaaagc
     3121 ttgctgatcg gtgtcgcgac ctcgagcttg gccctgcacg cgccgtcgca aattgtcgcg
     3181 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
     3241 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
     3301 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact
     3361 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc
     3421 tcccatgagg acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
     3481 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
     3541 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
     3601 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
     3661 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
     3721 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgagga cagctcatgt
     3781 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     3841 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtg
     3901 tcactggtga aaagaaaaac cactagtgga tcggaattcg ccaattttaa tcaaagtggg
     3961 aatattgctg atagctcatt gtccttcact ttcactaaca gtagcaacgg tccgaacctc
     4021 ataacaactc aaacaaattc tcaagcgctt tcacaaccaa ttgcctcctc taacgttcat
     4081 gataacttca tgaataatga aatcacggct agtaaaattg atgatggtaa taattcaaaa
     4141 ccactgtcac ctggttggac ggaccaaact gcgtataacg cgtttggaat cactacaggg
     4201 atgtttaata ccactacaat ggatgatgta tataactatc tattcgatga tgaagatacc
     4261 ccaccaaacc caaaaaaaga gtaa
//
