LOCUS       sequence-314934-        3710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: lvl0 fluc CDS
FEATURES             Location/Qualifiers
     source          1..3710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      14..71
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(95..112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(266..854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(346..365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1037..1131)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1152..1811)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1726..1745
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1973..1991
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1995..2038)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             2055..3707
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2121..2139)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tgagaccctg cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa
       61 aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg
      121 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      181 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      241 gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca
      301 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      361 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      421 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      481 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      541 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      601 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      661 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      721 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      781 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      841 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      901 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      961 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1021 acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga
     1081 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
     1141 tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
     1201 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
     1261 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
     1321 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg
     1381 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     1441 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     1501 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     1561 atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg
     1621 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     1681 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
     1741 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     1801 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     1861 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactcg
     1921 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     1981 tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg
     2041 gaattcggtc tctaatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc
     2101 cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg
     2161 ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt
     2221 acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc
     2281 acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt
     2341 tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca
     2401 acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa
     2461 ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa
     2521 cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt
     2581 ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca
     2641 tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct
     2701 gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg
     2761 cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt
     2821 tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga
     2881 gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg
     2941 ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg
     3001 gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta
     3061 tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca cccgaggggg
     3121 atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc
     3181 tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta
     3241 tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg
     3301 gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg
     3361 accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat
     3421 ccatcttgct ccaacacccc aacatcttcg acgctggtgt cgcaggtctt cccgacgatg
     3481 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa
     3541 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag
     3601 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
     3661 gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgtagctt
//
