LOCUS       sequence-31494-e         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LOC201475
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..49
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             59..76
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             625..642
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct
       61 ggttccgcgt ggtagtccgc gtcccgcana tttcaagctg caagtgatta tcattggttc
      121 acgtggtgtt ggtaaaacct ctctgatgga acgttttacg gatgatactt tctgtgaagc
      181 ttgtaaaagt acagtaggtg ttgattttaa aatcaaaact gtagaactgc gtggtaaaaa
      241 aattcgtctg caaatctggg atacagcang tcaagaacgt ttcaattcan ttacttctgc
      301 ttattaccgt agtgcaaaag gcatcattct cgtctatgac ataaccaaga aggagacgtt
      361 tgacgactta ccaaagtgga tgaaaatgat agacaagtac gcgagcgagg acgccgagtt
      421 gcttctcgtg ggcaacaaat tagattgcga gaccgatcgc gagattacgc gccagcaggg
      481 cgagaaattc gcgcagcaga tcaccggcat gcgcttttgc gaagcctcng cgaaggacaa
      541 tttcancgtc gatgagatat ttttgaaact tgtggacgat atcctcaaaa agatgtgatg
      601 acgaagcttg cggccgcact cgagcaccac caccaccacc actga
//
