LOCUS       sequence-31503-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ARF4
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..77
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             647..664
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(717..735)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ctttaagaag gagatatacc atgggcngca gccatcatca tcntcatcac agcngcggcc
       61 tggttccgcg nggtagtatg ggcctcacta tctcctccct cttctcccga ctatttggca
      121 agaagcagat gcgcattttg atggttggat tggatgctgc tggcaagaca accattctgt
      181 ataaactgaa gttaggggag atagtcacca ccattcctac cattggtttt aatgtggaaa
      241 cagtagaata taagaacatt tgtttcacag tatgggatgt tggtggtcaa gatagaatta
      301 ggcctctctg gaagcattac ttccagaata cccagggtct tatttttgtg gtagatagca
      361 acgatcgtga aagaattcag gaagtagcag atgagctgca gaaaatgctt ctggtagatg
      421 aattgagaga tgcagtgctg ctactttttg caaacaaaca ggatttgcca aatgctatgg
      481 ccatcagtga aatgacagat aaactagggc ttcagtctct tcgtaacaga acatggtatg
      541 ttcaagccac ttgtgcaaca caaggaactg gnctgtatga aggacttgac tggctgtcaa
      601 atgagctttc naaacgttga tgacgaagct tgcggccgca ctcgagcacc accaccacca
      661 ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      721 tgagcaataa ctagcat
//
