LOCUS       sequence-314994-        1119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for use in CIT (chemically induced trimerization) system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Wu HD, Kikuchi M, Dagliyan O, Aragaki AK, Nakamura H, Dokholyan NV, 
            Umehara T, Inoue T
  TITLE     Rational design and implementation of a chemically inducible 
            heterotrimerization system.
  JOURNAL   Nat Methods. 2020 Sep;17(9):928-936. doi: 10.1038/s41592-020-0913-x.
            Epub 2020 Aug 3.
  PUBMED    32747768
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0913-x"; journalName: "Nat Methods"; date: 
            "2020-09"; volume: "17"; issue: "9"; pages: "928-936"
COMMENT     Sequence Label: FRB2N-CFP-CAAX
FEATURES             Location/Qualifiers
     source          1..1119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      319..328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(325..327,328..330,331..1041)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(370..391)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(631..650)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     978..999
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgatcctct ggcatgagat gtggcatgaa ggcctggaag aggcatctcg tttgtacttt
       61 ggggaaagga acgtgaaagg catgtttgag gtgctggagc ccttgcatgc tatgatggaa
      121 cggggccccc agactctgaa ggaaacatcc tttaatcagg cctatggtcg agatttaatg
      181 gaggcccaag agtggtgcag gaagtacatg aaatcagggc agtcgacgag tgctggtggt
      241 agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg ctggtggtcg ggtaccgcgg
      301 gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
      361 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      421 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      481 aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc
      541 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      601 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      661 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      721 gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat
      781 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
      841 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      901 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
      961 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1021 ggcatggacg agctgtacaa gtccggactc agatctaaga tgagcaaaga tggtaaaaag
     1081 aagaaaaaga agtcaaagac aaagtgtgta attatgtaa
//
