LOCUS       sequence-31502-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RAB14
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             651..668
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(721..739)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      735..782
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaatattttg tttactttag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggttcc gcgtggtagt atggcaactg caccatacaa ctactcttac
      121 atctttaaat atattattat tggggacatg ggagtaggaa aatcttgctt gcttcatcaa
      181 tttacagaaa aaaaatttat ggctgattgt cctcacacaa ttggtgttga atttggtaca
      241 agaataatcg aagttagtgg ccaaaaaata aaactgcaga tttgggatac ggcaggacag
      301 gggcgattta gggctgttac acggagctac tacagaggag ctgcgggagc tcttatggtc
      361 tatgatatca ctagaagaag tacatataac cacttaagca gctggttgac agatgcaagg
      421 aatctcacca atccaaatac tgtaataatt ctcataggaa ataaagcaga tttggaggca
      481 cagagagatg ttacatatga agaagccaaa cagtttgctg aagaaaatgg cttattgttc
      541 ctcgaagcga gtgcaaaaac gggagagaat gtagaagatg ccttccttga ggctgccaag
      601 aaaatctatc agaacattca gtgatgacga agcttgcggc cgcactcgag caccaccacc
      661 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      721 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      781 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
      841 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgcccctag
      901 cgcccgct
//
