LOCUS       sequence-314987-       12647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Flp-mediated cassette exchange. Expresses tdTomato in the 
            presence of (r)tTA, switching to the cell cycle suppressor p21 in 
            the presence of Cre and (r)tTA activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12647)
  AUTHORS   Ahmadzadeh E, Bayin NS, Qu X, Singh A, Madisen L, Stephen D, Zeng H,
            Joyner AL, Rosello-Diez A
  TITLE     A collection of genetic mouse lines and related tools for inducible 
            and reversible intersectional mis-expression.
  JOURNAL   Development. 2020 May 28;147(10). pii: dev.186650. doi: 
            10.1242/dev.186650.
  PUBMED    32366677
REFERENCE   2  (bases 1 to 12647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2020-05-28"; volume: "147(10). pii: 
            dev.186650. doi"; pages: " 10.1242/dev.186650"
COMMENT     Sequence Label: pDragon-p21
FEATURES             Location/Qualifiers
     source          1..12647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    414..447
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     promoter        3009..3323
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    3017..3035
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3053..3071
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3088..3106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3124..3142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3160..3178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3195..3213
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3231..3249
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3296..3320
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(3333..3366)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3376..3385
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3515..3533)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3783..3802
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3977..4000
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    4813..4861
                     /note="synthetic polyadenylation signal"
     polyA_signal    4870..5346
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5069..5088)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     protein_bind    complement(5350..5383)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5895..6483
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5948..5968)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6366..6377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6508..6525)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6514..6721
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    9219..9252
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        9279..9778
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9314..9337)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9711..9730
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(10426..10442)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10426..10442)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10439..10461)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10450..10466
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10474..10480,10481..10498,10499..10504))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10519..10540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10657..10674)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10828..11416)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10908..10927)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11587..12378,12379..12447))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12210..12229
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12448..12552)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12620..12638
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgaagttc
      421 ctattcttca aatagtatag gaacttcttt cggccggcca atcgattgga tccgttttat
      481 gccgcggtta ccgtgaagat atcttaatta agaattcgag ctcgctagag ccccatcctc
      541 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt
      601 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc
      661 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac
      721 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag
      781 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc
      841 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc
      901 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt
      961 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat
     1021 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa
     1081 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc
     1141 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc
     1201 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat
     1261 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg
     1321 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc
     1381 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc
     1441 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa
     1501 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     1561 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     1621 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     1681 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagcg
     1741 agctcggtac ccagagcccc atcctcactg actccgtcct ggagttggat gagagataat
     1801 ggccttacgt tgtgccaggg gagggtcggg ctggatttag caagatttac cttctccaaa
     1861 gagcggtgct gcagtggcac agctgcccac ggaggtgggg gggtcaccgt ccctggaggt
     1921 gatgaagaac tgtggggatg tggcactgag ggacatggcc agtgggcacg gtgggtgggt
     1981 tggggttggt cttggggatc ttggagggct tttccagcct tcatgatttg acgattgtat
     2041 gaacatctac atggcaattc tccagctgcc tgtcccagtc ctactgaccc agctgtatct
     2101 ctccaggcaa gctcttccac cccttctgct tgcatccaga caccatcaaa catgcaggct
     2161 cagacacagg gaccagcagt gtctgtggcc tttttgtgct cctctccatg ctgggtttta
     2221 acttgctctt tgtccttcta tcctatcttc ttatccttaa ggctgttctg aacgctgtga
