LOCUS       sequence-315014-        6222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of CD44 proteins in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Mielgo A, van Driel M, Bloem A, Landmann L, Gunthert U
  TITLE     A novel antiapoptotic mechanism based on interference of Fas 
            signaling by CD44 variant isoforms.
  JOURNAL   Cell Death Differ. 2006 Mar;13(3):465-77. doi: 
            10.1038/sj.cdd.4401763.
  PUBMED    16167069
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/sj.cdd"; 
            journalName: "Cell Death Differ"; date: "2006-03"; volume: "13"; 
            issue: "3"; pages: "465-77"
COMMENT     Sequence Label: pPGK-T7/2-CD44v2-10 (-cyt)
FEATURES             Location/Qualifiers
     source          1..6222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..500
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(36..59)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     433..452
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     intron          550..1122
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1130..1149
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1177..1196
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1177..1195
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3119..3128
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3125..3127,3128..3130,3131..3844)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3125..3127,3128..3130,3131..3841)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3431..3450)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3778..3799
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3878..3999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3915..3934)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3969..3988
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        4041..4145
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4146..4214,4215..5006)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4364..4383)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5177..5765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5666..5685
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5919..5936
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6090..6109)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
       61 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
      121 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
      181 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
      241 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
      301 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
      361 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
      421 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
      481 cctcatctcc gggcctttcg acccctaggc ttttgcaaaa agctccggat cgatcctgag
      541 aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
      601 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
      661 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
      721 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
      781 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
      841 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
      901 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
      961 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
     1021 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
     1081 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     1141 gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa
     1201 ttccgctccg gacaccatgg acaagttttg gtggcacgca gcctggggac tctgcctcgt
     1261 gccgctgagc ctggcgcaga tcgatttgaa tataacctgc cgctttgcag gtgtattcca
     1321 cgtggagaaa aatggtcgct acagcatctc tcggacggag gccgctgacc tctgcaaggc
     1381 tttcaatagc accttgccca caatggccca gatggagaaa gctctgagca tcggatttga
     1441 gacctgcagg tatgggttca tagaagggca cgtggtgatt ccccggatcc accccaactc
     1501 catctgtgca gcaaacaaca caggggtgta catcctcaca tccaacacct cccagtatga
     1561 cacatattgc ttcaatgctt cagctccacc tgaagaagat tgtacatcag tcacagacct
     1621 gcccaatgcc tttgatggac caattaccat aactattgtt aaccgtgatg gcacccgcta
     1681 tgtccagaaa ggagaataca gaacgaatcc tgaagacatc taccccagca gccctactga
     1741 tgatgacgtg agcagcggct cctccagtga aaggagcagc acttcaggag gttacatctt
     1801 ttacaccttt tctactgtac accccatccc agacgaagac agtccctgga tcaccgacag
     1861 cacagacaga atccctgcta ccagtacgtc ttcaaatacc atctcagcag gctgggagcc
     1921 aaatgaagaa aatgaagatg aaagagacag acacctcagt ttttctggat caggcattga
     1981 tgatgatgaa gattttatct ccagcaccat ttcaaccaca ccacgggctt ttgaccacac
     2041 aaaacagaac caggactgga cccagtggaa cccaagccat tcaaatccgg aagtgctact
     2101 tcagacaacc acaaggatgg ctgatgtaga cagaaatggc accactgctt atgaaggaaa
     2161 ctggaaccca gaagcacacc ctcccctcat tcaccatgag catcatgagg aagaagagac
     2221 cccacattct acaagcacaa tccaggcaac tcctagtagt acaacggaag aaacagctac
     2281 ccagaaggaa cagtggtttg gcaacagatg gcatgaggga tatcgccaaa cacccagaga
     2341 agactcccat tcgacaacag ggacagctgc agcctcagct cataccagcc atccaatgca
     2401 aggaaggaca acaccaagcc cagaggacag ttcctggact gatttcttca acccaatctc
     2461 acaccccatg ggacgaggtc atcaagcagg aagaaggatg gatatggact ccagtcatag
     2521 tacaacgctt cagcctactg caaatccaaa cacaggtttg gtggaagatt tggacaggac
     2581 aggacctctt tcaatgacaa cgcagcagag taattctcag agcttctcta catcacatga
     2641 aggcttggaa gaagataaag accatccaac aacttctact ctgacatcaa gcaataggaa
     2701 tgatgtcaca ggtggaagaa gagacccaaa tcattctgaa ggctcaacta ctttactgga
     2761 aggttatacc tctcattacc cacacacgaa ggaaagcagg accttcatcc cagtgacctc
     2821 agctaagact gggtcctttg gagttactgc agttactgtt ggagattcca actctaatgt
     2881 caatcgttcc ttatcaggag accaagacac attccacccc agtggggggt cccataccac
     2941 tcatggatct gaatcagatg gacactcaca tgggagtcaa gaaggtggag caaacacaac
     3001 ctctggtcct ataaggacac cccaaattcc agaatggctg atcatcttgg catccctctt
     3061 ggccttggct ttgattcttg cagtttgcat tgcagtcaac agagatcccc ggccggtcgc
     3121 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     3181 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     3241 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     3301 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     3361 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     3421 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     3481 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     3541 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     3601 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     3661 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3721 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3781 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3841 gtaaagcggc cgcgagatcc agatcttatt aaagcagaac ttgtttattg cagcttataa
     3901 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3961 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggt cgagcaggtg
     4021 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4081 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4141 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4201 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4261 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4321 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4381 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4441 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4501 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4561 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4621 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4681 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4741 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4801 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4861 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     4921 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4981 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5041 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5101 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5161 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5221 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5281 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5341 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5401 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5461 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5521 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5581 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5641 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5701 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5761 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5821 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     5881 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     5941 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt catttcgact
     6001 tctgaggcgg aaagaaccag gccaatatgg gatcggccat tgaacaagat ggattgcacg
     6061 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     6121 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     6181 tcaagaccga cctgtccggt gccctgaatg aacaattcta cc
//
