LOCUS       sequence-314942-        2361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: lvl0 APT1p
FEATURES             Location/Qualifiers
     source          1..2361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      63..120
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(144..161)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(315..903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(395..414)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1086..1180)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1201..1860)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1775..1794
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2022..2040
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2044..2087)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 cgcctcttct cctccgccgg tagaccccac caccttctat ttcccaatgt gagaccctgc
       61 agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga
      121 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      181 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      241 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      301 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc
      361 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      421 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      481 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      541 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      601 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      661 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      721 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
      781 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
      841 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
      901 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
      961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg
     1081 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
     1141 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
     1201 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     1261 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     1321 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     1381 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     1441 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     1501 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     1561 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     1621 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
     1681 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     1741 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     1801 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     1861 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     1921 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
     1981 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     2041 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcggtct
     2101 ctggagggat gagttatagg tttggttaag acttgaaaag tcattgatca cggcttttgt
     2161 ccattattcc gtgtttaaag taatttacat tctcttttaa gatctctcag tccaaagaca
     2221 atgcaaacaa taataatttc tccacttgtt tctcatcgtc tctgtcttgc tcgtgctgtt
     2281 ccttgcaacc gtcttctcaa caaccaccac cgtgctcctc cttcgatccg cctctcaaac
     2341 caccgttcaa ccacctcact c
//
