LOCUS       sequence-315-exp        5090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5090)
  TITLE     Fire Lab C. Elegans Vector Kit 2005
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD158.87
FEATURES             Location/Qualifiers
     source          1..5090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1176..1204)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1952..1972
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(2530..2552)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2652..2671
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2749..2767)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2835..2939
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2940..3008,3009..3800)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3158..3177)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3971..4559
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4460..4479
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4713..4730
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4847..4868
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4883..4888,4889..4906,4907..4913)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4921..4937
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4926..4948
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4945..4961
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4945..4961
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagagtgg agagtgtagt ccctcgtcgg ctctctgtgt atttctttca
       61 atgcctgtgt ctcggtctat ctttgtctct atctgtttta aaagtttttt gcaaagtttc
      121 aatttctttt ttattttctc ttcttttttt gcgcatttat tttttttaaa gagcgctgtt
      181 cttcaattgg aaaacagtat aacgatcgaa ataaaaaaaa aacttgaaat agtatcggaa
      241 ttgagatggg ggcaattgaa attgaagacg caacaaaaac atgtttttat cgtctgaaat
      301 tatctaaact ttgaatctaa cgtgtcaatg gctactttta cagtagactt tttagagtaa
      361 tgttaaaaag gataatatag gtttttcttc aaactatttt gtcacgccgc atatattctt
      421 ttcatattcc attcgcaaag tgcatgatgt aacaaaaatg aaacgtcata attttttaat
      481 ctttcacact ttttttgccg gccgttaaat caaaatcaaa tcaacttaat ccaacacgtg
      541 cgggcttgca attttattac accggtgtcc tacagcgcca tgttatcttc tttgattaga
      601 gatctctcac gcaacatttg acattgaaaa ttttgtctcc gaattgttgt gtacctactt
      661 tgtcccgatg tcccaaattt tcactgataa caagagatat ctcaactgtt cttatcacgt
      721 tgatacgtag aacgttttac actaaaccaa attgcagtcc ccttctacga accgtttgct
      781 aattgttttg catagaacag tgatcaatgt gttgtgctca ctagtcaatg atgttcgtct
      841 ctcatttttc ctacaacttc actgaaaagg ttttgaatat ctggtgattg taggcaatca
      901 caccacagtt aatggcctgg caaataatga taagcgtgtg ataaacctca caaaaatacg
      961 cctagaagtg gtgatatcgc tagttttgtg tcctttcgat ttttttcgtg ttgctttttt
     1021 ctgtggataa acctttgaac tttacatttt gttttcagaa taaccgctct agaggatcct
     1081 atcgattcgc ggccgctgta cacccgggtg ccctatagtg agtcgtattg gtaccggtag
     1141 aaaaaatgag taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
     1201 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
     1261 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatgggtaa
     1321 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagcca acacttgtca
     1381 ctactttctg ttatggtgtt caatgcttct cgagataccc agatcatatg aaacagcatg
     1441 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
     1501 atgacgggaa ctacaagaca cgtaagttta aacagttcgg tactaactaa ccatacatat
     1561 ttaaattttc aggtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
     1621 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
     1681 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttgtaa
     1741 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagaac ttcaaaatta
     1801 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
     1861 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
     1921 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
     1981 ggattacaca tggcatggac gaactataca aaagcccaca agctttggaa ttcgctagcc
     2041 ggccatacaa gtaatccgga tgatcgacgc caacgtcgtt gaattttcaa attttaaata
     2101 ctgaatattt gttttttttc ctattattta tttattctct ttgtgttttt tttcttgctt
     2161 tctaaaaaat taattcaatc caaatctaaa catttttttt tctctttccg tctcccaatt
     2221 cgtattccgc tcctctcatc tgaacacaat gtgcaagttt atttatcttc tcgctttcat
     2281 ttcattagga cgtgggggga attggtggaa gggggaaaca cacaaaagga tgatggaaat
     2341 gaaataagga cacacaatat gcaacaacat tcaattcaga aatatggagg aaggtttaaa
     2401 agaaaacata aaaatatata gaggaggaag gaaaactagt aaaaaataag caaagaaatt
     2461 aggcgaacga tgagaattgt cctcgcttgg gccctttcgt ctcgcgcgtt tcggtgatga
     2521 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     2581 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     2641 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     2701 accgcacaga tgcgtaagga gaaaataccg catcaggcgg ccttaagggc ctcgtgatac
     2761 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     2821 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2881 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     2941 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3001 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3061 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3121 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3181 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3241 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3301 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3361 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3421 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3481 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3541 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     3601 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     3661 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     3721 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     3781 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     3841 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3901 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3961 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4021 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4081 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4141 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4201 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4261 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4321 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     4381 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4441 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4501 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4561 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4621 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4681 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4741 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4801 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     4861 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     4921 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctgt
     4981 aagtttaaac atgatcttac taactaacta ttctcattta aattttcaga gcttaaaaat
     5041 ggctgaaatc actcacaacg atggatacgc taacaacttg gaaatgaaat
//
