LOCUS       sequence-31497-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CHKA
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     274..293
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    396..420
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             435..457
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             476..493
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             503..520
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 cctgattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg
       61 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag
      121 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
      181 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
      241 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
      301 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
      361 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
      421 cctctagaaa taattttgtt taactttaag aaggagatat accatgggca gcagccatca
      481 tcatcatcat cacagcagcg gcctggttcc gcgtggtagt ccgcaaccgc cagccgatga
      541 acagccggaa ccgcgtaccc gccgtcgtgc gtacctgtgg tgcaaagaat tcctgccggg
      601 tgcttggcgc ggtctgcgtg aagatgaatt tcatatttcc gttattcgtg gcggcctgag
      661 caacatgctg tttcagtgta gcctgccgga tactaccgca acgctgggcg atgaaccacg
      721 taaagtgctg ctgcgcctgt atggcgctat tctgcaaatg cgctcgtgta acaaagaggg
      781 ttcggaacag gcacagaaag aaaacgagtt ccagggtgcg gaagctatgg ttctggagag
      841 cgtgatgttc gcaatcctgg cagacgcagc ctgggtccga aactgtacgg catttttcca
      901 cagggtcgcc tggaacagtt catcccat
//
