LOCUS       sequence-31501-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RAB14
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(604..621)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(631..648)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             667..689
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    704..728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(728..747)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(729..747)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(831..850)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa
       61 gcttcgtcat cactgaatgt tctgatagat tttcttggca gcctcaagga aggcatcttc
      121 tacattctct cccgtttttg cactcgcttc gaggaacaat aagccatttt cttcagcaaa
      181 ctgtttggct tcttcatatg taacatctct ctgtgcctcc aaatctgctt tatttcctat
      241 gagaattatt acagtatttg gattggtgag attccttgca tctgtcaacc agctgcttaa
      301 gtggttatat gtacttcttc tagtgatatc atagaccata agagctcccg cagctcctct
      361 gtagtagctc cgtgtaacag ccctaaatcg cccctgtcct gccgtatccc aaatctgcag
      421 ttttattttt tggccactaa cttcgattat tcttgtacca aattcaacac caattgtgtg
      481 aggacaatca gccataaatt ttttttctgt aaattgatga agcaagcaag attttcctac
      541 tcccatgtcc ccaataataa tatatttaaa gatgtaagag tagttgtatg gtgcagttgc
      601 catactacca cgcggaacca ggccgctgct gtgatgatga tgatgatggc tgctgcccat
      661 ggtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc
      721 acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc
      781 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
      841 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
      901 cgtg
//