     2281 cttggagagt gtcccagagc cctcaacacc tgcatgtccc acgtccatgc tgtcctgcac
     2341 ttccttatcc ccaagatctg cctctccgtg atgcactgaa ttggcaaaca tgtgtcaccc
     2401 cagaccaaca atgtcacagc aaactccccc ttgataggac aagggggaat ggctttacac
     2461 tgagacaggg gaggtttggg ttggatatga ggaggcagtt tttcccccag agggtggtga
     2521 cgcactgaac aggttgccca aggaggctgt ggatgcccca tccctgcagg cattcaaggc
     2581 caggctggat gtggctctgg gcagcctggg ctgctggttg atgaccctgc acatagcagg
     2641 gggttggatc tggatgagca ctgtgctcct ttgcaaccca ggccgttcta tgattctgtc
     2701 attctaaatc tctctttcag cctaaagctt tttccccgta tccccccagg tgtctgcagg
     2761 ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc
     2821 gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag
     2881 ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc
     2941 tttggggggg ggctgtccct ctaggggtac ccggggatcc accggtatcg attacctatc
     3001 acaaattcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     3061 agtgatagag aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
     3121 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     3181 gtatgtcgag tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
     3241 tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc
     3301 gtttagtgaa ccgtcagatc gcctatttaa atataacttc gtatagcata cattatacga
     3361 agttattcgc gagccgccac catggtgagc aagggcgagg aggtcatcaa agagttcatg
     3421 cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag
     3481 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     3541 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac
     3601 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag
     3661 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc
     3721 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac
     3781 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
     3841 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac
     3901 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac
     3961 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     4021 cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggtac cggcagcacc
     4081 ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt gattaaggag
     4141 tttatgaggt ttaaagtcag gatggagggc agcatgaacg ggcacgagtt tgagattgaa
     4201 ggagaggggg aagggaggcc ctatgagggc acccagacag caaagctgaa agtgacaaag
     4261 ggcggccctc tgccattcgc atgggacatc ctgtctcctc agtttatgta tggaagtaaa
     4321 gcctacgtga aacaccctgc tgacattcca gattacaaga aactgagctt cccagagggg
     4381 tttaagtggg aaagagtgat gaacttcgaa gacgggggcc tggtgactgt cacccaggac
     4441 agctctctgc aggacggcac tctgatctat aaggtgaaaa tgcgcggcac caattttccc
     4501 cctgatggcc cagtcatgca gaagaagaca atgggctggg aggcttcaac agaaaggctg
     4561 taccccagag acggcgtgct gaagggagag atccaccagg cactgaagct gaaagatgga
     4621 gggcattatc tggtcgaatt caaaaccatc tacatggcca agaaacccgt gcagctgcct
     4681 ggctactatt acgtcgacac aaagctggat attactagcc ataacgaaga ctacaccatt
     4741 gtggagcagt atgagcggag cgaggggagg caccatctgt ttctgtacgg catggacgag
     4801 ctgtacaagt aaaataaaat atctttattt tcattccatc tgtgtgttgg ttttttgtgt
     4861 gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     4921 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     4981 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     5041 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     5101 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcaggct
     5161 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     5221 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     5281 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     5341 gtccttctga taacttcgta tagcatacat tatacgaagt tatcacgcgt gccaccatgt
     5401 ccaatcctgg tgatgtccga cctgttccgc acaggagcaa agtgtgccgt tgtctcttcg
     5461 gtcccgtgga cagtgagcag ttgcgccgtg attgcgatgc gctcatggcg ggctgtctcc
     5521 aggaggcccg agaacggtgg aactttgact tcgtcacgga gacgccgctg gagggcaact
     5581 ttgtctggga gcgcgttcgg agcctagggc tgcccaaggt ctacctgagc cctgggtccc
     5641 gcagccgtga cgacctggga ggggacaaga ggcccagtac ttcctctgcc ctgctgcagg
     5701 ggccagctcc agaggaccac gtggccttgt cgctgtcttg cactctggtg tctgagcggc
     5761 ctgaagattc cccgggtggg cccggaacat ctcagggccg aaaacggagg cagaccagcc
     5821 tgacagattt ctatcactcc aagcgcagat tggtcttctg caagagaaaa ccctgatacg
     5881 taaagcttgt cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5941 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6001 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6061 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6121 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6181 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6241 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     6301 ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6361 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6421 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6481 cgcatcgaca ccgagcgctg ctcgaggcct cgactgtgcc ttctagttgc cagccatctg
     6541 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     6601 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     6661 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     6721 acacgtggga tcctctagag ccccatcctc actgactccg tcctggagtt ggatgagaga
     6781 taatggcctt acgttgtgcc aggggagggt cgggctggat ttagcaagat ttaccttctc
     6841 caaagagcgg tgctgcagtg gcacagctgc ccacggaggt gggggggtca ccgtccctgg
     6901 aggtgatgaa gaactgtggg gatgtggcac tgagggacat ggccagtggg cacggtgggt
     6961 gggttggggt tggtcttggg gatcttggag ggcttttcca gccttcatga tttgacgatt
     7021 gtatgaacat ctacatggca attctccagc tgcctgtccc agtcctactg acccagctgt
     7081 atctctccag gcaagctctt ccaccccttc tgcttgcatc cagacaccat caaacatgca
     7141 ggctcagaca cagggaccag cagtgtctgt ggcctttttg tgctcctctc catgctgggt
     7201 tttaacttgc tctttgtcct tctatcctat cttcttatcc ttaaggctgt tctgaacgct
     7261 gtgacttgga gagtgtccca gagccctcaa cacctgcatg tcccacgtcc atgctgtcct
     7321 gcacttcctt atccccaaga tctgcctctc cgtgatgcac tgaattggca aacatgtgtc
