LOCUS       sequence-315001-        1161 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for use in CID (chemically induced dimerization) or CIT (chemically 
            induced trimerization) system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Wu HD, Kikuchi M, Dagliyan O, Aragaki AK, Nakamura H, Dokholyan NV, 
            Umehara T, Inoue T
  TITLE     Rational design and implementation of a chemically inducible 
            heterotrimerization system.
  JOURNAL   Nat Methods. 2020 Sep;17(9):928-936. doi: 10.1038/s41592-020-0913-x.
            Epub 2020 Aug 3.
  PUBMED    32747768
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0913-x"; journalName: "Nat Methods"; date: 
            "2020-09"; volume: "17"; issue: "9"; pages: "928-936"
COMMENT     Sequence Label: Tom20-mCh-FKBP
FEATURES             Location/Qualifiers
     source          1..1161
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             121..828
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(266..284)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     534..553
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             841..1161
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 atggtgggtc ggaacagcgc catcgccgcg ggcgtgtgcg gtgccctctt catagggtac
       61 tgcatctact ttgaccgcaa aagacgaagt gaccccaact tcggatccgg agcaagtgga
      121 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
      181 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      241 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      301 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      361 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      421 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      481 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      541 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
      601 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
      661 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
      721 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
      781 cgcgccgagg gccgccactc caccggcggc atggacgaac tgtacaagag tgctggtggt
      841 ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag
      901 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
      961 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
     1021 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
     1081 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
     1141 gtggagcttc taaaactgga a
//
