LOCUS       sequence-315006-        1182 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for use in CIT (chemically induced trimerization) system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Wu HD, Kikuchi M, Dagliyan O, Aragaki AK, Nakamura H, Dokholyan NV, 
            Umehara T, Inoue T
  TITLE     Rational design and implementation of a chemically inducible 
            heterotrimerization system.
  JOURNAL   Nat Methods. 2020 Sep;17(9):928-936. doi: 10.1038/s41592-020-0913-x.
            Epub 2020 Aug 3.
  PUBMED    32747768
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0913-x"; journalName: "Nat Methods"; date: 
            "2020-09"; volume: "17"; issue: "9"; pages: "928-936"
COMMENT     Sequence Label: FKBP1C-CFP-Cb5
FEATURES             Location/Qualifiers
     source          1..1182
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      337..346
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(343..345,346..348,349..1059)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(388..409)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(649..668)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     996..1017
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gccaccatgg atggaaagaa atttgattcc tcccgggaca gaaacaagcc ctttaagttt
       61 atgctaggca agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg
      121 ggtcagagag ccaaactgac tatatctcca gattatgcct atggtgccac tgggcaccca
      181 ggcatcatcc caccacatgc cactctcgtc ttcgatgtgg agcttctaaa actggaacag
      241 tcgacgagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct
      301 ggtggtcggg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
      361 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      421 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      481 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      541 tggggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      601 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      661 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      721 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      781 atcagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
      841 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      901 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
      961 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1021 gccgccggga tcactctcgg catggacgag ctgtacaagt ccggactcag atctatcacc
     1081 accgtggagt ccaactcctc ctggtggacc aactgggtga tccccgccat ctccgccctg
     1141 gtggtggccc tgatgtaccg cctatacatg gccgaggact ag
//
