LOCUS       sequence-315033-        9527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Hara T, Takamura A, Kishi C, Iemura S, Natsume T, Guan JL, Mizushima
            N
  TITLE     FIP200, a ULK-interacting protein, is required for autophagosome 
            formation in mammalian cells.
  JOURNAL   J Cell Biol. 2008 May 5. 181(3):497-510.
  PUBMED    18443221
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2008 May 5. 181(3):497-510."
COMMENT     Sequence Label: pMXs-puro-EGFP-FAK
FEATURES             Location/Qualifiers
     source          1..9527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     347..369
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     385..401
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    427..801
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     739..755
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             join(818..820,821..823,824..1534)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(818..820,821..823,824..1534)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(863..884)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1124..1143)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1471..1492
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4763..4783)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4768..5097
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4948..5083
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5107..5706
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5107..5126)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5603..5623
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5933..6402
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6532..6549)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6703..7291)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6783..6802)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7455..8246,8247..8315))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8078..8097
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8316..8420)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             8450..9043
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    9106..9463
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
       61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
      241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc
      421 atatggccat atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct
      481 gacatgacaa gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta
      541 gtccagcacg aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg
      601 gtggtacctc acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag
      661 aacctagaac ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc
      721 aaagtagacg gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg
      781 ggtggaccat cctctagact gccggatcat cgataccatg gtgagcaagg gcgaggagct
      841 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      901 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      961 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1021 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1081 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1141 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1201 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1261 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1321 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1381 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1441 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1501 cgggatcact ctcggcatgg acgagctgta caagatcgat gaattcacta gtgttaacgg
     1561 atccatggca gctgcttatc ttgacccaaa cttgaatcac acaccaagtt cgagtactaa
     1621 gactcacctg ggtactggca tggaacggtc ccctggtgca atggaacgag tattaaaggt
     1681 ctttcatcat tttgaaagca gtagtgagcc aaccacctgg gccagtatta tcaggcatgg
     1741 agatgctact gatgtcaggg gcatcattca gaagatagtg gacagtcaca aagtaaagca
     1801 cgtggcctgc tatggatttc gcctcagtca cctgcgatcg gaggaggtgc actggctgca
     1861 cgtggacatg ggtgtctcca gtgtgaggga gaaatatgaa ctcgcccacc caccagagga
     1921 gtggaaatat gagttgagaa ttcgttactt gccaaaagga tttctaaacc agtttactga
     1981 agataagcca acattgaatt tcttctatca acaggtgaag agtgactaca tgcaagaaat
     2041 agctgatcaa gtagaccaag aaatagcttt gaagttgggt tgtttggaaa ttaggcgatc
     2101 ctattgggag atgaggggta atgccctaga gaagaagtcc aactatgaag tattagaaaa
     2161 agatgttggt ttaaagcgat tttttcctaa gagtttactg gattctgtca aggccaaaac
     2221 actaagaaag ctgatccagc aaacattcag gcagtttgcc aaccttaata gagaagaaag
     2281 catcctgaaa ttctttgaga ttctttctcc tgtgtacaga tttgacaaag agtgcttcaa
     2341 gtgtgccctt gggtcaagtt ggatcatttc tgtggaattg gcaatcggcc cagaagaagg
     2401 gatcagttac ctgacagaca aaggctgcaa tcccacacac ctagcagact ttaaccaagt
     2461 gcagaccatc cagtactcaa acagtgaaga caaagacagg aaaggaatgc tacaactcaa
     2521 aatcgctggt gctcctgagc ctctgaccgt gacggcacca tccctaacca ttgccgagaa
     2581 catggccgac ctgattgatg gatactgccg gctggtgaat ggagccacac aatctttcat
     2641 catcagaccc cagaaagaag gtgaacgggc gttgccatca ataccaaagt tggccaacag
     2701 tgaaaagcaa ggcatgcgga cacatgcagt ctctgtgtca gagacagatg actatgcaga
     2761 gatcatcgat gaggaagaca catacaccat gccctcgacc agggattatg agattcagag
     2821 agaaagaata gaacttggac gctgtattgg agaaggtcag tttggagatg tacatcaagg
     2881 cgtgtacctg agcccagaga atccagcttt ggctgttgca atcaaaacat gtaaaaactg
     2941 tacttcggac agcgtgagag agaagttcct tcaagaagcc ttaacaatgc gtcagtttga
     3001 ccatcctcac atcgtgaagc tgattggagt cattacagag aaccctgtct ggataatcat
     3061 ggagttgtgc acacttggag agctgaggtc atttttgcaa gtaagaaaat acagcttgga
     3121 cctggcatct ttgatattat atgcctatca gcttagtaca gcactcgcgt atctggagag
     3181 caaaagattt gttcacaggg acattgctgc tcggaatgtt ctggtgtctt caaatgattg
     3241 tgtaaaattg ggagactttg gattatctcg atatatggaa gacagtactt actataaagc
     3301 ttccaaagga aaattaccta ttaaatggat ggctccagag tcaatcaatt ttcgacgttt
     3361 tacctcagct agtgacgtgt ggatgtttgg tgtgtgtatg tgggagatac tgatgcatgg
     3421 cgtgaagcct tttcaaggag tgaagaacaa tgatgtgatc ggtcgaattg aaaatgggga
     3481 aagattacca atgcctccaa attgtcctcc caccctctac agccttatga cgaaatgttg
     3541 ggcctatgac cccagcaggc ggcccaggtt tactgaacta aaagctcagc tcagcacaat
     3601 cctggaggag gagaaggtgc agcaagaaga acggatgagg atggaatcca gaagacaggc
     3661 taccgtgtcc tgggactctg gagggtctga tgaagcacca cccaagccca gcagacctgg