     7381 accccagacc aacaatgtca cagcaaactc ccccttgata ggacaagggg gaatggcttt
     7441 acactgagac aggggaggtt tgggttggat atgaggaggc agtttttccc ccagagggtg
     7501 gtgacgcact gaacaggttg cccaaggagg ctgtggatgc cccatccctg caggcattca
     7561 aggccaggct ggatgtggct ctgggcagcc tgggctgctg gttgatgacc ctgcacatag
     7621 cagggggttg gatctggatg agcactgtgc tcctttgcaa cccaggccgt tctatgattc
     7681 tgtcattcta aatctctctt tcagcctaaa gctttttccc cgtatccccc caggtgtctg
     7741 caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt
     7801 gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc
     7861 ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg
     7921 gggctttggg ggggggctgt ccctctagag ccccatcctc actgactccg tcctggagtt
     7981 ggatgagaga taatggcctt acgttgtgcc aggggagggt cgggctggat ttagcaagat
     8041 ttaccttctc caaagagcgg tgctgcagtg gcacagctgc ccacggaggt gggggggtca
     8101 ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac tgagggacat ggccagtggg
     8161 cacggtgggt gggttggggt tggtcttggg gatcttggag ggcttttcca gccttcatga
     8221 tttgacgatt gtatgaacat ctacatggca attctccagc tgcctgtccc agtcctactg
     8281 acccagctgt atctctccag gcaagctctt ccaccccttc tgcttgcatc cagacaccat
     8341 caaacatgca ggctcagaca cagggaccag cagtgtctgt ggcctttttg tgctcctctc
     8401 catgctgggt tttaacttgc tctttgtcct tctatcctat cttcttatcc ttaaggctgt
     8461 tctgaacgct gtgacttgga gagtgtccca gagccctcaa cacctgcatg tcccacgtcc
     8521 atgctgtcct gcacttcctt atccccaaga tctgcctctc cgtgatgcac tgaattggca
     8581 aacatgtgtc accccagacc aacaatgtca cagcaaactc ccccttgata ggacaagggg
     8641 gaatggcttt acactgagac aggggaggtt tgggttggat atgaggaggc agtttttccc
     8701 ccagagggtg gtgacgcact gaacaggttg cccaaggagg ctgtggatgc cccatccctg
     8761 caggcattca aggccaggct ggatgtggct ctgggcagcc tgggctgctg gttgatgacc
     8821 ctgcacatag cagggggttg gatctggatg agcactgtgc tcctttgcaa cccaggccgt
     8881 tctatgattc tgtcattcta aatctctctt tcagcctaaa gctttttccc cgtatccccc
     8941 caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca
     9001 ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc
     9061 ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt
     9121 acatccctgg gggctttggg ggggggctgt ccctctagag tcgacggcgc gccgtaaaag
     9181 cttggcatag atcttattgc agaagccata tgttttaggt gccagggcgt gcccttgggc
     9241 tccccgggcg cgtttgggcg gccgcatcga attctaccgg gtaggggagg cgcttttccc
     9301 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     9361 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     9421 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     9481 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     9541 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     9601 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     9661 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     9721 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     9781 ctgcaatcgc cgctagccgc caccatgaaa aagcctgaac tcaccgcgac gtctgtcgag
     9841 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     9901 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     9961 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
    10021 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
    10081 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
    10141 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
    10201 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
    10261 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgta
    10321 agtgctagcc cctaggcctg aatcgaagtt cctattcttc aaaaggtata ggaacttcga
    10381 gctcgtcgac tgcagaggcc tgcatgcaag cttggcgtaa tcatggtcat agctgtttcc
    10441 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    10501 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    10561 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
    10621 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    10681 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    10741 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    10801 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    10861 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    10921 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    10981 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    11041 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    11101 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    11161 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    11221 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
    11281 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    11341 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    11401 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    11461 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    11521 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    11581 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    11641 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    11701 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    11761 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    11821 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    11881 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    11941 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    12001 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    12061 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    12121 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    12181 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    12241 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    12301 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    12361 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    12421 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    12481 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    12541 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
    12601 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