     3721 ttatcctagc ccgagatcca gtgaaggatt ttatcccagc ccacagcaca tggtccagac
     3781 caatcactac caggtctctg gctacccggg ttcccatgga atcccagcca tggctggcag
     3841 catttaccaa ggtcaggcat ctcttctgga ccagacagaa ttgtggaatc acagacctca
     3901 ggagatgtcc atgtggcagc ccagtgtgga ggattcggca gctttggacc ttcgagggat
     3961 gggacaggtg cttccccctc acctgatgga agagcggcta atccgacagc agcaggaaat
     4021 ggaagaagac cagcgctggc tggagaagga agagcgattc ctgaaacctg atgtgaggct
     4081 ctctcgaggc agcatcgaca gggaagacgg gagttttcag ggtccgactg gaaaccaaca
     4141 catctatcag cctgtgggga agccagatcc tgcagctcca ccaaagaaac ctcctcgccc
     4201 tggagcacct ggccacctaa gcaacctgtc cagcatcagc agccctgcag acagctacaa
     4261 tgagggtgtc aagcttcagc cccaggaaat cagcccccct cccactgcca accttgaccg
     4321 gtccaatgac aaggtatatg aaaatgtgac aggcctagtg aaggctgtca tcgagatgtc
     4381 cagcaaaatc cagccagctc ctccagaaga gtacgtccct atggtgaagg aagttggcct
     4441 ggccctgcgg accttactag ccacggtgga tgagaccatt cctgctcttc cagccagcac
     4501 tcatcgagag atcgagatgg cacagaagct gctgaactcc gacttaggcg agctcatcag
     4561 caagatgaag ctggcgcagc agtacgtcat gaccagcctg cagcaggagt ataagaagca
     4621 gatgctgaca gctgctcacg ccctggctgt ggatgccaag aacctacttg atgttattga
     4681 tcaagcaaga ctgaaaatgc tagggcagac acggccacac tgaggatccc tcgagcggcc
     4741 gccagcacag tggtcgacct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     4801 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     4861 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4921 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4981 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5041 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5101 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     5161 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     5221 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     5281 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     5341 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     5401 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     5461 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     5521 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     5581 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     5641 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     5701 gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca
     5761 tcgtcgacga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
     5821 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
     5881 acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat
     5941 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     6001 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     6061 gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat
     6121 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
     6181 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
     6241 ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg
     6301 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
     6361 tctcctctga gtgattgact acccgtcagc gggggtcttt cacatgcagc atgtatcaaa
     6421 attaatttgg ttttttttct taagtattta cattaaatgg ccatagttgc attaatgaat
     6481 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6541 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6601 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6661 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6721 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6781 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6841 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6901 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6961 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7021 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7081 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7141 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7201 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7261 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7321 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7381 gatcttcacc tagatccttt tgcggccggc cgcaaatcaa tctaaagtat atatgagtaa
     7441 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7501 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7561 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7621 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7681 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7741 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7801 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7861 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7921 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7981 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8041 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8101 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8161 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8221 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8281 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8341 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8401 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgcata atgaaagacc
     8461 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata
     8521 cataactgag aatagaaaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
     8581 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     8641 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     8701 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
     8761 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     8821 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     8881 cacaacccct cactcggcgc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
     8941 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
     9001 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga
     9061 tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
     9121 ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta
     9181 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttcggaac
     9241 acccggccgc aaccctggga gacgtcccag ggacttcggg ggccgttttt gtggcccgac
     9301 ctgagtccaa aaatcccgat cgttttggac tctttggtgc acccccctta gaggagggat
     9361 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc
     9421 tttcggtttg ggaccgaagc cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt
     9481 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatat gggcccg
//
